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37881por Ruiz-Masó, José Á., Luengo, Luis M., Moreno-Córdoba, Inmaculada, Díaz-Orejas, Ramón, del Solar, Gloria“…Here, we have studied the role of the regulatory circuit mediated by these transcriptional repressors during the establishment of these two plasmids in a new host cell, and found that excess Cop repressor molecules in the recipient cell result in a severe decrease in the frequency and/or the velocity of appearance of transformant colonies for the cognate plasmid but not for unrelated plasmids. …”
Publicado 2017
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37882por Gerits, Evelien, Spincemaille, Pieter, De Cremer, Kaat, De Brucker, Katrijn, Beullens, Serge, Thevissen, Karin, Cammue, Bruno P.A., Vandamme, Katleen, Fauvart, Maarten, Verstraeten, Natalie, Michiels, Jan“…The effect of AM404 on P. gingivalis biofilms formed on titanium disks as a model for dental implants was evaluated by colony forming unit counting. Potential cytotoxicity of AM404 towards HEK‐293 (human embryonic kidney cells), HepG2 (human hepatoma cells), IEC‐6 (rat intestinal cells), and Panc‐1 cells (pancreatic cancer cells) was assessed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assays. …”
Publicado 2017
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37883por Herten, Monika, Bisdas, Theodosios, Knaack, Dennis, Becker, Karsten, Osada, Nani, Torsello, Giovanni B., Idelevich, Evgeny A.“…After incubation for 4 and 18 h, the biofilm was determined by three different methods: (a) mitochondrial ATP concentration as measure of bacterial viability (ATP), (b) crystal violet staining (Cry), and (c) vital cell count by calculation of colony-forming units (CFU). The experiments were performed three times. …”
Publicado 2017
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37884por Szulc-Dąbrowska, Lidia, Struzik, Justyna, Cymerys, Joanna, Winnicka, Anna, Nowak, Zuzanna, Toka, Felix N., Gieryńska, Małgorzata“…Our results showed that in vitro infection of granulocyte-macrophage colony-stimulating factor-derived bone marrow cells (GM-BM—comprised of cDCs and macrophages) from C57BL/6 and BALB/c mice similarly down-regulated multiple genes engaged in DC innate and adaptive immune functions, including antigen uptake, processing and presentation, chemokines and cytokines synthesis, and signal transduction. …”
Publicado 2017
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37885por Fujii, Osamu, Tatsumi, Sawako, Ogata, Mao, Arakaki, Tomohiro, Sakaguchi, Haruna, Nomura, Kengo, Miyagawa, Atsumi, Ikuta, Kayo, Hanazaki, Ai, Kaneko, Ichiro, Segawa, Hiroko, Miyamoto, Ken-ichi“…In Experiment 2, we administered granulocyte colony-stimulating factor to mice to disrupt the osteocyte canalicular network. …”
Publicado 2017
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37886“…The effects of miR-25-3p on proliferation, apoptosis capacity of TNBC were evaluated using Cell counting kit-8 (CCK-8), colony formation assay and Annexin V-FITC/PI analyses. …”
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37887“…RESULTS: In vitro, 10(−7) M IP was selected significantly promoting proliferation, ALP activity, colony forming efficiency, and mineral deposition (P<0.05) on hPDLCs. …”
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37888“…Anchorage-independent cell growth was detected by cell Counting Kit-8 (CCK-8) assay and soft agar colony formation assay. Anoikis was detected by ethdium homodimer-1 (EthD-1) staining and flow cytometry analysis. …”
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37889“…OBJECTIVE: Colony-forming units of granulocytes/macrophages (CFU-GM) analysis is the most widely used method to determine the hematopoietic stem cell (HSC) content of human umbilical cord blood (CB) for prediction of engraftment potential. …”
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37890por Kitamura, Takanori, Doughty-Shenton, Dahlia, Cassetta, Luca, Fragkogianni, Stamatina, Brownlie, Demi, Kato, Yu, Carragher, Neil, Pollard, Jeffrey W.“…Expression of these MAM-associated genes in MAMPCs was reduced by genetic deletion of colony-stimulating factor 1 receptor (CSF1R). On the other hand, transient CSF1R blockade did not reduce the number of MAMPCs in the metastatic site, suggesting that CSF1 signaling is active in MAMPCs but is not required for their accumulation. …”
Publicado 2018
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37891por Khurana, Namrata, Kim, Hogyoung, Chandra, Partha K., Talwar, Sudha, Sharma, Pankaj, Abdel-Mageed, Asim B., Sikka, Suresh C., Mondal, Debasis“…MTT cell viability, wound-heal assay, and colony forming unit (CFU) measurements revealed that 22Rv1 cells are resistant to the anti-androgen, enzalutamide (ENZ). …”
Publicado 2017
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37892por Zhang, Dongmei, Tian, Xinlun, Feng, Ruie, Guo, Xiaobei, Wang, Peng, Situ, Yusen, Xiao, Yi, Xu, Kai-Feng“…METHODS: Patients pathologically diagnosed with PAP and with negative results for anti-granulocyte macrophage colony stimulating factor (GM-CSF) autoantibodies from Peking Union Medical College Hospital between January 2000 and July 2016 were retrospectively studied. …”
Publicado 2018
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37893por Zhang, Qiaoge, Song, Ge, Yao, Lili, Liu, Yankun, Liu, Min, Li, Shengping, Tang, Hua“…We studied miR-3928v function in HCC cell lines by MTT, colony formation, migration/invasion, and vascular mimicry (VM) assays in vitro and by a xenograft tumor model in vivo. …”
Publicado 2018
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37894miR‐204‐5p suppresses hepatocellular cancer proliferation by regulating homeoprotein SIX1 expressionpor Chu, Yi, Jiang, Mingzuo, Du, Feng, Chen, Di, Ye, Tao, Xu, Bing, Li, Xiaowei, Wang, Weijie, Qiu, Zhaoyan, Liu, Haiming, Nie, Yongzhan, Liang, Jie, Fan, Daiming“…We demonstrated that ectopic expression of miR‐204‐5p in HCC cell lines inhibited HCC cell proliferation and clonogenicity using CCK8, BrdU and colony‐forming assays, while the inhibition of miR‐204‐5p enhanced proliferation and clonogenicity. …”
Publicado 2018
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37895por Hanazawa, Asami, Ito, Ryoji, Katano, Ikumi, Kawai, Kenji, Goto, Motohito, Suemizu, Hiroshi, Kawakami, Yutaka, Ito, Mamoru, Takahashi, Takeshi“…Engraftment of HSC4 cells, a human head and neck squamous cell carcinoma-derived cell line producing various factors including IL-6, IL-1β, macrophage colony-stimulating factor (M-CSF), and vascular endothelial growth factor (VEGF), into HSC-NOG-hIL-6 Tg mice induced a significant number of TAM-like cells, but few were induced in HSC-NOG non-Tg mice. …”
Publicado 2018
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37896por Bozec, Aline, Luo, Yubin, Engdahl, Cecilia, Figueiredo, Camille, Bang, Holger, Schett, Georg“…METHODS: Peripheral blood CD68(+) monocytes stimulated with macrophage colony-stimulating factor for 24 h were exposed to random immunoglobulin G alone (negative control), purified ACPA, purified RF, or lipopolysaccharide (positive control) in cell culture plates coated with citrullinated vimentin (to allow ACPA immune complex formation). …”
Publicado 2018
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37897por Guay, Isabelle, Boulanger, Simon, Isabelle, Charles, Brouillette, Eric, Chagnon, Félix, Bouarab, Kamal, Marsault, Eric, Malouin, François“…BACKGROUND: Tomatidine (TO) is a plant steroidal alkaloid that possesses an antibacterial activity against the small colony variants (SCVs) of Staphylococcus aureus. We report here the spectrum of activity of TO against other species of the Bacillales and the improved antibacterial activity of a chemically-modified TO derivative (FC04–100) against Listeria monocytogenes and antibiotic multi-resistant S. aureus (MRSA), two notoriously difficult-to-kill microorganisms. …”
Publicado 2018
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37898por Lv, Da-lun, Chen, Lei, Ding, Wei, Zhang, Wei, Wang, He–li, Wang, Shuai, Liu, Wen-bei“…METHODS: Cell viability was examined with cell counting Kit 8 assay and colony formation assay; Apoptosis was evaluated by flow cytometry and Caspase 3/7 activity assay; Western blotting was used to test proteins related to autophagy and the AKT/mammalian target of rapamycin (mTOR) signaling pathway; Tumor xenograft model in BALB/c nude mice was performed to evaluate the effects of SMI-4a and combined SMI-4a with G-Rh2 in anti-melanoma in vivo. …”
Publicado 2018
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37899“…METHODS: The impact of a PX-UVD on surface colony forming units per square centimeter (cfu/cm(2)) in feed preparation areas was evaluated following its implementation as standard care. …”
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37900“…RESULTS: Viability of fungal decreased significantly in CXL group with 30-min irradiation compared with that in control, riboflavin and UVA groups (P < 0.01). The colony-forming units (CFUs) of fungal solutions in culture significantly decreased with CXL treatment (P < 0.05). …”
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