Mostrando 1,101 - 1,120 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.24s Limitar resultados
  1. 1101
    “…In this laboratory study, we raised two groups of first instar nymphs belonging to two clones of the pea aphid Acyrthosiphon pisum, on fava beans Vicia faba. …”
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  2. 1102
    “…Here, we sequence, assemble, and annotate clones held at three laboratories providing reference-level assemblies. …”
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  3. 1103
    por Zhang, Pan, Zhang, Bo, Fu, Yu, Li, Pan, Zhang, Hao
    Publicado 2023
    “…Therefore, with the aim of further characterizing this gene and its protein, we cloned the entire 3286-bp mRNA sequence of the porcine AACS, and the encoded AACS protein is a hydrophilic protein without a signal peptide or transmembrane sequence. …”
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  4. 1104
    “…First, the GmNHL1 gene was successfully cloned, and bioinformatic analysis revealed multiple cis-acting elements which are related to adversity stress and involved in the oxidative response in the promoter region. …”
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  5. 1105
  6. 1106
  7. 1107
    “…Here we describe the basis for homologous recombination cloning in E. coli, the available tools and resources, together with a protocol for long range cloning and manipulation of an Arabidopsis thaliana gene locus, to create constructs co-ordinately driven by locus-specific regulatory elements.…”
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  8. 1108
    “…It is well suited to generate polymorphic markers for selected chromosomal regions, contigs of overlapping clones and yields sufficient high quality sequence data to develop amplicons greater than 250 bases.…”
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  9. 1109
    por Yamada, Hiroshi Y., Gorbsky, Gary J.
    Publicado 2006
    “…We developed a mammalian cell-based cDNA cloning method to isolate proteins and protein fragments whose expression inhibits colony formation in the presence of microtubule inhibitors. …”
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  10. 1110
    por Sadowski, Paul D
    Publicado 2003
    “…BACKGROUND: While conventional cloning methods using restriction enzymes and polynucleotide ligase are adequate for most DNAs, fragments made by the polymerase chain reaction are difficult to clone because the amplifying DNA polymerase tends to add untemplated nucleotides to the 3'-termini of the amplified strands. …”
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  11. 1111
    “…BACKGROUND: Whereas the molecular assembly of protein expression clones is readily automated and routinely accomplished in high throughput, sequence verification of these clones is still largely performed manually, an arduous and time consuming process. …”
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  12. 1112
    “…The TATA of two clones from the same murine methylcholanthrene-induced fibrosarcoma have been investigated by immunizing syngeneic mice with irradiated cells of one or both clones and challenging them 14 days later with viable cells. …”
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  13. 1113
    “…A cDNA library constructed from mRNA from a human breast carcinoma metastasis was screened with a polyclonal antibody to deglycosylated human milk fat globule membrane, resulting in the isolation of eight clones from a total of 10(5) plaques. One of these (J16) was identified as lactoferrin. …”
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  14. 1114
  15. 1115
    por Suzuki, N.
    Publicado 1983
    “…In contrast, tumour-cell release efficiency (number of blood-borne clonogenic tumour-cells) correlates well with the difference in spontaneous lung metastasis efficiencies of the cell clones FSA1231 and FSA1233. Also, the different tumour-cell release efficiencies could explain the discrepancy between artificial and spontaneous metastasis of these cell clones. …”
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  16. 1116
    “…In order to understand its gene expression response to environmental contaminants, we cloned 4032 cDNAs or expressed sequence tags (ESTs) from two E. fetida libraries enriched with genes responsive to ten ordnance related compounds using suppressive subtractive hybridization-PCR. …”
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  17. 1117
    Publicado 1982
    “…Clones of nontransformed hormone-responsive bone cells have been isolated in vitro from mixed cell populations of fetal rat calvaria. …”
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  18. 1118
  19. 1119
    Publicado 1990
    “…Integrin heterodimers mediate a variety of adhesive interactions, including neuronal attachment to and process outgrowth on laminin. We report here the cloning and primary sequence of an M-200 kD integrin alpha subunit that associates with the integrin beta 1 subunit to form a receptor for both laminin and collagen. …”
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  20. 1120
    Publicado 1991
    “…In contrast to gamma/delta + clones (but in line with established data), none of five CD4+ or CD8+ TCR-alpha/beta + clones were activated by any of the tested individual anti-CD2 mAbs. …”
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