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  1. 1841
    “…Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escherichia coli. …”
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    Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01
  2. 1842
    “…Our findings will be useful for the development of effective strategies for the prevention and treatment of listeriosis caused by this prevalent clone.…”
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    Genomic dissection of the most prevalent Listeria monocytogenes clone, sequence type ST87, in China
  3. 1843
    “…A new GAG lyase from Microbacterium sp. H14 was cloned, expressed, purified, and characterized, with a molecular weight of approximately 85.9 kDa. …”
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    Cloning, Expression, and Characterization of a New Glycosaminoglycan Lyase from Microbacterium sp. H14
  4. 1844
    “…To construct expression clones for the CRISPR/Cas9, most current methods depend on traditional cloning using Gateway reaction or specific type IIS restriction enzymes and DNA ligation, based on multiple steps of PCR. …”
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    A simple and efficient cloning system for CRISPR/Cas9-mediated genome editing in rice
  5. 1845
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    Compensatory mutations modulate the competitiveness and dynamics of plasmid-mediated colistin resistance in Escherichia coli clones
  6. 1846
    “…CONCLUSIONS: The RPGPs cloning strategy serves as a general solution to avoid plasmid RecA-independent recombination. …”
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    Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
  7. 1847
    “…Genomic RNA of F15 strain bovine enteric coronavirus (BECV) was cloned inE. coli. Three clones (174, 160, PG 78), selected in the cDNA library, including a large portion of the nucleocapsid (N), matrix (M) and peplomeric (S) protein genes, were used as probes for a slot blot hybridization assay. …”
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    Radioactive and enzymatic cloned cDNA probes for bovine enteric coronavirus detection by molecular hybridization
  8. 1848
    “…Here we describe the cloning and expression of IFNγ from the Australian flying fox, Pteropus alecto and the generation of mouse monoclonal and chicken egg yolk antibodies specific to bat IFNγ. …”
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    Cloning, expression and antiviral activity of IFNγ from the Australian fruit bat, Pteropus alecto
  9. 1849
    por Yeh, Hung-Yueh, Klesius, Phillip H.
    Publicado 2009
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    Channel catfish, Ictalurus punctatus, cysteine proteinases: Cloning, characterisation and expression of cathepsin H and L
  10. 1850
    “…Little is known about giant panda IFN-α genes and the role they may play in giant panda immunological responses to viruses. We have cloned genes encoding 12 giant panda IFN-α (AmIFN-α or AmIFNA) subtypes that share from 90 to 99% amino acid sequence identity. …”
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    Gene cloning, sequencing, expression and biological activity of giant panda (Ailuropoda melanoleuca) interferon-α
  11. 1851
    “…Among many possible applications of the PRRSV infectious cDNA clones, development of genetically modified vaccines is of particular interest. …”
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    Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and their potential as vaccine vectors
  12. 1852
    “…The data revealed that only cDNA clones obtained after long distance RT-PCR were infectious. …”
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    Construction of an infectious cDNA clone of Enterovirus 71: Insights into the factors ensuring experimental success
  13. 1853
    “…We investigated whether infectious virus particles could be generated by expression of the full-length cloned genome from the modified baculovirus vector. …”
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    Baculovirus expression of cloned porcine arterivirus generates infectious particles in both insect and mammalian cells
  14. 1854
    “…From these libraries clones were selected to form a linear map across the entire gene A, the putative viral polymerase gene. …”
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    Molecular cloning of the gene encoding the putative polymerase of mouse hepatitis coronavirus, strain A59
  15. 1855
    Publicado 1991
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    AIDS recombinant vaccine; recombinant HIV virus core protein cloning in vaccinia virus vector
  16. 1856
    “…The gene encoding the spike (S) protein from two geographically distinct strains (American and British) of canine coronavirus (CCV) was cloned and sequenced. The nucleotide sequence revealed open reading frames of 1443 or 1453 amino acids, respectively. …”
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    Cloning, sequencing and expression of the S protein gene from two geographically distinct strains of canine coronavirus()
  17. 1857
    “…The coding sequences (CDS) of two lysine demethylases (KDMs), KDM1A and KDM4B, were cloned by RT-PCR. The levels of KDM1A and KDM4B in yaks and cattle–yaks testes were detected using Real-time PCR and Western blotting for mRNA and protein, respectively. …”
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    Cloning and Expression Analysis of Two Kdm Lysine Demethylases in the Testes of Mature Yaks and Their Sterile Hybrids
  18. 1858
    “…METHODS: In this study, the pMELK coding sequence was cloned from swine spleen and characterized. We also quantitatively determined the expression of MELK in 11 tissues isolated from a piglet and determined its subcellular localization when expressed in swine umbilical vein endothelial cells (SUVEC) as a fusion protein. …”
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    Cloning, tissue distribution, expression pattern, and function of porcine maternal embryonic leucine zipper kinase
  19. 1859
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    Effects of Rhizome Integration on the Water Physiology of Phyllostachys edulis Clones under Heterogeneous Water Stress
  20. 1860
    “…The first full-length infectious cDNA clone of equine arteritis virus (EAV) was developed in 1996 and was also the first full-length infectious cDNA clone constructed from a member of the order Nidovirales. …”
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    Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained
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