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  1. 1421
    “…RESULTS: The exochitinase CHI from P. chitinolyticus strain UMBR 0002 was successfully cloned and heterologously expressed in E. coli Rosetta-gami B (DE3). …”
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    Cloning, expression and characterization of a chitinase from Paenibacillus chitinolyticus strain UMBR 0002
  2. 1422
    “…Taken together, the results indicated that the cloned MdNAC genes were expressed constitutively in all examined tissues. …”
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    Cloning, sequencing, and expression analysis of 32 NAC transcription factors (MdNAC) in apple
  3. 1423
    “…Phylogenetic reconstruction identified ancestral clones, with attendant driver mutations in RCC tumor suppressors (including VHL, SETD2, PBRM1, MTOR) and independent subclonal populations in the metastases of all 3 patients. …”
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    OTHR-17. PHYLOGENETIC RESOLUTION OF TISSUE-SPECIFIC METASTOGENIC CLONES IN RENAL CELL CARCINOMA
  4. 1424
    “…A fluoroquinolone-resistant triple-mutant clone of S. Typhi 4.3.1.2 previously associated with gatifloxacin treatment failure in Nepal was implicated in 18% of enteric fever cases, indicating ongoing inter-regional circulation. …”
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    Persistent circulation of a fluoroquinolone-resistant Salmonella enterica Typhi clone in the Indian subcontinent
  5. 1425
    “…Power calculations based on the measured variance attributable to major sources of experimental error indicated that typical eQTL explaining 10% of the variation in expression of a gene would usually require at least eight biological replicates of each clone. Scanning across credible intervals with this approach is not recommended.…”
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    Pitfalls in Single Clone CRISPR-Cas9 Mutagenesis to Fine-Map Regulatory Intervals
  6. 1426
    “…The reservoir has an estimated half-life of 44 mo and is largely composed of clones of infected CD4(+) T cells. The long half-life appears to result in part from expansion and contraction of infected CD4(+) T cell clones. …”
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    Antigen-responsive CD4(+) T cell clones contribute to the HIV-1 latent reservoir
  7. 1427
    por ÖZAVCI, Hamza, KAPLAN, Mustafa
    Publicado 2019
    “…MATERIALS AND METHODS: LtTSA was amplified by PCR using the specific primers of TSA gene and cloned into the pcDNA3.1 vector. The cloning was confirmed by PCR screening, restriction enzyme reactions, and DNA sequence analysis. …”
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    Cloning and molecular characterization of thiol-specific antioxidant gene of Leishmania tropica Turkey isolate
  8. 1428
    “…Current consensus for gene cloning in wheat is to manually perform many steps in a long bioinformatics pipeline. …”
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    AutoCloner: automatic homologue-specific primer design for full-gene cloning in polyploids
  9. 1429
    “…The developmental dynamics of cloned Mexican bighorn sheep (Ovis canadensis mexicana) embryos were evaluated based on morphological quality standards. …”
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    Developmental dynamics of cloned Mexican bighorn sheep embryos using morphological quality standards
  10. 1430
    “…We further expressed most of the cloned SmPDEs in two pde1Δ/pde2Δ strains of Saccharomyces cerevisiae and some also in a specialised strain of Schizosacharomyces pombe. …”
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    Cloning and functional complementation of ten Schistosoma mansoni phosphodiesterases expressed in the mammalian host stages
  11. 1431
    por Campbell, A Malcolm, Eckdahl, Todd T
    Publicado 2018
    “…rClone Red is a low-cost and student-friendly research tool that has been used successfully in undergraduate teaching laboratories. …”
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    rClone Red facilitates bacterial gene expression research by undergraduates in the teaching laboratory
  12. 1432
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    Single-Round Infectious Particle Production by DNA-Launched Infectious Clones of Bungowannah Pestivirus
  13. 1433
    “…We characterize the behavior of FastClone in identifying subclones using stage III colon cancer primary tumor samples as well as simulated data. …”
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    FastClone is a probabilistic tool for deconvoluting tumor heterogeneity in bulk-sequencing samples
  14. 1434
    “…For characterizing the genome of semi-cloned embryos further, we establish ESC lines from semi-cloned blastocysts. …”
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    Polyploidy of semi-cloned embryos generated from parthenogenetic haploid embryonic stem cells
  15. 1435
    “…We first defined the cloning conditions regarding homology arm-length and input adenoviral genome amounts. …”
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    High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75
  16. 1436
    “…RCK’s reconstructions of clone- and haplotype-specific karyotypes will aid further studies of the role of intra-tumor heterogeneity in cancer development and response to treatment. …”
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    Reconstruction of clone- and haplotype-specific cancer genome karyotypes from bulk tumor samples
  17. 1437
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    Cloning and Functional Characterization of Dihydroflavonol 4-Reductase Gene Involved in Anthocyanin Biosynthesis of Chrysanthemum
  18. 1438
    “…We report the generation of a full-length infectious cDNA clone for porcine deltacoronavirus strain USA/IL/2014/026. …”
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    Development and utilization of an infectious clone for porcine deltacoronavirus strain USA/IL/2014/026
  19. 1439
    “…Here, the production of cellulose nanofibers from the resistant Ulmus minor clone Ademuz was evaluated for the first time. Both mechanical (PFI refining) and chemical (TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-mediated oxidation) pretreatments were assessed prior to microfluidization, observing not only easier fibrillation but also better optical and barrier properties for elm nanopapers compared to eucalyptus ones (used as reference). …”
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    Cellulose Nanofibers from a Dutch Elm Disease-Resistant Ulmus minor Clone
  20. 1440
    “…Here we present data on the ability of bNAbs to inhibit capture (1 h) or replication (48 h) of purified primary acute or chronic HIV or infectious molecular clones (IMCs) in human peripheral blood mononuclear cells (PBMCs) as quantified by qRT-PCR. …”
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    Primary HIV-1 and Infectious Molecular Clones Are Differentially Susceptible to Broadly Neutralizing Antibodies
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