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  1. 1321
    “…The set of five Ti-plasmids are easily and efficiently used for Gateway cloning and transient transformation of N. benthamiana leaves.…”
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    A Split-GFP Gateway Cloning System for Topology Analyses of Membrane Proteins in Plants
  2. 1322
    “…Here we describe the cloning of the cDNAs encoding these subunits from adult rhesus monkey testis RNA. …”
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    Cloning of a novel inhibin alpha cDNA from rhesus monkey testis
  3. 1323
    “…This work is the first documentation of molecular cloning and characterization of luxI homolog from C. neteri.…”
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    Cloning and Characterization of the Autoinducer Synthase Gene from Lipid-Degrading Bacterium Cedecea neteri
  4. 1324
    “…METHODS: The 1170 bp coding sequence of the CSUI_005805 gene was PCR amplified and cloned into the bacterial expression vector pQE-31. …”
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    Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein
  5. 1325
    “…A single extra-intestinal pathogenic Escherichia coli (ExPEC) clone, named sequence type (ST) 131, is responsible for millions of global antimicrobial-resistant (AMR) infections annually. …”
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    Escherichia coli ST131: a multidrug-resistant clone primed for global domination
  6. 1326
    “…Molecular cloning methods based on primer and overlap-extension PCR are widely used due to their simplicity, reliability, low cost and high efficiency. …”
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    Mega primer-mediated molecular cloning strategy for chimaeragenesis and long DNA fragment insertion
  7. 1327
    “…Our study confirms that horses carry an equine-specific clone of MRSA CC398 that can be transmitted to veterinary personnel, and reveals that these animals are exposed to MRSA and MSSA clones that are likely to originate from livestock and humans, respectively.…”
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    Horses in Denmark Are a Reservoir of Diverse Clones of Methicillin-Resistant and -Susceptible Staphylococcus aureus
  8. 1328
    “…A multidrug-resistant Staphylococcus capitis clone, NRCS-A, has been isolated from neonatal intensive care units in 17 countries throughout the world. …”
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    Worldwide Endemicity of a Multidrug-Resistant Staphylococcus capitis Clone Involved in Neonatal Sepsis
  9. 1329
    “…The sequence of a full-length cDNA clone contained an open reading frame encoding a polypeptide of 198 amino acid residues, with a calculated molecular weight of 22,150 Da. …”
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    Cloning, expression and antioxidant activity of a thioredoxin peroxidase from Branchiostoma belcheri tsingtaunese
  10. 1330
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    Molecular cloning and functional expression of geranylgeranyl pyrophosphate synthase from Coleus forskohlii Briq
  11. 1331
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    Recurrent detection of VIM-1-producing Escherichia coli clone in German pig production
  12. 1332
    “…Cloning of multiple genes in a single vector has greatly facilitated both basic and translational studies that require co-expression of multiple factors or multi-units of complex protein. …”
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    Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector
  13. 1333
    por Harayama, Takeshi, Riezman, Howard
    Publicado 2017
    “…Therefore, a method to discriminate accurately mutated clones easily and quickly is crucial to accelerate the research using CRISPR/Cas9. …”
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    Detection of genome-edited mutant clones by a simple competition-based PCR method
  14. 1334
    por Udo, Edet E., Al-Sweih, Noura
    Publicado 2017
    “…Other clones were isolated sporadically. The number of MRSA isolates increased from two in 2006 to 22 in 2011 with a peak of 43 in 2008. …”
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    Dominance of community-associated methicillin-resistant Staphylococcus aureus clones in a maternity hospital
  15. 1335
    “…An unexpected functional and phenotypic plasticity of pancreatic TICs in vivo underlies the recruitment of inactive TIC clones in serial xenografts. The observed clonal succession of TIC activity in serial xenotransplantation is in stark contrast to the continuous activity of limited numbers of self‐renewing TICs within a fixed cellular hierarchy observed in other epithelial cancers and emphasizes the need to target TIC activation, rather than a fixed TIC population, in PDAC.…”
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    Succession of transiently active tumor‐initiating cell clones in human pancreatic cancer xenografts
  16. 1336
    por Gupta, Sanjeev K., Shukla, Pratyoosh
    Publicado 2017
    “…Currently, there are few innovative avenues are studies for cloning, upstream, and purification through efficient bioprocess development. …”
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    Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Review
  17. 1337
    “…Compared with the ST258 clone, capsulated ST307 isolates showed higher resistance to complement-mediated killing. …”
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    Diversity, virulence, and antimicrobial resistance of the KPC-producing Klebsiella pneumoniae ST307 clone
  18. 1338
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    Frutapin, a lectin from Artocarpus incisa (breadfruit): cloning, expression and molecular insights
  19. 1339
    “…We developed a pipeline by combining multivariate analysis of clone shape with tissue mechanical simulations. We examined clones with four different genotypes within Drosophila wing imaginal discs: wild-type, tartan (trn) overexpression, hibris (hbs) overexpression, and Eph RNAi. …”
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    Inference of Cell Mechanics in Heterogeneous Epithelial Tissue Based on Multivariate Clone Shape Quantification
  20. 1340
    “…In the present study, we further developed this model by deriving a molecular clone SIVsm804E-CL757 (CL757). This clone induced neurological disorders in high frequencies but without rapid disease progression and thus is more reflective of the tempo of neuroAIDS in HIV-infection. …”
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    A SIV molecular clone that targets the CNS and induces neuroAIDS in rhesus macaques
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