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Mostrando 861 - 880 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.25s Limitar resultados
  1. 861
    “…Especially in case of long inserts and applications requiring high-throughput, this approach is limited by the number of available unique restriction sites and the need for designing individual cloning strategies for each project. Several alternative cloning systems have been developed in recent years to overcome these issues, including the type IIS enzyme based Golden Gate technique. …”
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    GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis
  2. 862
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    Molecular Cloning and Expression Analysis of Triticale Phytocystatins During Development and Germination of Seeds
  3. 863
    “…We show here, by whole-genome sequencing, that most strains collected from February 2010 through March 2013, and associated with 2 outbreaks of lymph node tuberculosis in children, belong to a unique epidemic clone within M. canettii. Evolution of this clone, which has been recovered regularly since 1983, may mimic the birth of M. tuberculosis. …”
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    Progenitor “Mycobacterium canettii” Clone Responsible for Lymph Node Tuberculosis Epidemic, Djibouti
  4. 864
    “…METHODOLOGY/PRINCIPAL FINDINGS: A CsGrb2 cDNA clone retrieved from the C. sinensis transcriptome encoded a polypeptide with a SH3-SH2-SH3 structure. …”
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    Molecular Cloning and Characterization of Growth Factor Receptor Bound-Protein in Clonorchis sinensis
  5. 865
    “…Individuals with the same genotypes formed distinct clonal lineages: one major clone was represented by 72 individuals, an intermediate clone was represented by 21 individuals, and nine other clones were rare and represented by one or several individuals. …”
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    Clonal Diversity and Clone Formation in the Parthenogenetic Caucasian Rock Lizard Darevskia dahli
  6. 866
    “…One cloned pup (Clone 1) was healthy, but the other (Clone 2) had a birth weight of only 320 g and cleft palate, failure of preputial closure at the ventral distal part, and persistent penile frenulum. …”
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    Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog
  7. 867
    “…MATERIALS AND METHODS: L-asparaginase from E. carotovora NCYC 1526 (ErA) was cloned and expressed in Escherichia coli strain BL21 (DE3). …”
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    Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli
  8. 868
    “…METHODS: The protein was cloned as His-tagged fusion protein and purified. …”
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    Cloning, Expression and Purification of L. Donovani Specific Antigen for Serodiagnosis of Visceral Leishmaniasis
  9. 869
    “…Cloned animals usually exhibited many defects in physical characteristics or aberrant epigenetic reprogramming, especially in some important organ development. …”
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    Osteoponin Promoter Controlled by DNA Methylation: Aberrant Methylation in Cloned Porcine Genome
  10. 870
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    Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system
  11. 871
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    Cloning, expression and purification of functionally active human angiopoietin-like protein 2
  12. 872
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    Molecular Cloning and Characterization of Novel Phytocystatin Gene from Turmeric, Curcuma longa
  13. 873
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    Cloning and characterization of newly isolated lipase from Enterobacter sp. Bn12
  14. 874
    “…BACKGROUND: Epigenetic modifications (especially altered DNA methylation) resulting in altered gene expression may be one reason for development failure or abnormalities in cloned animals, but the underlying mechanism of the abnormal phenotype in cloned piglets remains unknown. …”
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    Dysregulation of genome-wide gene expression and DNA methylation in abnormal cloned piglets
  15. 875
    “…The product was then cloned into pTZ57R plasmid. The cloned cDNA was digested out and ligated into pGEX-5x-1 expression vector. …”
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    Molecular cloning of Reteplase and its expression in E. coli using tac promoter
  16. 876
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    Cloning and expression of the NS1 protein of dengue virus in a prokaryotic system
  17. 877
    “…The ribT gene nucleotide sequence was amplified and cloned into the standard high-copy expression vector pET15b and then expressed after induction with IPTG in E. coli BL21 (DE3) strain cells containing the inducible phage T7 RNA polymerase gene. …”
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    Possible Function of the ribT Gene of Bacillus subtilis: Theoretical Prediction, Cloning, and Expression
  18. 878
    “…While cloned T cells are valuable tools for the exploration of immune responses against viruses and tumours, current cloning methods do not allow inferences to be made about the function and phenotype of a clone's in vivo precursor, nor can precise cloning efficiencies be calculated. …”
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    High Efficiency Ex Vivo Cloning of Antigen-Specific Human Effector T Cells
  19. 879
    “…Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2’-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. …”
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    Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos
  20. 880
    por Roshan, A., Jones, P. H., Greenman, C. D.
    Publicado 2014
    “…Biological tools such as genetic lineage tracing, three-dimensional confocal microscopy and next-generation DNA sequencing are providing new ways to quantify the distribution of clones of normal and mutated cells. Understanding population-wide clone size distributions in vivo is complicated by multiple cell types within observed tissues, and overlapping birth and death processes. …”
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    Exact, time-independent estimation of clone size distributions in normal and mutated cells
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