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  1. 1921
    “…Here, we report the establishment of deltavirus infectious clones with 1.2× genome inserts bearing two copies of the genomic and antigenomic ribozymes. …”
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    Short ‘1.2× Genome’ Infectious Clone Initiates Kolmiovirid Replication in Boa constrictor Cells
  2. 1922
    “…When all clones detected on days 1-3 were considered as true complexity of infection, sampling on day 1 detected only 69.4% (109/157) or 68.3% (228/334) of all clones in incident and chronic infections, respectively. …”
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    Improved Detectability of Plasmodium falciparum Clones with Repeated Sampling in Incident and Chronic Infections in Burkina Faso
  3. 1923
    “…Recently the MLIV gene, MCOLN1, has been identified as a new member of the transient receptor potential (TRP) cation channel superfamily. Here we report the cloning and characterization of the mouse homologue, Mcoln1, and report a novel splice variant that is not seen in humans. …”
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    Cloning and characterization of the mouse Mcoln1 gene reveals an alternatively spliced transcript not seen in humans
  4. 1924
    “…Surveillance programs help monitor and promptly identify the emergence of new clones. We reported the rapid spread of a novel clone of K. pneumoniae co-harbouring class A and D carbapenemases in colonized patients, and the potential risk factors involved in the development of infections. …”
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    High-Risk Clone of Klebsiella pneumoniae Co-Harbouring Class A and D Carbapenemases in Italy
  5. 1925
    “…Both G strain and clone D11 belong to the T. cruzi lineage TcI. Here, we designed intraspecific array-based comparative genomic hybridization (aCGH) to identify chromosomal regions harboring copy-number variations between clone D11 and the G strain. …”
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    Trypanosoma cruzi Genomic Variability: Array Comparative Genomic Hybridization Analysis of Clone and Parental Strain
  6. 1926
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    Reannotation of Fly Amanita l-DOPA Dioxygenase Gene Enables Its Cloning and Heterologous Expression
  7. 1927
    “…Based on genome mining, we discovered nine transcriptionally active genes encoding polyketide synthases (PKSs), Preu1–Preu9, of which those of Preu3, Preu4, and Preu6 were cloned and functionally characterized due to possessing complete sets of synthetic and release domains. …”
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    Cloning and Functional Characterization of the Polyketide Synthases Based on Genome Mining of Preussia isomera XL-1326
  8. 1928
    “…In this study, the CmRAX2 gene cloned by homologous cloning in Chrysanthemum morifolium ‘Jinba’ is localized in the nucleus and cytoplasm, having a complete open reading frame (ORF) of 1050 bp and encoding 350 amino acids. …”
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    Cloning and Functional Verification of CmRAX2 Gene Associated with Chrysanthemum Lateral Branches Development
  9. 1929
    “…Here, we report the cloning of the DnMSI1 gene from Dendrobium nobile Lindl with RACE technology. …”
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    Cloning and expression analysis of DnMSI1 gene in orchid species Dendrobium nobile Lindl
  10. 1930
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    Tissue Expression Analysis, Cloning, and Characterization of the 5′-Regulatory Region of the Bovine LATS1 Gene
  11. 1931
    “…Streptomyces strains with glucose oxidase gene were detected by PCR, and the amplified gene fragment was cloned into Escherichia coli host bacterium using TA cloning technique. …”
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    Molecular Cloning, Expression, and Enzyme Activity of Glucose Oxidase Gene from Soil Thermophilic Streptomyces
  12. 1932
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    Increased expression of pro‐inflammatory cytokines at the fetal–maternal interface in bovine pregnancies produced by cloning
  13. 1933
    “…Here, we describe a protocol to prepare stocks of an infectious clone of SARS-CoV-2 expressing a stable mNeonGreen reporter (icSARS-CoV-2-mNG). …”
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    Infecting kidney organoids with a cDNA reporter clone of SARS-CoV-2
  14. 1934
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    Fine mapping and cloning of the major seed protein quantitative trait loci on soybean chromosome 20
  15. 1935
    “…In this study, we genotyped a diversity panel of 214 advanced clones with the 22 K SNP potato array and phenotyped it in three field environments in Texas. …”
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    Genomic regions associated with tuber traits in tetraploid potatoes and identification of superior clones for breeding purposes
  16. 1936
    “…Average chondrocyte density increased 1 week after fracture (from 774 to 856 cells/mm(2)), decreased substantially 3 months after fracture (610 cells/mm(2)), and slowly increased 6 and 12 months after fracture (638 and 683 cells/mm(2), respectively). Average detected clone density 3, 6, and 12 months after fracture (11, 11, 9 clones/mm(2)) was higher than the 4–5 clones/mm(2) detected in normal tissue or 1 week after fracture and show local increases in clone density that varied across the joint surface with time. …”
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    Objective evaluation of chondrocyte density & cloning after joint injury using convolutional neural networks
  17. 1937
    “…Our study reveals that abnormal imprinting of the DLK1-DIO3 region occurs in cloned pigs, which provides a theoretical basis for improving the cloning efficiency by gene editing to correct abnormal imprinting.…”
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    Identification of the porcine IG-DMR and abnormal imprinting of DLK1-DIO3 in cloned pigs
  18. 1938
    “…The coding sequence (CDS) of yak HAT1 and HDAC1 were obtained by conventional polymerase chain reaction (PCR) and gene cloning. The testicular mRNA and protein levels of HAT1 and HDAC1 in yaks and cattle-yaks were detected by quantitative PCR (qPCR) and Western blotting, respectively, and the histone H3 lysine 9 (H3K9) histone acetylation level in the testes of yaks and cattle-yaks was assayed using enzyme linked immunosorbent assay (ELISA). …”
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    Cloning and Expression Analysis of HAT1 and HDAC1 in the Testes of Mature Yaks and Their Sterile Hybrids
  19. 1939
    “…The present study reports the gene cloning of metalloproteinase enzymes from jellyfish species for the first time. …”
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    Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish Nemopilema nomurai (Scyphozoa: Rhizostomeae)
  20. 1940
    “…However, capturing signal transduction events in coexisting clones remains challenging from a technical perspective. …”
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    Analysis of neuroendocrine clones in NSCLCs using an immuno-guided laser-capture microdissection-based approach
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