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  1. 1581
    “…TRAIL-R3, a new member of the TRAIL receptor family, has been cloned and characterized. TRAIL-R3 encodes a 299 amino acid protein with 58 and 54% overall identity to TRAIL-R1 and -R2, respectively. …”
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    Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family
  2. 1582
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    Cloning, purification and preliminary crystallographic analysis of the Bacillus subtilis GTPase YphC–GDP complex
  3. 1583
    “…ESTs from full-length cDNA clones are especially powerful for accurate molecular annotation. …”
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    Functional annotation of 19,841 Populus nigra full-length enriched cDNA clones
  4. 1584
    “…B. subtilis thiD has been cloned and the protein has been overexpressed in Escherichia coli, purified and subsequently crystallized in a binary complex with ADP and Mg(2+). …”
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    Cloning, purification and preliminary crystallographic analysis of a putative pyridoxal kinase from Bacillus subtilis
  5. 1585
    Publicado 1995
    “…The voltage-, time-, and K(+)-dependent properties of a G protein- activated inwardly rectifying K+ channel (GIRK1/KGA/Kir3.1) cloned from rat atrium were studied in Xenopus oocytes under two-electrode voltage clamp. …”
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    Intrinsic gating properties of a cloned G protein-activated inward rectifier K+ channel
  6. 1586
    Publicado 1996
    “…The effects of strontium (Sr2+; 7-50 mM) on five different cloned rat K channels (Kv1.1, Kv1.5, Kv1.6, Kv2.1, and Kv3.4), expressed in oocytes of Xenopus laevis, were investigated with a two-electrode voltage clamp technique. …”
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    Surface Charges of K channels. Effects of strontium on five cloned channels expressed in Xenopus oocytes
  7. 1587
    por Xu, Ruqiang, Li, Qingshun Quinn
    Publicado 2008
    “…BACKGROUND: In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. …”
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    Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway(® )TOPO vector system
  8. 1588
    “…METHODS: We utilized cultures of the human colon carcinoma cell clones Caco-2/AQ, COGA-1A and COGA-13, all of which expressed differentiation and proliferation markers typical of distinct stages of tumour progression. …”
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    Clone-specific expression, transcriptional regulation, and action of interleukin-6 in human colon carcinoma cells
  9. 1589
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    Cloning and Sequencing of Protein Kinase cDNA from Harbor Seal (Phoca vitulina) Lymphocytes
  10. 1590
    “…We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic mouse. …”
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    Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone
  11. 1591
    “…RESULTS: A novel gene, K31, was identified as an up-regulated gene in fish cross-subfamily cloned embryos using SSH approach and RACE method. …”
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    Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos
  12. 1592
    Publicado 1991
    “…cDNA clones encoding the alpha chain of the murine lymphocyte-Peyer's patch adhesion molecule (LPAM), which is associated with lymphocyte homing, have been isolated by screening with the human VLA-4 (alpha 4h) probe. …”
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    Cloning and expression of cDNAs for the alpha subunit of the murine lymphocyte-Peyer's patch adhesion molecule
  13. 1593
    “…A total of 86 BAC clones were anchored on the linkage map with at least one BAC clone on each linkage group. …”
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    Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, Lates calcarifer
  14. 1594
    “…RESULTS: Here we describe cloning of the human granulocyte colony-stimulating factor coding DNA sequence, protein expression in E. coli BL21(DE3) host cells in the absence of isopropyl-β-D-thiogalactopyranoside (IPTG) induction, efficient isolation and solubilization of inclusion bodies by a multi-step washing procedure, and a purification protocol using a single cationic exchange column. …”
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    Human granulocyte colony stimulating factor (hG-CSF): cloning, overexpression, purification and characterization
  15. 1595
    “…Here, we describe a method allowing direct cloning of a transgene in the E3 region of the Ad5 genome already containing the immediate early CMV promoter upstream of three unique restriction sites. …”
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    A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter
  16. 1596
    “…The accumulation of STAT1 mRNA in different developmental stages, as determined by real time RT-PCR, increased with development. CONCLUSION: Recent cloning of various cytokine genes and the STAT1 gene of olive flounder here suggest that fish also use the highly specialized JAK-STAT pathway for cytokine signaling. …”
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    Molecular cloning and expression analysis of the STAT1 gene from olive flounder, Paralichthys olivaceus
  17. 1597
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    Positional Cloning of “Lisch-like”, a Candidate Modifier of Susceptibility to Type 2 Diabetes in Mice
  18. 1598
    “…The problem lies in the reduced availability of suitable RE recognition sites with an increasing number of cloning events and or vector size. This report details a technically simple, directional cloning solution using REs with compatible cohesive ends that are repeatedly destroyed and simultaneously re-introduced with each round of cloning. …”
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    An Infinitely Expandable Cloning Strategy plus Repeat-Proof PCR for Working with Multiple shRNA
  19. 1599
    “…They are rescued from transfected plasmids encoding the eight influenza virus gene segments, which have been cloned using restriction endonucleases and DNA ligation. …”
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    Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification
  20. 1600
    “…BACKGROUND: Cloning of parasites by limiting dilution is an essential and rate-limiting step in many aspects of malaria research including genomic and genetic manipulation studies. …”
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    A highly sensitive, PCR-based method for the detection of Plasmodium falciparum clones in microtiter plates
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