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  1. 1881
    “…Taken together, the development of Golden Gate cloning protocols to build combinatorial pathway libraries, and the optimization of culture conditions set a new stage for accessing the violacein pathway intermediates and engineering violacein production in Y. lipolytica. …”
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    A Golden-Gate Based Cloning Toolkit to Build Violacein Pathway Libraries in Yarrowia lipolytica
  2. 1882
    “…In tumour-adjacent epithelia, clone sizes are smaller than in the background epithelia, as a whole. …”
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    Heterogeneity in clone dynamics within and adjacent to intestinal tumours identified by Dre-mediated lineage tracing
  3. 1883
    “…To address this lacuna, we previously applied transformation-associated recombination (TAR) cloning to isolate individual rDNA units from chromosome 21. …”
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    The genomic structure of a human chromosome 22 nucleolar organizer region determined by TAR cloning
  4. 1884
    “…Direct cloning represents the most efficient strategy to access the vast number of uncharacterized natural product biosynthetic gene clusters (BGCs) for the discovery of novel bioactive compounds. …”
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    Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
  5. 1885
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    Adiponectin Improves In Vitro Development of Cloned Porcine Embryos by Reducing Endoplasmic Reticulum Stress and Apoptosis
  6. 1886
    “…Thus, NKG2C expression in highly differentiated CD56(+) T cells was associated with the most expanded αβ T cell clones. NKG2C(+) T cells produced almost no IFN-γ in response to stimulation with HCMV pp65-derived peptides. …”
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    Surface NKG2C Identifies Differentiated αβT-Cell Clones Expanded in Peripheral Blood
  7. 1887
    “…However, it does not give information on the functional phenotype of the responding clones and lacks a statistical framework for quantitative evaluation. …”
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    Characterization and Monitoring of Antigen-Responsive T Cell Clones Using T Cell Receptor Gene Expression Analysis
  8. 1888
    “…Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. …”
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    Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle
  9. 1889
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    From Clones to Buds and Branches: The Use of Lung Organoids to Model Branching Morphogenesis Ex Vivo
  10. 1890
    por Samiec, Marcin, Skrzyszowska, Maria
    Publicado 2021
    “…This accelerates the processes of epigenomic aging and replicative senescence in the cells forming various tissues and organs of cloned fetuses and progeny. For all the above-mentioned reasons, the current paper aims to overview the state of the art in not only molecular mechanisms underlying intergenomic communication between nuclear and mtDNA molecules in cloned embryos but also intrinsic determinants affecting unfaithful epigenetic reprogrammability of telomeres. …”
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    Extranuclear Inheritance of Mitochondrial Genome and Epigenetic Reprogrammability of Chromosomal Telomeres in Somatic Cell Cloning of Mammals
  11. 1891
    “…In this study, the UAP gene from C. medinalis (CmUAP) was cloned and characterized. The cDNA of CmUAP is 1788 bp in length, containing an open reading frame of 1464 nucleotides that encodes 487 amino acids. …”
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    Cloning, Characterization, and RNA Interference Effect of the UDP-N-Acetylglucosamine Pyrophosphorylase Gene in Cnaphalocrocis medinalis
  12. 1892
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    Correction: Quantitative distribution of patient-derived leukemia clones in murine xenografts revealed by cellular barcodes
  13. 1893
    “…Therefore, we investigated the seasonal biochemical responses of Dendrocalamus strictus clones viz. Pantnagar (PNT) and Dhampur (DHM) to seasonal ambient atmospheric conditions. …”
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    Seasonal variation in biochemical responses of bamboo clones in the sub-tropical climate of Indian Himalayan foothills
  14. 1894
    “…Methods: We tested five different PD-L1 clones (SP263, SP142, E1L3N, 22-8, 22C3) on primary HNSCC tumor tissue of 75 patients in the form of tissue microarrays. …”
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    Performance of Different Diagnostic PD-L1 Clones in Head and Neck Squamous Cell Carcinoma
  15. 1895
    “…Previously, we had developed a vector toolkit EasyClone-MarkerFree for stable integration into eleven sites on chromosomes X, XI, and XII of S. cerevisiae. …”
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    Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites
  16. 1896
    “…CONCLUSIONS: The nuclear transfer cloning, being an ideal tool to generate cloned embryos, and the embryonic stem cells will boost drug testing and cellular medicine in COVID-19.…”
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    Stem cell therapies and benefaction of somatic cell nuclear transfer cloning in COVID-19 era
  17. 1897
    “…This project focuses on a seldom-investigated identity attack—the Clone ID attack—directed at the Routing Protocol for Low Power and Lossy Networks (RPL), the underlying technology for most IoT devices. …”
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    A Dense Neural Network Approach for Detecting Clone ID Attacks on the RPL Protocol of the IoT
  18. 1898
    “…CLoNe additionally saves clusters as individual trajectories for further analysis and provides scripts for automated use with molecular visualization software. …”
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    CLoNe: automated clustering based on local density neighborhoods for application to biomolecular structural ensembles
  19. 1899
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    Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection
  20. 1900
    “…The esxA was successfully cloned from the NK3-4 genome and ligated to the eukaryotic expression vector pPICZαA. …”
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    Cloning and expression of the EsxA gene and the growth-promoting effects of the encoded protein on rice seedlings
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