“…The midge feeding model has been developed to study infection, replication and disseminations of virus in vivo. A laboratory colony of C. sonorensis, a known competent BTV vector, was fed or injected with BTV variants and propagation in the midge was examined using PCR testing. …”
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“…Following plasmid transfection for overexpression, the proliferation of TNBC cells was determined by CCK8 and colony formation assay. Moreover, xenograft tumor models were canvassed to investigate their effect upon in vivo tumor growth. …”
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“…The biological influence of miR-942 on HCC cell lines was studied using CCK-8 assay, colony formation assay and transwell assay in vitro. …”
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“…Then, Nano-DOX was shown to downregulate tumor-derived granulocyte-colony stimulating factor (G-CSF) and suppresses the induction and tissue filtration of myeloid-derived suppressor cells (MDSCs) that are the principal effectors of cancer-associated systemic immunosuppression. …”
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“…METHODS: Over two years of monitoring, we tracked the movements of 17 juvenile king penguins (Aptenodytes patagonicus, ~ 1 year old) using satellite relay tags from Crozet Archipelago (Southern Indian Ocean), starting when birds left their natal colony for the first time. For comparison we also tagged 6 non-breeding adults, which at that stage, similar to juveniles, are unhampered by reproductive constraints and might roam further than breeders. …”
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“…The broth was streaked on blood agar and MacConkey plates. Colonies consistent with possible Staphylococcus spp. were tested with latex agglutination, and positive isolates were plated on CHROMagar™ MSSA/MRSA. …”
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“…Quantitative surface cultures were sent to an independent microbiology laboratory pre- and 11-weeks post-application to identify total bacterial colony-forming units (CFU). HAI outcomes from treatment and contemporaneous control units were assessed using National Healthcare Safety Network protocols for multidrug-resistant organism bloodstream infections (MDRO-BSI) and Clostridium difficile infections (CDI). …”
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“…METHODS: E. faecalis wild-type strain JH2-2 was utilized in a 48-hour in vitro pharmacodynamic model with a starting inoculum of 10(6) colony-forming units (CFU)/mL. Models were performed in duplicate to triplicate. …”
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“…RESULTS: Median and range of recovered viable colonies are presented below. MEDTA+EtOH was significantly more efficacious compared with control (P = 0.002 for all strains) in completely eradicating all replicates in all 10 strains of C. auris tested. …”
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“…METHODS: Five Candida species (C. albicans, C. auris, C. glabrata, C. parapsilosis, and C. tropicalis) were incubated overnight. Colony suspensions for each species were prepared and adjusted to OD 1.0 at absorbance 0.1. …”
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“…Distinct E. coli strains were resolved by genomic profiling of 10 colonies/sample. Strains underwent molecular lineage identification, VG detection, and comparison with the veteran’s clinical isolate. …”
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“…To determine the effect of infection and antibiotic use, 30 larvae were injected (left proleg) with ~1 × 10(5) colony-forming unit (cfu) of methicillin-resistant Staphylococcus aureus (MRSA) and were randomized 1:1:1 to treatment with vancomycin (20 mg/kg) or a natural antimicrobial (Nigella sativa seed oil, 70 mg/kg; NS oil), or a combination. …”
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“…ATR-FTIR spectra were acquired directly from colonies on 5% blood agar plates. RESULTS: A spectral database containing ATR-FTIR spectra of over 4300 bacterial isolates, encompassing over 70 genera and 190 species, was updated to include spectra of 39 isolates collected in this study and employed in the identification of the other isolates (n = 20). …”
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“…CFU levels were measured by enumeration of bacterial colonies following serial dilution of tissue homogenates. …”
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“…Cefiderocol treatment displayed a superior reduction in biofilm based on colony counts ( >90%; P < 0.0001 vs. untreated control) compared with comparator drugs (50 to 80% reduction). …”
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“…Resulting E. coli-like colonies were isolated and examined further for the presence of colistin-resistant extended-spectrum β-lactamase (ESBL)-producing E. coli with mcr-1. …”
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“…Cell proliferation was detected using MTT and colony formation assays. Dual luciferase reporter assay was applied for confirming the binding site between MEG3 and miR-21, miR-21 and Caspase-8. …”
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“…Lastly, the antibacterial activity of 3D4/31 MΦ cells was evaluated by the colony forming unit assay. RESULTS: ASF upregulated the cell viability and growth rate of 3D4/31 MΦ, with or without PMA activation. …”
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“…Bacterial isolates were characterized based on colony appearance, Gram reaction, culture characteristics, and biochemical tests after inoculating on appropriate culture media. …”
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“…METHODS: WHO tube susceptibility bioassays were used for colony selection and to screen for resistance to the major classes of public health insecticides. …”
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