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  1. 1381
    “…Here, we have established an antigen-specific single B cell sorting and monoclonal antibody (mAb) cloning platform for analyzing immunization- or viral infection-elicited antibody response at the clonal level in guinea pigs. …”
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    Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs
  2. 1382
    “…In addition, we closely analyzed the improperly expressed histone lysine methyltransferases and histone lysine demethylases during cell reprogramming in cloned embryos. In summary, we identified altered gene expression profiles in porcine cloned pre-implantation embryos in comparison to normal in vivo embryos. …”
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    Characterization and comparative analyses of transcriptomes of cloned and in vivo fertilized porcine pre-implantation embryos
  3. 1383
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    Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.
  4. 1384
    “…Here we show that hypervirulent Lm clones, particularly CC1, are strongly associated with dairy products, whereas hypovirulent clones, CC9 and CC121, are associated with meat products. …”
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    Hypervirulent Listeria monocytogenes clones’ adaption to mammalian gut accounts for their association with dairy products
  5. 1385
    “…Given how rapidly such pstS-null strains have expanded in Australia, the emergence of this clone in Scotland raises concerns for a potential outbreak.…”
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    Emergence of an Australian-like pstS-null vancomycin resistant Enterococcus faecium clone in Scotland
  6. 1386
    “…Therefore, in the present study, we cloned a novel carrier cell with the aim of improving the antitumor effects. …”
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    Cloning of carrier cells infected with oncolytic adenovirus driven by midkine promoter and biosafety studies
  7. 1387
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    Unusual, stable replicating viruses generated from mumps virus cDNA clones
  8. 1388
    “…Infectious clone vectors used widely in genetic research. While constructing soybean mosaic virus (SMV) clone vectors, we found that transformed Agrobacterium grew significantly different depending on the viral strains used. …”
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    Discovery of the Agrobacterium growth inhibition sequence in virus and its application to recombinant clone screening
  9. 1389
    “…In this study, we combined transcriptome analysis, rapid amplification of cDNA ends-cloning (RACE-cloning), expression profiling, and heterologous functional assay to identify SWEET genes that may have potential roles during flower opening and sexual reproduction in Jasminum sambac . …”
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    Cloning and Functional Assessments of Floral-Expressed SWEET Transporter Genes from Jasminum sambac
  10. 1390
    “…QuickStep is a cloning method that allows seamless point integration of a DNA sequence at any position within a target plasmid using only Q5 High-Fidelity DNA Polymerase and DpnI endonuclease. …”
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    PTO-QuickStep: A Fast and Efficient Method for Cloning Random Mutagenesis Libraries
  11. 1391
    “…However, the successful derivation of knockout clones can be technically challenging and may be complicated by multiple factors, including incomplete target ablation and interclonal heterogeneity. …”
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    Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
  12. 1392
    “…METHODS: MuV-S79 cDNA clone was assembled into a full-length plasmid by means of the GeneArt™ High-Order Genetic Assembly System, and was rescued via reverse genetic technology. …”
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    Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA
  13. 1393
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    The genome of Populus alba x Populus tremula var. glandulosa clone 84K
  14. 1394
    “…Rhesus macaques (Macaca mulatta) are used as a human-relevant animal species for the evaluation of vaccines and as a source for cloning monoclonal antibodies (mAbs) that are highly similar to human-derived antibodies. …”
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    Phenotypic Characterization of Chinese Rhesus Macaque Plasmablasts for Cloning Antigen-Specific Monoclonal Antibodies
  15. 1395
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    Invasions of gladiolus rust in North America are caused by a widely-distributed clone of Uromycestransversalis
  16. 1396
    “…Here, we report a simplified method to clone and express amiRNAs in Arabidopsis and rice based on the engineered Arabidopsis miR319a or rice miR528 precursor, which harbor restriction sites to facilitate one-step cloning of a single PCR product. …”
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    Engineered Artificial MicroRNA Precursors Facilitate Cloning and Gene Silencing in Arabidopsis and Rice
  17. 1397
    “…There is no requirement for cloning in generation of the integration donor, instead the desired integration donor is produced as a polymerase chain reaction (PCR) product, flanked by the Cas9 recognition sequences of the target locus. …”
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    Fast and cloning‐free CRISPR/Cas9‐mediated genomic editing in mammalian cells
  18. 1398
    “…Particularly, the molecular processes underlying the interplay between clones of the same cultivar and environment were poorly investigated. …”
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    Distinct Metabolic Signals Underlie Clone by Environment Interplay in “Nebbiolo” Grapes Over Ripening
  19. 1399
    “…The 7-h cloning process only needs ∼1 h of labor. Moreover, this method can also clone mRNA, simplifying the need to prepare two cloning systems for small RNA and mRNA; the barcoded PCR primers are also compatible with non-cDNA cloning applications, including the preparation of genomic libraries. …”
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    A convenient strategy to clone small RNA and mRNA for high-throughput sequencing
  20. 1400
    “…Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization — the model for acute infection in humans — showing their large diversity. …”
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    Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
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