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  1. 1621
    por Lomholt, Hans B., Kilian, Mogens
    Publicado 2010
    “…An unexpected geographically and temporal widespread dissemination of some clones was demonstrated. The population comprised three major divisions, one of which, including an epidemic clone, was strongly associated with moderate to severe acne while others were associated with health and opportunistic infections. …”
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    Population Genetic Analysis of Propionibacterium acnes Identifies a Subpopulation and Epidemic Clones Associated with Acne
  2. 1622
    “…FINDINGS: Here, we report the cloning and characterization by experimental and computational studies of a novel alternative transcript of the human CHD7 (named CHD7 CRA_e), which lacks most of its coding exons. …”
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    Cloning and characterization of a novel alternatively spliced transcript of the human CHD7 putative helicase
  3. 1623
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    Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms
  4. 1624
    “…The nitric oxide synthase-like protein from Bacillus cereus (bcNOS) has been cloned, expressed, and characterized. This small hemeprotein (356 amino acids in length) has a mass of 43 kDa and forms a dimer. …”
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    Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus
  5. 1625
    “…The amplified chains were cloned into pGEM-T Easy vector. Specific primers were designed and used in an amplification and chain linkage strategy, obtaining the scFv, which in turn was cloned into pAE vector. …”
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    Cloning approach and functional analysis of anti-intimin single-chain variable fragment (scFv)
  6. 1626
    “…A superoxide dismutase gene from thermotolerant Bacillus sp. MHS47 (MnSOD47) was cloned, sequenced, and expressed. The gene has an open reading frame of 612 bp, corresponding to 203 deduced amino acids, with high homology to the amino acid sequences of B. thuringiensis (accession no. …”
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    Cloning, Expression, and Characterization of Thermotolerant Manganese Superoxide Dismutase from Bacillus sp. MHS47
  7. 1627
    “…The gustavus cDNA sequence was firstly cloned from the oriental river prawn and the pattern of gene expression was described during oocyte maturation, embryonic development, and in other tissues. …”
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    cDNA Cloning and Expression Analysis of Gustavus Gene in the Oriental River Prawn Macrobrachium nipponense
  8. 1628
    “…Clones were screened by PCR, as well as Southern blotting, which revealed clones missed by PCR due to sequence mismatches with the primers. …”
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    Cloning of the Repertoire of Individual Plasmodium falciparum var Genes Using Transformation Associated Recombination (TAR)
  9. 1629
    Publicado 1999
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    Incident of "human cloning" from Kyunghee University Medical Center. The Korean Academy of Medical Sciences.
  10. 1630
    “…Recently, polyploid gibel carp has been revealed to possess coexisting dual modes of unisexual gynogenesis and sexual reproduction and to have numerous various clones. Using sexual reproduction mating between clone D female and clone A male and subsequent 7 generation multiplying of unisexual gynogenesis, we have created a novel clone strain with more than several hundred millions of individuals. …”
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    A novel nucleo-cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp
  11. 1631
    “…Prevalence and identity of bacteria was analyzed using a 16S rRNA gene clone library technique. RESULTS: A total of 112 different species were identified from 335 clones sequenced. …”
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    Analysis of microbiota associated with peri-implantitis using 16S rRNA gene clone library
  12. 1632
    “…In conclusion, it is shown that this random PCR cloning approach can be used for the characterization of new PRRSV strains of unknown genetic background.…”
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    Characterization of a circulating PRRSV strain by means of random PCR cloning and full genome sequencing
  13. 1633
    “…RESULTS: In this study, recA genes from Deinococcus geothermalis and Deinococcus murrayi, bacteria that are slightly thermophilic and extremely γ-radiation resistant, were isolated, cloned and expressed in E. coli. After production and purification, the biochemical properties of DgeRecA and DmuRecA proteins were determined. …”
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    RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation
  14. 1634
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    Online Artículo Texto
    Cloned HTLV-1+CD4+, but not CD8+, T-cells display an oncogenic miRNome
  15. 1635
    “…A 5706-bp DNA fragment was detected by Southern blotting, selected from a genomic DNA library, and cloned to the vector, pMS2KI. Two adjacent complete open reading frames within pMS2KI were sequenced, characterized, and identified as caroS2K and caroS2I, which respectively encode the killing protein and immunity protein. …”
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    Cloning, purification, and functional characterization of Carocin S2, a ribonuclease bacteriocin produced by Pectobacterium carotovorum
  16. 1636
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    The complexity of Rhipicephalus (Boophilus) microplus genome characterised through detailed analysis of two BAC clones
  17. 1637
    “…In the present study, we cloned the porcine CatSper 1-4 genes, analysed their spatial expression in various organs and temporal expression in the testes from birth until sexual maturity in Meishan boars. …”
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    Molecular cloning, spatial and temporal expression analysis of CatSper genes in the Chinese Meishan pigs
  18. 1638
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    Use of SSR-Tools for clone certification in Uruguayan Eucalyptus grandis and Eucalyptus dunnii breeding programs
  19. 1639
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    Online Artículo Texto
    Local and systemic defense response in Aspen clones: contrasting defense response to biotrophic and necrotrophic pathogens
  20. 1640
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    Molecular Cloning and Characterization of Two Genes Encoding Dihydroflavonol-4-Reductase from Populus trichocarpa
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