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  1. 1181
    “…The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. …”
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    Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
  2. 1182
    “…Main clinical manifestations were pharyngitis and scarlet fever, but this clone also caused invasive disease: two cases of streptococcal toxic shock syndrome, one arthritis, and one celullitis with a fatal outcome. …”
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    Spread of a highly mucoid Streptococcus pyogenes emm3/ST15 clone
  3. 1183
    “…A homology search of each ORF predicted that orf3 encoded l-phenylserine dehydrogenase. Hence, orf3 was cloned and overexpressed in Escherichia coli cells and recombinant ORF3 was purified to homogeneity and characterized. …”
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    Identification, Cloning, and Characterization of l-Phenylserine Dehydrogenase from Pseudomonas syringae NK-15
  4. 1184
    “…Amino acid sequence information obtained by Edman sequencing and mass spectrometry analysis was used to clone the gene encoding BspRI. The bspRIR gene is located adjacently to the gene of the cognate modification methyltransferase and encodes a 304 aa protein. …”
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    BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism
  5. 1185
    “…A full-length cDNA fragment coding for UGDH was cloned from the cambial region of 6-month-old E. grandis saplings by RT-PCR. …”
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    Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA
  6. 1186
    “…Furthermore, full-length cDNAs encoding type 1 potassium channel toxins from three species of Stichodactylidae and three species of Thalassianthidae were cloned by a combination of RT-PCR, 3′RACE and 5′RACE. …”
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    Screening and cDNA Cloning of Kv1 Potassium Channel Toxins in Sea Anemones
  7. 1187
    “…The abundance of established HTLV-1 clones is determined by genomic features of the host DNA flanking the provirus. …”
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    The host genomic environment of the provirus determines the abundance of HTLV-1–infected T-cell clones
  8. 1188
    “…The emergence and spread of virulent clones expressing Panton-Valentine leukocidin (PVL) is an additional cause for concern. …”
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    A Field Guide to Pandemic, Epidemic and Sporadic Clones of Methicillin-Resistant Staphylococcus aureus
  9. 1189
    “…BACKGROUND: Obtaining single parasite clones is required for many techniques in malaria research. …”
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    An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites
  10. 1190
    “…The TCR usages were Vα12D-3-01+Jα11-01 and Vβ12-1-01+Dβ1-01+J2-7-01 for clone #14, Vα13D-1-02+Jα34-02 and Vβ13-1-02+Dβ2-01+Jβ2-7-01 for clone #15, and Vα16D+Jα45-01 and Vβ12-1-01+Dβ1-01+Jβ2-5-01 for clone #23. …”
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    Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen
  11. 1191
    “…The expression of this protein was low when cloned under the T7 promoter without any fusion tags. …”
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    Cloning, Soluble Expression and Purification of High Yield Recombinant hGMCSF in Escherichia coli
  12. 1192
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    Susceptibility of HBZ and Tax expressing primary HTLV-1+ T cell clones to CTL lysis
  13. 1193
    “…Here we report the purification, characterization, identification, and cloning of lysoplasmalogenase. Rat liver microsomal lysoplasmalogenase was solubilized with octyl glucoside and purified 500-fold to near homogeneity using four chromatography steps. …”
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    Purification, Identification, and Cloning of Lysoplasmalogenase, the Enzyme That Catalyzes Hydrolysis of the Vinyl Ether Bond of Lysoplasmalogen
  14. 1194
    “…There was a twofold increase in the biosurfactant and esterase activities after the successful cloning of the biosurfactant genes from Bacillus subtilis SK320 into E.coli. …”
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    Enhanced biosurfactant production through cloning of three genes and role of esterase in biosurfactant release
  15. 1195
    “…Amylase activity showed 3 clones with higher enzymatic activity compared with the wild type. …”
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    Cloning and Expression of Randomly Mutated Bacillus subtilis α-Amylase Genes in HB101
  16. 1196
    “…METHODS: Full-length duck VLDLR cDNA was obtained by comparative cloning and rapid amplification of cDNA ends (RACE). …”
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    Cloning and expression profiling of the VLDLR gene associated with egg performance in duck (Anas platyrhynchos)
  17. 1197
    “…To investigate PERV integration sites, 45 PERV-positive BAC clones, including 12 PERV-A, 16 PERV-B, and 17 PERV-C clones, were identified from the NIH miniature pig BAC library. …”
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    Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
  18. 1198
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    Cloning and Characterization of the ζ-Carotene Desaturase Gene from Chlorella protothecoides CS-41
  19. 1199
    “…Nevertheless, a delay of 4–5 days is observed in comparison with normal testis differentiation in XY males. By positional cloning, we demonstrate that the PIS mutation is an 11.7-kb regulatory-deletion affecting the expression of two genes, PISRT1 and FOXL2 which could act synergistically to promote ovarian differentiation. …”
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    Positional cloning of the PIS mutation in goats and its impact on understanding mammalian sex-differentiation
  20. 1200
    por Ghodsi, S, Gharavi, S, Ghadam, P
    Publicado 2010
    “…To confirm the expression of the cloned gene, SDS-PAGE was carried out and production of an approximately 11 KD recombinant tagged protein was confirmed for the cloned hbs gene. …”
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    Cloning the hbs gene from Bacillus subtilis and expression of the HBsu protein in Escherichia coli
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