Mostrando 741 - 760 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.26s Limitar resultados
  1. 741
    “…In contrast, highly purified MSCs, namely the rapidly expanding clones (RECs) isolated by single-cell sorting, display uniform MSCs functionality. …”
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  2. 742
  3. 743
    por Bhuiyan, Afsana, Asakawa, Shuichi
    Publicado 2023
    “…This cell-free, in vitro cloning method may be applicable for other repeat expansion diseases and be used to produce animal and cell culture models to study repeat expansion diseases in vivo and in vitro.…”
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  4. 744
    “…In this study, we applied PacBio and MinION long-read sequencing to isolates of the mentioned clone C collection. Confirmation of inversions predicted from the physical mapping data demonstrated that unbiased sequence assembly of such read datasets allows the detection of genomic inversions and the resolution of the recombination breakpoint regions. …”
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  5. 745
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  10. 750
    “…Here, we demonstrate cloning-free and precise reporter knock-in into zebrafish genes, using PCR-generated templates for homology-directed repair with short homology arms (PCR tagging). …”
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  11. 751
    “…Hybrid (regression/classification) deep learning (DL) models were developed and used to predict additional gene combinations in subsequent rounds of combinatorial cloning for increased L-threonine production based on the training data. …”
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  12. 752
  13. 753
  14. 754
    “…In the second step, we developed and validated a protocol using RGB images to classify clones, with D(2) as a dissimilarity measure. Preliminary results, with distance D, allowed to discriminate clones based on the distance of the evaluated attributes and their combinations. …”
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  15. 755
    “…Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1. MATERIALS AND METHODS: The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells. …”
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  16. 756
    “…The rapid amplification of cDNA ends (RACE) approach was used in this research to clone the CHI sequence from A. sinicus (AsiCHI). …”
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  17. 757
    “…A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an improved overlap extension cloning was developed. …”
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  18. 758
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  20. 760
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