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  1. 1081
    “…Multiple transcript variants of PIWIL1 were cloned from feline testes cDNA. One isoform shows high homology to PIWIL1 from other mammals, however, the other has characteristics of a “slicer null” isoform, lacking the domain required for endonuclease activity. …”
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    cDNA Cloning of Feline PIWIL1 and Evaluation of Expression in the Testis of the Domestic Cat
  2. 1082
    “…Pseudomonas aeruginosa ST308 clone has been reported to carry carbapenemase genes such as bla(IMP) and bla(VIM) but has been rarely associated with bla(NDM-1). …”
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    Dissemination of Pseudomonas aeruginosa bla(NDM-1)-Positive ST308 Clone in Singapore
  3. 1083
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    Editorial: 30th anniversary of the molecular cloning and identification of the Na-Cl cotransporter, NCC
  4. 1084
    “…This system utilizes induced sensitivity to the toxic amino acid analog p-chlorophenylalanine (4-CP) as a negative selection mechanism and was developed for counterselection-based cloning-independent markerless mutagenesis (CIMM). …”
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    Adaptation of Prokaryotic Toxins for Negative Selection and Cloning-Independent Markerless Mutagenesis in Streptococcus Species
  5. 1085
    “…In this review, some breakthroughs in rice yield traits and a series of achievements in molecular design breeding in China in recent years are presented; the identification and cloning of functional genes related to yield traits and the development of molecular markers of rice functional genes are summarized, with the intention of playing a reference role in the following molecular design breeding work and how to further improve rice yield.…”
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    Advances in cloning functional genes for rice yield traits and molecular design breeding in China
  6. 1086
    “…Here, we report the cloning of the Pst resistance gene YrNAM in this region using the method of sequencing trait-associated mutations (STAM). …”
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    Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM
  7. 1087
    “…We then detail clone isolation and GOI knockout validation. For complete details on the use and execution of this protocol, please refer to Wu et al.(1)…”
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    Generation of gene-of-interest double allele knockout clones in primary human T cells by CRISPR
  8. 1088
    “…In this study, we report the cloning of DNMT1 (4833 bp in length) from porcine alveolar macrophages (PAMs). …”
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    A Porcine DNMT1 Variant: Molecular Cloning and Generation of Specific Polyclonal Antibody
  9. 1089
    “…Remarkably, GDR clones were more sensitive than GDS clones to the mitochondrial respiratory chain complex I inhibitor metformin, thus suggesting a potential therapeutic strategy to target the OXPHOS-metabolic dependency of this subpopulation. …”
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    Anti-VEGF therapy selects for clones resistant to glucose starvation in ovarian cancer xenografts
  10. 1090
    “…This national survey investigated the current practice in Switzerland by collecting participants' opinions on paroxysmal nocturnal hemoglobinuria (PNH) clone assessment and clinical practice. AIM: This study aimed to investigate clinical indications prompting PNH clones' assessment and physician's accessibility of a flow cytometry facility, and also to understand clinical attitudes on the follow-up (FU) of patients with PNH clones. …”
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    Swiss Survey on current practices and opinions on clinical constellations triggering the search for PNH clones
  11. 1091
    “…Here, we show that copy number boundaries identify treatment-selected clones with putatively distinct lethal trajectories.…”
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    Copy number architectures define treatment-mediated selection of lethal prostate cancer clones
  12. 1092
    “…We have developed a screening platform capable of providing genotyped functional data from a total of 9216 soluble, individual antigen binding fragment (Fab) clones by employing next-generation sequencing (NGS) with hierarchical indexing. …”
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    Genotyped functional screening of soluble Fab clones enables in-depth analysis of mutation effects
  13. 1093
    por Lan, Tian, Guillot, Adrien, Tacke, Frank
    Publicado 2023
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    Somatic mutant clone screening: scan for novel NASH target genes in mouse liver
  14. 1094
    “…Thus, our study shows that the H3K9me3-repressed genes contain developmentally required genes, and the incomplete activation of such genes results in preimplantation arrest of cloned embryos.…”
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    Incomplete activation of Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryos
  15. 1095
    “…Molecular cloning and controlled expression remain challenging when the target gene encodes a protein that is toxic to the host. …”
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    A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli
  16. 1096
    por Sorida, Masato, Bonasio, Roberto
    Publicado 2023
    “…Golden Gate Assembly is an efficient and rapid cloning method but requires dedicated vectors. Here, we modified Golden Gate to expand its compatibility to a broader range of destination vectors while maintaining its strengths. …”
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    An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly
  17. 1097
    “…Source code: https://github.com/bdj34/cloneRate/.…”
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    cloneRate: fast estimation of single-cell clonal dynamics using coalescent theory
  18. 1098
    “…These selected excellent clones can serve as candidate materials for the improvement and transformation of Alnus cremastogyne seed orchards. …”
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    Phenotypic Variation Analysis and Excellent Clone Selection of Alnus cremastogyne from Different Provenances
  19. 1099
    “…Moreover, the expression pattern analysis of NHX, BZIP, ST, and KTrans transcript revealed their upregulation in the Al-Qassim clone under salt-stress conditions, suggesting that these genes play a role in this clone’s tolerance to salt-induced stress. …”
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    Differential Morpho-Physiological and Biochemical Responses of Duckweed Clones from Saudi Arabia to Salinity
  20. 1100
    “…CONCLUSION: These findings revealed the occurrence of high-risk ST1193 clone among ESBL-EC isolates from pregnant women and newborn colonization in China. …”
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    Emergence of ST1193 Clone in Maternal and Neonatal ESBL-Producing E. coli Isolates
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