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  1. 1301
    “…The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. …”
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    Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
  2. 1302
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    Cloning and Characterization of a Human Genomic Sequence that Alleviates Repeat-Induced Gene Silencing
  3. 1303
    “…The aims of present research were cloning, expression, purification of fHbp and confirmation of the interaction between serum factor H (fH) and produced factor H binding protein. …”
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    Cloning, expression and purification of the factor H binding protein and its interaction with factor H
  4. 1304
    “…Insufficient attention has been given to molecular and sequence-based methods necessary for identifying and tracking resistant clones in Africa and more research is needed in this area.…”
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    Fluoroquinolone-Resistant Enteric Bacteria in Sub-Saharan Africa: Clones, Implications and Research Needs
  5. 1305
    “…However, the N-glycan profile of another MSC clone (Y202), which cannot differentiate into osteoblasts, was not significantly different from that of the clone that can. …”
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    Glycan Profiling Shows Unvaried N-Glycomes in MSC Clones with Distinct Differentiation Potentials
  6. 1306
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    Presence of a widely disseminated Listeria monocytogenes serotype 4b clone in India
  7. 1307
    “…We identified a zoonotic S. suis clone that diverged from a non-zoonotic clone by means of gene loss, a capsule switch, and acquisition of a two-component signalling system in the late 19th century, when foreign pig breeds were introduced. …”
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    An emerging zoonotic clone in the Netherlands provides clues to virulence and zoonotic potential of Streptococcus suis
  8. 1308
    “…MATERIALS AND METHODS: In the current experimental study, the r-B-flagellin gene was isolated from the P. aeruginosa PAO1 strain by PCR. It was cloned into the pET-28a vector and then transformed into the E. coli BL21 strain. …”
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    Cloning, Expression, and Purification of Pseudomonas aeruginosa Flagellin, and Characterization of the Elicited Anti-Flagellin Antibody
  9. 1309
    “…B. cereus ATCC 14579 ColA was cloned to express recombinant wildtype ColA (ColA(wt)) and mutated to a proteolytically inactive (ColA(E501A)) version. …”
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    Cloning, Purification and Characterization of the Collagenase ColA Expressed by Bacillus cereus ATCC 14579
  10. 1310
    “…CONCLUSIONS: Epidemic KMRSA clones were genetically similar to major clones identified from the USA, with the exception of KMRSA-2, which had the SCCmec III type. …”
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    Prevalence of Major Methicillin-Resistant Staphylococcus aureus Clones in Korea Between 2001 and 2008
  11. 1311
    “…Here, we worked out a simple cloning strategy to develop multisite small interfering RNA (siRNA) cassette from different genes by two cloning steps. …”
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    Design, simplified cloning, and in-silico analysis of multisite small interfering RNA-targeting cassettes
  12. 1312
    “…In 2011, we crossed a cloned male and normal female by natural breeding and produced ten offspring. …”
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    Reproductive ability of a cloned male detector dog and behavioral traits of its offspring
  13. 1313
    “…These five STs have been recombining recently, leading to a shared pool of allelic variants potentially contributing to their increased disease propensity. The oldest clone, ST1, has spread globally; between 1940 and 2000, four new clones have emerged in Europe, which show long-distance, rapid dispersal. …”
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    Multiple major disease-associated clones of Legionella pneumophila have emerged recently and independently
  14. 1314
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    Cloning and expression of Aca f 1: a new allergen of Acacia farnesiana pollen
  15. 1315
    “…Unattended sensor nodes can be comprised and cloned to destroy the network topology. This paper proposes a novel distributed protocol and management technique for the detection of mobile replicas to tolerate node failures. …”
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    An Improved Mobility-Based Control Protocol for Tolerating Clone Failures in Wireless Sensor Networks
  16. 1316
    “…On the universal host V. faba all aphid clones triggered only low SA levels initially, but induced clone-specific patterns of SA and JA-Ile later on. …”
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    Modulation of Legume Defense Signaling Pathways by Native and Non-native Pea Aphid Clones
  17. 1317
    “…This siRNA-mediated inhibition of Xist significantly increased the total cell number per cloned blastocyst and significantly improved the birth rate of cloned healthy piglets. …”
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    Effects of RNAi-mediated knockdown of Xist on the developmental efficiency of cloned male porcine embryos
  18. 1318
    “…Here we describe the cloning and characterization of Pax6 gene from the blind hydrothermal vent tubeworm Ridgeia piscesae (RpPax6). …”
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    Cloning and Functional Analysis of Pax6 from the Hydrothermal Vent Tubeworm Ridgeia piscesae
  19. 1319
    “…PO is typically synthesized as a zymogen called prophenoloxidase (PPO) and requires the proteolytic activation to function. We here cloned full-length cDNA for 3 previously unidentified PPOs, which we named OfPPO1a, OfPPO1b, and OfPPO3, from Asian corn borer, Ostrinia furnacalis (Gunée), in addition to the previously known OfPPO2. …”
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    Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée)
  20. 1320
    “…A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. …”
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    A catalog of human cDNA expression clones and its application to structural genomics
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