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Mostrando 881 - 900 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.23s Limitar resultados
  1. 881
    “…METHODS: This study examined synergy in neutralization by six monoclonal antibodies targeting different domains in gp120 and gp41 and assayed in pairwise combination against 11 HIV-1 clade B strains, either Env pseudoviruses (PV, n = 5) or transmitted/founder infectious molecular clones (T/F IMCs, n = 6). Three of the early-infection env tested as PV were juxtaposed with T/F viruses derived from the same three patients, respectively. …”
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    Synergy in monoclonal antibody neutralization of HIV-1 pseudoviruses and infectious molecular clones
  2. 882
    por Takatsu, Kiyoshi
    Publicado 2014
    “…This is a perspective based on the paper “Cloning of complementary DNA encoding T cell-replacing factor and identity with B cell growth factor II,” by Kinashi et al. (1). …”
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    Revisiting the Identification and cDNA Cloning of T Cell-Replacing Factor/Interleukin-5
  3. 883
    “…The vectors combine the advantage of efficient uracil excision reaction-based cloning and Cre-LoxP-mediated marker recycling system. …”
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    EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae
  4. 884
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    Cloning and heterologous expression of cellulose free thermostable xylanase from Bacillus brevis
  5. 885
    “…Third, compared with the WTSIMGP data set, the mERG‐Nie clone is likely to represent the wild‐type mouse sequence and physiology. …”
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    Mouse ERG K(+) Channel Clones Reveal Differences in Protein Trafficking and Function
  6. 886
    “…Finally, confirmation of successful cloning is explained using a fluorescent gene of interest and murine target cells. …”
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    Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering
  7. 887
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    Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria
  8. 888
    “…RESULTS: A total of 61 SmWRKYs were cloned from S. miltiorrhiza. Multiple sequence alignment showed that SmWRKYs could be classified into 3 groups and 8 subgroups. …”
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    Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza
  9. 889
    “…The aim of this study was to clone, express and purify recombinant ESAT-6 andCFP-10 proteins of M. tuberculosis in soluble form. …”
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    Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens
  10. 890
    “…The bacterial flora was analyzed according to the clone library method using amplified fragments of the 16S ribosomal RNA gene with universal primers. …”
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    Bacteriological Assessment of Healthcare-Associated Pneumonia Using a Clone Library Analysis
  11. 891
    “…We describe solid-phase cloning (SPC) for high-throughput assembly of expression plasmids. …”
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    Solid-phase cloning for high-throughput assembly of single and multiple DNA parts
  12. 892
    “…Our cloning-free CRISPR/Cas system facilitates rapid one-step generation of cassette knock-in mice, accelerating functional genomic research by providing various in vivo genetic tools. …”
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    Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice
  13. 893
    “…RESULTS: Assays for SOD activity demonstrated that the cloned C. burnetii insert codes for a SOD that was active over a wide range of pH and inhibitable with 5 mM H(2)O(2) and 1 mM sodium diethyldithiocarbamate, a characteristic of Cu/ZnSODs that distinguishes them from Fe or Mn SODs. …”
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    Cloning, expression, and characterization of a Coxiella burnetii Cu/Zn Superoxide dismutase
  14. 894
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    Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120
  15. 895
    “…Replication dynamics in vitro of these optimized clones were not statistically different from the parental clones. …”
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    Generation and characterization of a SIVmac239 clone corrected at four suboptimal nucleotides
  16. 896
    “…Therefore, the aim of this study was to develop a self-cloning-based positive selection system for the breeding of P. ellipsoidea. …”
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    Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea
  17. 897
    por Kilaru, S., Steinberg, G.
    Publicado 2015
    “…Generation of these tools requires efficient cloning methods, as vector construction is often a rate-limiting step. …”
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    Yeast recombination-based cloning as an efficient way of constructing vectors for Zymoseptoria tritici
  18. 898
    “…As a way to circumvent the quantum no-cloning theorem, approximate quantum cloning protocols have received wide attention with remarkable applications. …”
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    Memory-built-in quantum cloning in a hybrid solid-state spin register
  19. 899
    “…We have cloned a novel human mixed-lineage kinase gene, MLK4. …”
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    Cloning and Initial Functional Characterization of Mlk4α and Mlk4β
  20. 900
    “…BirA amplicon harboring a GS-linker at its C-terminal was cloned into NdeI-XhoI sites of pET24a(+) vector under control of T7 promoter and upstream of the vector-derived 6xHis-tag. pET24-BirA transformed BL21-cells were induced for protein expression by IPTG and analyzed by SDS-PAGE and Western blotting. …”
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    Isolation, cloning, and expression of E. coli BirA gene for biotinylation applications
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