Mostrando 1,141 - 1,160 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.33s Limitar resultados
  1. 1141
  2. 1142
    “…The cloned NS cell lines expressed a surface phenotype (2, 6) similar to that reported previously for cloned NK cells (Thy-1(+), asialo-GM1(+), Ig(-), Lyt-1(-), Lyt-2(-), Ia(-), MAC-1(-)) (7-9). …”
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  3. 1143
    Publicado 1988
    “…The purpose of this study was to examine the effect of lymphokines produced by clones of Th1 cells (IL-2 and IFN-gamma), Th2 cells (IL-4), and APC (IL-1) on the proliferative response of Th1 and Th2 cells after antigenic stimulation. …”
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  4. 1144
    Publicado 1989
    “…These mutant lymphocytes were selectively cloned, taking advantage of their resistance to a purine analogue, 6- thioguanine. …”
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  5. 1145
    Publicado 1989
    “…The partial amino acid sequences of both subunits were used to construct oligonucleotides for the screening of several cDNA libraries, including one derived from human-induced umbilical cord mononuclear cells. A cDNA clone was isolated corresponding to EPO. The nucleotide sequence revealed an open reading frame of 2,106 bp, corresponding to a prosequence, L chain, and H chain, in this order. …”
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  6. 1146
    Publicado 1989
    “…We have isolated a 725-bp full-length cDNA clone for the human eosinophil cationic protein (ECP). …”
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  7. 1147
    Publicado 1995
    “…The antigen gene was isolated from the E. coli clone that, when ingested by the macrophages, allowed generation of the appropriate peptide/MHC class II complex and T cell activation. …”
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  8. 1148
    “…We have cloned the squid neuronal Na(+)–Ca(2+) exchanger, NCX-SQ1, expressed it in Xenopus oocytes, and characterized its regulatory and ion transport properties in giant excised membrane patches. …”
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  9. 1149
  10. 1150
    “…We have developed a comprehensive resource devoted to biologists wanting to optimize the use of gene trap clones in their experiments. We have processed 300 602 such clones from both public and private projects to generate 28 199 ‘UniTraps’, i.e. distinct collections of unambiguous insertions at the same subgenic region of annotated genes. …”
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  11. 1151
  12. 1152
    “…Somatic nuclear transfer is a cloning technique that shows great promise in the application to regenerative medicine. …”
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  13. 1153
    Publicado 1992
    “…A cDNA clone coding for a membrane proteoglycan core protein was isolated from a neonatal rat Schwann cell cDNA library by screening with an oligonucleotide based on a conserved sequence in cDNAs coding for previously described proteoglycan core proteins. …”
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  14. 1154
  15. 1155
    “…BACKGROUND: The mitochondrial DNA (mtDNA) of the cloned sheep "Dolly" and nine other ovine clones produced by somatic cell nuclear transfer (SCNT) was reported to consist only of recipient oocyte mtDNA without any detectable mtDNA contribution from the nucleus donor cell. …”
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  16. 1156
    “…Indeed many standard molecular strategies, including molecular cloning, studies of DNA-protein interactions, and analysis of nucleic acid structures, would be virtually impossible without these versatile enzymes. …”
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  17. 1157
    por Mead, Edward Andrew, Tu, Zhijian
    Publicado 2008
    “…RESULTS: We report the cloning and characterization of 27 distinct miRNAs from 17-day old An. stephensi female mosquitoes. …”
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  18. 1158
  19. 1159
    “…RESULTS: Here, we present a USER Friendly cloning based technique that allows single step cloning of the two required homologous recombination sequences into different sites of a recipient vector. …”
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  20. 1160
    “…Based on this property, a cloning strategy called ‘Golden Gate’ cloning was devised that allows to obtain in one tube and one step close to one hundred percent correct recombinant plasmids after just a 5 minute restriction-ligation. …”
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