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  1. 1741
    “…The dnak gene from one representative, Bacillus pumilus strain B3 isolated from Bakreshwar hot springs was successfully cloned and sequenced. The dnaK gene was flanked by gene grpE on one side. …”
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    Cloning and expression of dnaK gene from Bacillus pumilus of hot water spring origin()
  2. 1742
    “…OBJECTIVES: The aim of this study is the cloning, expression, and purification of recombinant human EGF (rhEGF), and an assessment of its activity. …”
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    Cloning, Expression, and Cost Effective Purification of Authentic Human Epidermal Growth Factor With High Activity
  3. 1743
    por Libbey, Jane E., Fujinami, Robert S.
    Publicado 2016
    “…The murine pneumotropic virus genome encoded by the pKV(37-1) clone was sequenced to completion. The regulatory region harbored a mutation not previously reported. …”
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    Complete Genome Sequence of Murine Pneumotropic Virus (Polyomaviridae) Clone pKV(37-1)
  4. 1744
    “…Here we isolated a small heat shock protein gene (MsHSP17.7) from alfalfa by homology-based cloning. MsHSP17.7 contains a 477-bp open reading frame and encodes a protein of 17.70-kDa. …”
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    Molecular cloning and characterization of the MsHSP17.7 gene from Medicago sativa L.
  5. 1745
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    Molecular cloning, expression analysis and subcellular localization of four DELLA genes from hybrid poplar
  6. 1746
    “…To characterize VPEs and the relationship between stenospermocarpic grapes and the VPE gene family, we identified 3 Vitis vinifera VPE genes (VvβVPE, VvγVPE, and VvδVPE) from the PN40024 grape genome and cloned the full-length complementary DNAs (cDNAs) from the ‘Vitis vinifera cv. …”
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    Gene Cloning, Expression and Enzyme Activity of Vitis vinifera Vacuolar Processing Enzymes (VvVPEs)
  7. 1747
    “…CONCLUSIONS: Our results suggest that the HCC and ICC components of CHC may originate from the same clone, having the potential for dual-directional differentiation similar to HPCs. …”
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    Combined hepatocellular and cholangiocarcinoma originating from the same clone: a pathomolecular evidence-based study
  8. 1748
    “…In the present study, cloning and characterization of S. miltiorrhiza Bunge squalene synthase 2 (SmSQS2, Genbank Accession Number: KM408605) cDNA was investigated subsequently followed by its recombinant expression and preliminary enzyme activity. …”
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    Molecular Cloning and Functional Analysis of Squalene Synthase 2(SQS2) in Salvia miltiorrhiza Bunge
  9. 1749
    “…For EV-A71, such tools are limited to clones that are transcriptionally controlled by T7/SP6 bacteriophage promoter. …”
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    Enterovirus A71 DNA-Launched Infectious Clone as a Robust Reverse Genetic Tool
  10. 1750
    “…The phage ST79 endolysin gene identified as peptidase M15A was cloned, expressed and purified to evaluate its potential to lyse pathogenic bacteria. …”
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    Cloning, expression, and characterization of a peptidoglycan hydrolase from the Burkholderia pseudomallei phage ST79
  11. 1751
    “…During the cloning procedure, we found a new testis-specific alternative splicing product of the DGKη gene, DGKη4, which lacks half of the catalytic domain. …”
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    Cloning and Characterization of Novel Testis-Specific Diacylglycerol Kinase η Splice Variants 3 and 4
  12. 1752
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    Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo
  13. 1753
    “…RESULTS: In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. …”
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    Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis
  14. 1754
    “…By screening enzyme activities of extracts derived from a yeast knock-out collection, we were able to identify and clone the yeast gene (GPC1) encoding the enzyme, named glycerophosphocholine acyltransferase (GPCAT). …”
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    Cloning of Glycerophosphocholine Acyltransferase (GPCAT) from Fungi and Plants: A NOVEL ENZYME IN PHOSPHATIDYLCHOLINE SYNTHESIS
  15. 1755
    “…We identify chromosome alterations 13q14−, 6q− and 12q+ in early cell clones, elimination of clonal populations following therapy, and subsequent appearance of a clone containing trisomy 12 and chromosome 10 copy-neutral loss of heterogeneity that marks a major population dominant at death. …”
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    Evolution of multiple cell clones over a 29-year period of a CLL patient
  16. 1756
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    Molecular Cloning and Functional Analysis of a Na(+)-Insensitive K(+) Transporter of Capsicum chinense Jacq
  17. 1757
    “…However, little is known about whether micro-injected endosymbionts can have any effects on aphid clones, which was measured using various ecological parameters. …”
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    Ecological impact of a secondary bacterial symbiont on the clones of Sitobion avenae (Fabricius) (Hemiptera: Aphididae)
  18. 1758
    “…Vipp1 is conserved in photosynthetic organisms and forms a high homo-oligomer complex structure that may help sustain the membrane integrity of chloroplasts. This study cloned two novel VIPP1 genes from Triticum urartu and named them TuVipp1 and TuVipp2. …”
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    Two Novel Vesicle-Inducing Proteins in Plastids 1 Genes Cloned and Characterized in Triticum urartu
  19. 1759
    “…Here, we characterize 32 isolates of a new CG-MRSA clone. These isolates were identified in four cities in Colombia, South America. …”
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    Emergence and spread of a new community-genotype methicillin-resistant Staphylococcus aureus clone in Colombia
  20. 1760
    “…In this article, we describe application of an infectious clone system for DENV serotype 1 (DENV1). Similar to previous clones in both flaviviruses and coronaviruses, the approach constructs a panel of contiguous cDNAs that span the DENV genome and can be systematically and directionally assembled to produce viable, full-length viruses. …”
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    Epitope Addition and Ablation via Manipulation of a Dengue Virus Serotype 1 Infectious Clone
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