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  1. 1641
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    Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine)
  2. 1642
    “…The present study aimed at developing and validating the in vitro and vivo models of TYST and evaluating the sensitivity of TYST to treatments, by cloning human TYST cells and investigating the histology, ultra-structure, growth kinetics and expression of specific proteins of cloned cells. …”
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    Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor
  3. 1643
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    Primary Transgenic Bovine Cells and Their Rejuvenated Cloned Equivalents Show Transgene-Specific Epigenetic Differences
  4. 1644
    “…WA gene successfully cloned into E. coli BL21 (DE3) showed high asparaginase activity with a specific activity of 17.3 IU/mg protein.…”
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    Cloning, expression and characterization of l-asparaginase from Withania somnifera L. for large scale production
  5. 1645
    “…However, it is currently unknown how BAG-1 expression is regulated at the molecular level, especially in cancer cells. Here we reported to clone a novel down-regulated BAG-1 expression gene named FLJ20420 using hBAG-1 promoter as a probe to screen Human Hela 5′ cDNA library by Southernwestern blot. …”
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    Cloning and Functional Analysis of FLJ20420: A Novel Transcription Factor for the BAG-1 Promoter
  6. 1646
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    Cloning and characterization of miRNAs from maize seedling roots under low phosphorus stress
  7. 1647
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    Expanding the set of rhodococcal Baeyer–Villiger monooxygenases by high-throughput cloning, expression and substrate screening
  8. 1648
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    Phage Display-based Strategies for Cloning and Optimization of Monoclonal Antibodies Directed against Human Pathogens
  9. 1649
    “…The second model, accounting for different clones, suggested an enhanced transmissibility of clone 3 (transmission rate 0.047/day [0.018–0.091], with a single-admission reproduction number of 0.81 [0.30–1.56]). …”
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    Identifying More Epidemic Clones during a Hospital Outbreak of Multidrug-Resistant Acinetobacter baumannii
  10. 1650
    “…In an effort to isolate novel meiotic mutants that are severely defective in chromosome segregation and/or exchange, we employed a germline clone screen of the X chromosome of Drosophila melanogaster. …”
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    A Germline Clone Screen on the X Chromosome Reveals Novel Meiotic Mutants in Drosophila melanogaster
  11. 1651
    “…The preparation of cDNA expression clones, transfection and image acquisition are described in detail below. …”
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    A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
  12. 1652
    “…Second, the suitable genes for trastuzumab heavy and light chains were designed, synthesized, and cloned in the prokaryotic vector. These fragments individually were PCR amplified and cloned into pcDNA™ 3.3-TOPO(®) and pOptiVEC™ TOPO(®) shuttle vectors, using standard methods. …”
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    In silico design, construction and cloning of Trastuzumab humanized monoclonal antibody: A possible biosimilar for Herceptin
  13. 1653
    “…RESULTS: In this study, complete cDNA encoding laccase (Lac) from white rot fungus Cyathus bulleri was amplified by RACE-PCR, cloned and expressed in the culture supernatant of Pichia pastoris under the control of the alcohol oxidase (AOX)1 promoter. …”
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    Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase
  14. 1654
    por Treangen, Todd J, Phillippy, Adam M
    Publicado 2012
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    Irreconcilable differences: divorcing geographic mutation and recombination rates within a global MRSA clone
  15. 1655
    “…The glk A gene was cloned in the Sma I site of pQE 30, sequenced (Accession number: JN645812) and expressed in E. coli DH5α. …”
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    Cloning, expression and characterization of glucokinase gene involved in the glucose-6- phosphate formation in Staphylococcus aureus
  16. 1656
    “…BACKGROUND: The cloning of gene sequences forms the basis for many molecular biological studies. …”
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    Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption
  17. 1657
    “…Somatic cell nuclear transfer, a technique used to generate clone embryos by transferring the nucleus of a somatic cell into an enucleated oocyte, is an excellent approach to study the reprogramming of the nuclei of differentiated cells. …”
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    The transcriptomic architecture of mouse Sertoli cell clone embryos reveals temporal–spatial-specific reprogramming
  18. 1658
    “…The β-glucosidase gene bgl3a from Myceliophthora thermophila, member of the fungal glycosyl hydrolase (GH) family 3, was cloned and expressed in Pichia pastoris. The mature β-glucosidase gene, which results after the excision of one intron and the secreting signal peptide, was placed under the control of the strong alcohol oxidase promoter (AOX1) in the plasmid pPICZαC. …”
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    Cloning, expression and characterization of an ethanol tolerant GH3 β-glucosidase from Myceliophthora thermophila
  19. 1659
    “…METHODS: The isolation and cloning of gene PF3D7_18400 was done by amplification from cDNA from mixed stage parasites of P. falciparum. …”
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    Cloning and characterization of bifunctional enzyme farnesyl diphosphate/geranylgeranyl diphosphate synthase from Plasmodium falciparum
  20. 1660
    por Kim, Seong-Bin, Timmusk, Salme
    Publicado 2013
    “…To mediate homologous recombination and replacement of the targeted genes with the antibiotic resistance genes nucleotide sequences of the α-and β-amylase genes were cloned into the plasmid flanking the antibiotic resistance genes. …”
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    A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa
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