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  1. 1801
    “…We investigated disease resistance in Vitis amurensis to identify PRRs that are important for resistance against downy mildew, analyzed the PRRs that were upregulated by incompatible Plasmopara viticola infection, and cloned the full-length cDNA of the VaHAESA gene. We then analyzed the structure, subcellular localization, and relative disease resistance of VaHAESA. …”
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    Cloning, Characterization, and Functional Investigation of VaHAESA from Vitis amurensis Inoculated with Plasmopara viticola
  2. 1802
    “…In the procedures published to date, the cloning step that introduces a specific sgRNA target sequence into the plasmid is the most tedious and time-consuming. …”
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    A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast
  3. 1803
    “…We cloned, expressed, purified, and determined the kinetic constants of the recombinant α-carbonic anhydrase (rec-MgaCA) identified in the mantle tissue of the bivalve Mediterranean mussel, Mytilus galloprovincialis. …”
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    Cloning, expression and purification of the α-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis
  4. 1804
    “…WGS provided enhanced understanding of the distribution of antimicrobial resistance clones, including replacement with clones that were more susceptible to antimicrobials, in several risk groups nationally and regionally. …”
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    Public health surveillance of multidrug-resistant clones of Neisseria gonorrhoeae in Europe: a genomic survey
  5. 1805
    “…It emerged as a global pathogen due to the successful expansion of a few epidemic lineages, or international clones (ICs), producing acquired class D carbapenemases (OXA-type). …”
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    Emergence and spread of carbapenem-resistant Acinetobacter baumannii international clones II and III in Lima, Peru
  6. 1806
    “…Some patients with acute myeloid leukemia (AML) who are in complete remission after induction chemotherapy harbor persisting pre-leukemic clones, carrying a subset of leukemia-associated somatic mutations. …”
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    Persistence of pre-leukemic clones during first remission and risk of relapse in acute myeloid leukemia
  7. 1807
    “…Understanding the underlying molecular mechanisms requires reverse genetic systems, which allow manipulation of infectious cDNA clones at will. In the case of flaviviruses, to which ZIKV belongs, several reports have indicated that the construction of full-length cDNA clones is difficult due to toxicity during plasmid amplification in Escherichia coli. …”
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    A Reverse Genetics System for Zika Virus Based on a Simple Molecular Cloning Strategy
  8. 1808
    “…Each colony is split and sequentially exported for on-target sequencing and further off-chip clonal expansion of the validated clones. Using this method, single-clone editing efficiencies, including the rate of mono- and bi-allelic indels or precise nucleotide replacements, can be assessed within 10 days from Cas9 ribonucleoprotein introduction in cells.…”
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    Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device
  9. 1809
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    Molecular Cloning and Characterization of Carotenoid Pathway Genes and Carotenoid Content in Ixeris dentata var. albiflora
  10. 1810
    “…This novel, efficient RNAi and auto-cloning Cas9 systems can be easily adapted to any other genetic model.…”
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    Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research
  11. 1811
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    Molecular Cloning and Functional Characterization of the Dehydrin (IpDHN) Gene From Ipomoea pes-caprae
  12. 1812
    “…NL15-2K as a biocatalyst for vanillin production, it is necessary to characterize the vanillin dehydrogenase (VDH) that degrades the produced vanillin to vanillic acid, as well as the gene encoding this enzyme. Here, we cloned the VDH-encoding gene (vdh) from strain NL15-2K and comprehensively characterized its gene product. …”
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    Molecular cloning and characterization of vanillin dehydrogenase from Streptomyces sp. NL15-2K
  13. 1813
    “…BACKGROUND: A large outbreak with different clones of vancomycin-resistant enterococci (VRE) affected the Bern University Hospital group for several months. …”
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    1255. First Outbreak Due to Vancomycin-Resistant Enterococcus Epidemic Clone ST796 in Europe
  14. 1814
    “…In this study, a gene encoding an endoglucanase was cloned from red rice crop residues using a metagenomic strategy. …”
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    A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues
  15. 1815
    “…African clones using WGS-imputed markers. Optimized TPs gave higher prediction accuracies for CBSD3s and CBSD6s than random TPs of the same size. …”
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    Training Population Optimization for Prediction of Cassava Brown Streak Disease Resistance in West African Clones
  16. 1816
    “…By using caged primers in PCR, unnatural sticky-ends of any sequence, which are sufficiently long for ligation-independent cloning (LIC), are directly prepared on the product after a brief UVA irradiation. …”
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    Enzyme Treatment-Free and Ligation-Independent Cloning Using Caged Primers in Polymerase Chain Reactions
  17. 1817
    “…Here we developed a statistical framework to detect TCR clone proliferation and contraction from longitudinal repertoire data. …”
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    Precise tracking of vaccine-responding T cell clones reveals convergent and personalized response in identical twins
  18. 1818
    “…CONCLUSIONS: Based on the diversity of JA clones, the present study has not only rapidly and precisely examined the biomass composition and digestibility with MPA-optimized NIRS models but has also selected the ideal JA clones according to FQS. …”
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    An NIRS-based assay of chemical composition and biomass digestibility for rapid selection of Jerusalem artichoke clones
  19. 1819
    “…The PPE17 (Rv1168c) gene was PCR amplified and inserted into pET-21b(+) vector, cloned in E. coli TOP10, and finally expressed in E. coli BL21(DE3). …”
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    Cloning, Expression, and Refolding of PPE17 Protein of Mycobacterium Tuberculosis as a Promising Vaccine Candidate
  20. 1820
    “…However, difficulties in cloning and heterologous overexpression might be particular obstacles of the PDR family, since cloning and overexpression of White Brown Complex 1, a half-size transporter of the same ABCG subfamily with comparable domain organization, was more easily achieved. …”
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    Cloning and expression of selected ABC transporters from the Arabidopsis thaliana ABCG family in Pichia pastoris
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