Mostrando 841 - 860 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.31s Limitar resultados
  1. 841
    por Gilchrist, Michael J
    Publicado 2012
    “…Here I review progress in the collection of both sequence data and physical clone reagents for protein coding genes. I conclude that we have cDNA sequences for around 50% and full-length clones for about 35% of the genes in Xenopus tropicalis, and similar numbers but a smaller proportion for Xenopus laevis. …”
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  2. 842
    “…Direct cloning of PCR fragments by TA cloning or blunt end ligation are two simple methods which would greatly benefit high-throughput (HTP) cloning constructions if the efficiency can be improved. …”
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  3. 843
  4. 844
  5. 845
    “…In contrast to the classical world, an unknown quantum state cannot be cloned ideally, as stated by the no-cloning theorem. …”
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  6. 846
    por Singh, Manika Indrajit, Jain, Vikas
    Publicado 2013
    “…We report the designing of three expression vectors that can be used for rapid cloning of any blunt-end DNA segment. Only a single set of oligonucleotides are required to perform the amplification of the target DNA and its cloning in all three vectors simultaneously. …”
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  7. 847
  8. 848
    “…Without detailed genomic/gene information, in silico sequence determination is not straightforward, and full coding sequence determination typically involves time- and labor-intensive PCR-based amplification and cloning methods. RESULTS: An improved method was developed with which to determine full length gene coding sequences in silico using de novo assembly of RNA-Seq data. …”
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  9. 849
  10. 850
  11. 851
    “…We obtained 5 Coxiella burnetii isolates from samples of patients from Cayenne and found an epidemic clone circulating in Cayenne. This clone has caused pneumonia and endocarditis and seems to be more virulent than previously described strains.…”
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  12. 852
    “…The knowledge of evolution of chy genes in photosynthetic eukaryotes provided information of gene cloning and functional investigation of chy genes in algae in the future.…”
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  13. 853
    “…In the present study, we were able to solve those problems utilizing our modified real competitive PCR method with cloned competitors (mrcPCR). First, the mrcPCR for ERBB2 copy number was established, and the results were comparable to current standard methods but with a shorter assay time and a lower cost. …”
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  14. 854
    por Matroudi, S., Zamani, M.R., Motallebi, M.
    Publicado 2008
    “…From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. …”
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  15. 855
    “…Here, we present an efficient protocol that facilitates the generation of these complex constructs. The golden GATEway cloning approach presented here combines two established cloning methods, namely golden Gate cloning and Multisite Gateway(TM) cloning. …”
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  16. 856
    “…The specific RAPD bands of D. longan cultivars from Guangxi, with size ranging from 500 bp to 900 bp were gel-purified, cloned and sequenced. Four clones named LY2-1, LY4-7, LY4-8 and LY5-2 were identified. …”
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  17. 857
  18. 858
    “…This work focuses on MCAP cloning and optimization of heterologous expression in Pichia pastoris, and characterization of the enzyme. …”
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  19. 859
  20. 860
    “…BACKGROUND: DNA fragments carrying internal recognition sites for the restriction endonucleases intended for cloning into a target plasmid pose a challenge for conventional cloning. …”
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