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37901por Zhang, Hao, Li, Zhong-Li, Yang, Fei, Zhang, Qiang, Su, Xiang-Zheng, Li, Ji, Zhang, Ning, Liu, Chun-Hui, Mao, Ning, Zhu, Heng“…To explore the “stemness” of MSCs, cell colony-forming tests and multidifferentiation assays were performed. …”
Publicado 2018
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37902“…After Dex and/or mangiferin treatment, cell viability, apoptosis and reactive oxygen species (ROS) production was measured by Cell Counting kit-8 (CCK-8) and flow cytometry, respectively, and the concentration of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and macrophage colony-stimulating factor (M-CSF) was measured by ELISA. …”
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37903por David, Sheba R, Malek, Nurafiqah, Mahadi, Abdul Hanif, Chakravarthi, Srikumar, Rajabalaya, Rajan“…The in vivo studies showed that the bacterial colonies were fewer at 1 cm (7×10(1) CFU/mL) than at 2 cm (1.3×10(2) CFU/mL) over a 24-hour period. …”
Publicado 2018
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37904“…There are few published data on the effects of housing laying hens at different densities in large furnished cages (FC; a.k.a. enriched colony cages). The objective of this study was to determine the effects of housing laying hens at 2 space allowances (SA) in 2 sizes of FC on measures of production and well-being. …”
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37905por Wang, Mei, Yang, Fang, Qiu, Rong, Zhu, Mengchu, Zhang, Huiling, Xu, Wenrong, Shen, Bo, Zhu, Wei“…The tumor‐promoting phenotype and function of mMSCs were detected by immunofluorescence staining, quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR), cell colony formation assay, transwell migration, and invasion assays. …”
Publicado 2018
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37906por Ren, Jiaqiang, Ward, Dawn, Chen, Steven, Tran, Katherine, Jin, Ping, Sabatino, Marianna, Robey, Pamela G., Stroncek, David F.“…Marrow aspirates from 5 healthy subjects were cultured for 4 passages in 10% HPGF-C18 or 20% FBS and were analyzed for proliferation, colony formation efficiency (CFE), surface marker expression, suppression of mixed lymphocyte reactions (MLRs), global gene and microRNA expression analysis. …”
Publicado 2018
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37907por Sun, Yan, Huang, Yong-Hao, Huang, Feng-Ying, Mei, Wen-Li, Liu, Quan, Wang, Cai-Chun, Lin, Ying-Ying, Huang, Canhua, Li, Yue-Nan, Dai, Hao-Fu, Tan, Guang-Hong“…Methods: Cell growth and proliferation were detected by MTT, lactate dehydrogenase (LDH) release, 5-ethynyl-20-deoxyuridine (EDU) and colony formation assays. Cell apoptosis was detected by flow cytometry. …”
Publicado 2018
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37908por S.M., Fazeela Mahaboob Begum, Chitra, Kalai, Joseph, Benin, Sundararajan, Raji, S., Hemalatha“…The in-vitro studies were performed to check the efficacy of the extract on cell proliferation (MTT assay), cell invasion (scratch assay and colony formation assay), apoptosis (fluorescent, confocal microscopy and flow cytometry) and expression of apoptosis and cell survival proteins including PI3K, Akt and GSK3β and matrix metalloproteinase MMP2 and MMP9 by Western blot method. …”
Publicado 2018
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37909por Al-Dwairi, Ahmed, Alqudah, Tamara E, Al-Shboul, Othman, Alqudah, Mohammad, Mustafa, Ayman G, Alfaqih, Mahmoud A“…However, exendin-4 (50 and 100 nM) significantly (p<0.05) reduced the expression of multiple cytokines including tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α), T cell activation gene-3 (TCA-3), stromal cell-derived factor-1 (SDF-1), and macrophage colony stimulating factor (M-CSF). To confirm these results, expression of these cytokines was further assessed by enzyme-linked immunosorbent assay and real-time polymerase chain reaction and similar results were also observed. …”
Publicado 2018
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37910Ring finger protein 38 promote non-small cell lung cancer progression by endowing cell EMT phenotypepor Xiong, Dian, Zhu, Shu-Qiang, Wu, Yong-Bing, Jin, Chun, Jiang, Jia-Hao, Liao, Yun-Fei, Long, Xiang, Wu, Hai-Bo, Xu, Jian-Jun, Li, Ji-Jun, Ding, Jian-Yong“…Wound-healing assays, trans-well assays, colony formation assays and CCK8 were used to assess cell migration, invasion and proliferative ability respectively. …”
Publicado 2018
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37911por Liu, Wenpeng, Kang, Lei, Han, Juqiang, Wang, Yadong, Shen, Chuan, Yan, Zhifeng, Tai, Yanhong, Zhao, Caiyan“…MATERIALS AND METHODS: Cell proliferation was evaluated by cell counting Kit-8 and colony formation assay. Western blot and real-time PCR were accordingly used to detect the relevant proteins, miRNA and gene expression. …”
Publicado 2018
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37912por Li, Li, Pielsticker, Colin, Han, Zifeng, Kubasová, Tereza, Rychlik, Ivan, Kaspers, Bernd, Rautenschlein, Silke“…In Experiment A significant higher numbers of colony forming units (CFU) of C. jejuni were detected in the caecum of co-inoculated birds compared to C. jejuni-mono-inoculated birds in the early phase after C. jejuni-inoculation. …”
Publicado 2018
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37913“…The expression of HOXA10 was detected by Immunofluorescence staining in endometrial cancer tissues and adjacent normal tissues. CCK-8 and colony formation assays were used to assess the effect of miR-139-5p on ECC1 and Ishikawa cell line proliferation. …”
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37914por Cunha, Mateus Trinconi, Murça, Maria Aparecida, Nigro, Stanley, Klautau, Giselle Burlamaqui, Salles, Mauro José Costa“…Antibacterial activity was evaluated by means of time-kill curve, through seeding the strains on blood agar plates, and subsequently performing colony counts after 24, 48, 72, 96, 120 and 168 h of incubation. …”
Publicado 2018
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37915“…CI increased granulocyte-colony stimulating factor (G-CSF) and keratinocyte chemoattractant (KC) in blood and bone marrow. …”
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37916por Lee, Ming-Chin, Saleh, Reem, Achuthan, Adrian, Fleetwood, Andrew J., Förster, Irmgard, Hamilton, John A., Cook, Andrew D.“…BACKGROUND: Granulocyte macrophage-colony stimulating factor (GM-CSF) has been implicated in the pathogenesis of a number of inflammatory diseases and in osteoarthritis (OA). …”
Publicado 2018
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37917por Wang, Juan, Wang, Handong, Sun, Kangjian, Wang, Xiaoliang, Pan, Hao, Zhu, Jianhong, Ji, Xiangjun, Li, Xiang“…METHODS: A Cell Counting Kit-8 assay and a plate colony formation assay were performed to evaluate cell proliferation. …”
Publicado 2018
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37918por Xin, Jing, Wang, Sijia, Wang, Bing, Wang, Jiazhuang, Wang, Jing, Zhang, Luwei, Xin, Bo, Shen, Lijian, Zhang, Zhenxi, Yao, Cuiping“…The anticancer effect was evaluated by CCK-8 assay and colony formation assay. The delivery efficiency of AlPcS(4) and the binding affinity to serum proteins were determined by fluorescence intensity assay. …”
Publicado 2018
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37919por Lyu, Lei, Huang, Lu-qi, Huang, Tao, Xiang, Wei, Yuan, Jing-dong, Zhang, Chuan-hua“…The effects of GA-TAT on EJ cell viability and proliferation were determined by MTT assay, Edu assay and colony formation assay, respectively. After treated with 1.0 μM GA-TAT for 24 h, the apoptosis rate of EJ cells were detected by Acridine orange/ethidium bromide (AO/EB) assay and flow cytometry assay. …”
Publicado 2018
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37920por Yan, Yue, Bao, Hai-Peng, Li, Chun-Lei, Shi, Qi, Kong, Yan-Hua, Yao, Ting, Li, You-Lin“…The expression content of interleukin (IL)-5, IL-10, chemokine (C–C motif) ligand 5 (CCL5), granulocyte–macrophage colony-stimulating factor (GM-CSF), transforming growth factor beta 1 (TGF-β1), interferon (IFN)-γ, and other cytokines in rat serum and the genes of Eotaxin mRNA, Fas mRNA, FasL mRNA, Fas/FasL and Bcl-2 mRNA in the lung tissues were determined. …”
Publicado 2018
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