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  1. 981
    “…Interleukin-1 receptor activated kinases (IRAKs) play crucial roles in the Toll-like receptor (TLR) mediated signal transduction pathways that control host innate immune responses. Here we report the cloning of an IRAK1 cDNA (named ScIRAK1) from the mandarin fish. …”
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    Cloning of IRAK1 and its upregulation in symptomatic mandarin fish infected with ISKNV
  2. 982
    por Brooks, Colin G.
    Publicado 1983
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    Reversible induction of natural killer cell activity in cloned murine cytotoxic T lymphocytes
  3. 983
    “…IL-18 binding protein (IL-18BP) is an intrinsic inhibitor of IL-18 that possesses higher affinity to IL-18. In this study, we cloned and characterized IL-18BP in giant panda (AmIL-18BP) from the spleen. …”
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    Cloning and characterization of giant panda (Ailuropoda melanoleuca) IL-18 binding protein
  4. 984
    por Esposito, Alessandro
    Publicado 2021
    “…Growing experimental evidence also suggests that cooperation between cancer clones is important as well for the maintenance of tumour heterogeneity and tumour progression. …”
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    Cooperation of partially transformed clones: an invisible force behind the early stages of carcinogenesis
  5. 985
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    Efficiency of various cloned DNA probes for detection of bovine viral diarrhea viruses()
  6. 986
    “…We report the cloning, expression and characterization of biologically active feline tumour necrosis factor-α (fTNF-α). …”
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    Cloning, expression and characterization of biologically active feline tumour necrosis factor-α
  7. 987
    “…This problem is frequently observed during assembly of infectious full-length virus clones. If the clone is constructed for transcription in vivo, interrupting the virus sequence with an intron can solve the toxicity problem. …”
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    Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones
  8. 988
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    Toward the Development of an Infectious cDNA Clone of a Human Enteric Coronavirus
  9. 989
    por Paul, Martin, Weller, Michael G.
    Publicado 2020
    “…The latter can also be used to select clones of desired cross-reactivity properties. In this paper, a model system with two excellent clones against carbamazepine, two weak clones, and blank supernatant containing fetal bovine serum was designed to examine the effectiveness of the new system. …”
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    Antibody Screening by Microarray Technology—Direct Identification of Selective High-Affinity Clones
  10. 990
    “…In this study, we designed different constructs of the Leucine zipper domain to find the over –expressed construct for further studies. We successfully cloned the C-terminal TRAIP containing the leucine zipper domain. …”
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    Molecular cloning, expression, overproduction and characterization of human TRAIP Leucine zipper protein
  11. 991
    “…To further evaluate the pathogenicity of the obtained PCV3 infectious clones, this study established an animal model of Kunming mice infected with PCV3. …”
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    A Novel Technique for Constructing Infectious Cloning of Type 3 Porcine Circovirus
  12. 992
    “…Single-cell cloning (SCC) is a critical step in generating monoclonal cell lines, which are widely used as in vitro models and for producing proteins with high reproducibility for research and the production of therapeutic drugs. …”
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    A Microfluidic Single-Cell Cloning (SCC) Device for the Generation of Monoclonal Cells
  13. 993
    “…Stable, functional mobility of the vaccine-TLR protein binding interface was confirmed by the Normal Mode Analysis. The in-silico cloning model demonstrated the efficacy of the construct vaccine along with the identified epitopes against SARS-CoV-2. …”
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    A SARS-CoV-2 vaccine candidate: In-silico cloning and validation
  14. 994
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    Chaetocin Improves Pig Cloning Efficiency by Enhancing Epigenetic Reprogramming and Autophagic Activity
  15. 995
    por Horns, Felix, Quake, Stephen R.
    Publicado 2020
    “…Antibodies function by binding to antigens. Antibodies must be cloned and expressed to determine their binding characteristics, but current methods for high-throughput antibody sequencing yield antibody DNA pooled from many cells and do not readily permit cloning of antibodies from single B cells. …”
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    Cloning antibodies from single cells in pooled sequence libraries by selective PCR
  16. 996
    “…In this paper, we present transcriptomic data and analysis of three H. brasiliensis clones using tissue from latex and bark tissues collected from 10-year-old plant. …”
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    Transcriptome dataset from bark and latex tissues of three Hevea brasiliensis clones
  17. 997
    “…We sequenced the western gorilla (Gorilla gorilla) HoxA cluster region using seven fosmid clones, and found that the total tiling path sequence was 214,185 bp from the 5' non-genic region of HoxA1 to the 3' non-genic region of Evx1. …”
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    Nucleotide sequencing of the HoxA gene cluster using Gorilla fosmid clones
  18. 998
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    Molecular Cloning and Functional Analysis of the NPR1 Homolog in Kiwifruit (Actinidia eriantha)
  19. 999
    por Hunker, Avery C., Zweifel, Larry S.
    Publicado 2020
    “…This protocol optimizes a workflow for the design, cloning, and validation of sgRNAs delivered by AAVs in vivo that can be applied to any target gene in the CNS of rat or mouse model systems and can be adapted to Cre or Flp driver lines using AAV-FLEX-SaCas9-sgRNA or AAV-FLEXfrt-SaCas9-sgRNA, respectively. …”
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    Protocol to Design, Clone, and Validate sgRNAs for In Vivo Reverse Genetic Studies
  20. 1000
    “…To understand the cultivation characteristics of a CAM plant pitaya, it is necessary to clarify the characteristics of PEPC in this species. Here, we cloned three PEPC cDNAs in pitaya, HuPPC1, HuPPC2, and HuPPC3, which encode 942, 934, and 966 amino acid residues, respectively. …”
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    Molecular Cloning of Novel-Type Phosphoenolpyruvate Carboxylase Isoforms in Pitaya (Hylocereus undatus)
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