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  1. 1721
    “…The group A Streptococcus (GAS) M1T1 clone emerged in the 1980s as a leading cause of epidemic invasive infections worldwide, including necrotizing fasciitis and toxic shock syndrome123. …”
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    Transfer of scarlet fever-associated elements into the group A Streptococcus M1T1 clone
  2. 1722
    “…Using functional expression cloning and T-cell receptor (TCR) transgenic mice, we have identified cyclooxygenase 2/prostaglandin-endoperoxide synthase 2 (COX-2) as resistance factor against the cytotoxicity induced by activated, antigen-specific T cells. …”
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    Functional expression cloning identifies COX-2 as a suppressor of antigen-specific cancer immunity
  3. 1723
    “…Escherichia coli sequence type 131 (ST131) is a clone of uropathogenic E. coli that has emerged rapidly and disseminated globally in both clinical and community settings. …”
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    Lineage-Specific Methyltransferases Define the Methylome of the Globally Disseminated Escherichia coli ST131 Clone
  4. 1724
    “…Traditional cut-paste DNA cloning is often limited by the availability of restriction enzyme sites. …”
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    Complementary annealing mediated by exonuclease: a method for seamless cloning and conditioning site-directed mutagenesis
  5. 1725
    por Aw, Rochelle, Polizzi, Karen M.
    Publicado 2016
    “…BACKGROUND: Multiple cognate gene copy clones have often been used in order to increase the yield of recombinant protein expression in the yeast Pichia pastoris. …”
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    Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris
  6. 1726
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    Cloning and characterization of filamentous temperature-sensitive protein Z from Xanthomonas oryzae pv. Oryzae
  7. 1727
    “…However, the development of cloned embryos derived from these cells was reduced. …”
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    Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos
  8. 1728
    “…The best 3HP producing clone, with 5.45 g.L(-1) of 3HP, produced 11 times more 3HP than the lowest producing clone, which demonstrates the capability of EasyCloneMulti vectors to impact metabolic pathway enzyme activity.…”
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    EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae
  9. 1729
    “…Isolation of peptide-specific T-cell clones is highly desirable for determining the role of T-cells in human disease, as well as for the development of therapies and diagnostics. …”
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    T-cell libraries allow simple parallel generation of multiple peptide-specific human T-cell clones
  10. 1730
    “…A gene encoding F3′H, designated as CsF3′H, was isolated from Camellia sinensis with a homology-based cloning technique and deposited in the GenBank (GenBank ID: KT180309). …”
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    Cloning and Characterization of a Flavonoid 3′-Hydroxylase Gene from Tea Plant (Camellia sinensis)
  11. 1731
    “…In this study, we generated transgenic cloned cows with the marker-free vector pBAC-hLF-hLZ, which was shown to efficiently express rhLZ in cow milk. …”
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    Large-scale production of functional human lysozyme from marker-free transgenic cloned cows
  12. 1732
    “…Therefore, EC-SOD would be an excellent therapeutic drug for treatment of diseases caused by oxidative stress. We cloned both the full length (residues 1–240) and truncated (residues 19–240) forms of human EC-SOD (hEC-SOD) into the donor plasmid pFastBacHTb. …”
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    Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells
  13. 1733
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    Hypervirulent emm59 Clone in Invasive Group A Streptococcus Outbreak, Southwestern United States
  14. 1734
    “…However, clone 1849 antibody inhibited TF‐associated blood coagulation activity. …”
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    Utility of epirubicin‐incorporating micelles tagged with anti‐tissue factor antibody clone with no anticoagulant effect
  15. 1735
    “…To achieve this goal, reverse transcription-polymerase chain reaction (RT-PCR) was performed to clone porcine PTTG1. Subsequently, germinal vesicle (GV)- and metaphase II (MII)-stage oocytes, zygotes, 2-, 4-, and 8-cell-stage embryos, morulas, and blastocysts were produced in vitro and their gene expression was analyzed. …”
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    Cloning of Porcine Pituitary Tumor Transforming Gene 1 and Its Expression in Porcine Oocytes and Embryos
  16. 1736
    “…Here we describe the generation of heterozygous fibrillin-1 (FBN1) mutant cloned pigs (+/Glu433AsnfsX98) using genome editing and somatic cell nuclear transfer technologies. …”
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    Generation of heterozygous fibrillin-1 mutant cloned pigs from genome-edited foetal fibroblasts
  17. 1737
    “…Furthermore, nearly all (>90%) of the TCR sequences that were shared within mice were identical at the DNA sequence level, indicating that conventional and regulatory T cells that express shared TCRs are derived from common clones. Analysis of TCR repertoire overlap in the thymus reveals that a large proportion of Tconv and Treg sharing observed in the periphery is due to clonal expansion in the thymus. …”
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    Conventional and Regulatory CD4(+) T Cells That Share Identical TCRs Are Derived from Common Clones
  18. 1738
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    Cloning, expression and characterization of a β-d-xylosidase from Lactobacillus rossiae DSM 15814(T)
  19. 1739
    “…Surprisingly, both control and A3B-eGFP clones also elicited strong mutator phenotypes characteristic of defective mismatch repair. …”
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    Mutation Processes in 293-Based Clones Overexpressing the DNA Cytosine Deaminase APOBEC3B
  20. 1740
    “…Our phylogenetic analyses of 16S rDNA and endomannanase sequences revealed that T. alba CECT3323 is miss-classified; it belongs to the T. fusca species. The cloned and investigated endomannanases belong to the family of glycosyl hydrolases 5 (GH5), their size is around 50 kDa and they are modular enzymes. …”
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    Cloning, Expression and Biochemical Characterization of Endomannanases from Thermobifida Species Isolated from Different Niches
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