“…Economic processes rely on cultivation media supporting rapid growth in the exponential phase and high protein production in the stationary phase at minimal media consumption rates. To develop clone‐specific, high‐performing perfusion media we present a straightforward and rapid two‐step approach combining commercially available basal media and feed supplements using design‐of‐experiment. …”
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“…Described microscale cultivation strategy can be used for rapid high-throughput screening of P. pastoris clones, media optimization, and high-throughput recombinant protein production. …”
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“…RESULTS: In the study, five MAPKK (MKK) members were identified in grapevine (cultivar ‘Pinot Noir’), cloned and designated as VvMKK1-VvMKK5. A phylogenetic analysis grouped them into four sub-families based on the similarity of their conserved motifs and gene structure to Arabidopsis MAPKK members. qRT-PCR results indicated that the expression of VvMKK1, VvMKK2, VvMKK4, and VvMKK5 were up-regulated in mature leaf and young blades, and roots, but exhibited low expression in leaf petioles. …”
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“…In this paper, we propose an extended version of multi-level replica detection technique built on Danger Theory (DT), which utilizes a hybrid approach (centralized and distributed) to shield the mobile wireless sensor networks (MWSNs) from clone attacks. The danger theory concept depends on a multi-level of detections; first stage (highlights the danger zone (DZ) by checking the abnormal behavior of mobile nodes), second stage (battery check and random number) and third stage (inform about replica to other networks). …”
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“…To streamline and upscale cloning of custom libraries, we present CLUE, a bioinformatic and wet-lab pipeline for the multiplexed generation of pooled sgRNA libraries. …”
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“…To reach this goal we set up a greenhouse experiment cultivating in pot an elite black poplar clone (N12) selected in the past for its excellent ability to reclaim heavy metals. …”
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“…Using the forward genetic screening and subsequent whole-genome resequencing approach, we cloned the MNP1 gene and found that it encodes a putative copalyl diphosphate synthase (CPS) implicated in the first step of gibberellin (GA) biosynthesis. …”
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“…CONCLUSION: To our knowledge, this is the earliest study report to gene cloning and identification of the chitinase gene in Myxococcus fulvus. …”
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“…To facilitate preclinical testing of T-cell receptors (TCRs) derived from tumor-reactive T-cell clones it is necessary to develop convenient and rapid cloning strategies for the generation of TCR expression constructs. …”
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“…CONCLUSION: These results revealed the diversity in the genetic backgrounds of MRSA isolates and the stable maintenance of the dominant MRSA clones in Kuwait hospitals in 2016 and 2017 suggesting an on-going transmission of these clones. …”
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“…To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. …”
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“…In this article, we present Golden Gate cloning-compatible modular systems allowing rapid and flexible construction of polycistronic expression vectors applicable for plants. …”
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“…RESULTS: We describe PyClone-VI, a computationally efficient Bayesian statistical method for inferring the clonal population structure of cancers. …”
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“…RESULTS: In this study, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant K. obovata. The KoOsmotin protein was composed of 221 amino acids and showed a calculated molecular mass of 24.11 kDa with pI 4.92. …”
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“…The interaction of site × clone effects was not significant. The results showed that for short rotation forestry (SRF), the site parameters—especially groundwater availability—are key factors.…”
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