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38121por Mekuriaw, Wondemeneh, Balkew, Meshesha, Messenger, Louisa A., Yewhalaw, Delenasaw, Woyessa, Adugna, Massebo, Fekadu“…A fully susceptible insectary colony of An. arabiensis was fed on treated and control participants at 1, 4, 7, 10 and 13 days post ivermectin-administration. …”
Publicado 2019
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38122“…Biological roles of RASSF6 were examined using MTT, colony formation assay, Matrigel invasion assay, cell cycle analysis, AnnexinV/PI staining and JC-1 staining. …”
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38123“…Cell proliferation was assessed by CCK8, EdU and colony formation assays, while transwell and wound-healing assays were performed to investigate the cell migration and invasion in vitro. …”
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38124por Grist, Hannah, Daunt, Francis, Wanless, Sarah, Burthe, Sarah J., Newell, Mark A., Harris, Mike P., Reid, Jane M.“…We used intensive winter (non‐breeding season) resightings of colour‐ringed adult European shags (Phalacrocorax aristotelis) from a known breeding colony to identify resident and migrant individuals. …”
Publicado 2017
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38125“…Cell viability and proliferation were determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and colony-formation assay. Cell apoptosis and cycle was detected using flow cytometer. …”
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38126por Foote, Andrew, Simma, David, Khatkar, Mehar, Raadsma, Herman, Guppy, Jarrod, Coman, Greg, Giardina, Erika, Jerry, Dean, Zenger, Kyall, Wade, Nick“…A total of 199 broodstock contributed to the 5,734 progeny that were genotyped with a custom multiplex single nucleotide polymorphism (SNP) panel, and family assignments were cross-referenced using two parentage assignment methods, CERVUS and COLONY. A total of 121 families were detected, with some families contributing up to 11% of progeny at 30 DOC and up to 18% of progeny at harvest. …”
Publicado 2019
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38127“…A bronchofibroscopy was conducted with a fungal culture, showing growth of Cryptococcus laurentii colonies. Amphotericin B was started thereafter but discontinued three days later in favor of fluconazole 400 mg/d due to worsening renal function. …”
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38128por Ren, Hao, Saliu, Eva-Maria, Zentek, Jürgen, Goodarzi Boroojeni, Farshad, Vahjen, Wilfried“…In a first step, a massive sample pool consisting of 7102 broiler-derived colonies from intestinal contents were established and sub-cultured. …”
Publicado 2019
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38129“…The mutant library of GH11 endoxylanase was constructed via DNA shuffling by using the catalytic domain of Bacillus amyloliquefaciens xylanase A (BaxA) and Thermomonospora fusca TF xylanase A (TfxA) as parents. A total of 2,250 colonies were collected and 756 of them were sequenced. …”
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38130por Ohta, Naomi, Norby, Bo, Loneragan, Guy H., Vinasco, Javier, den Bakker, Henk C., Lawhon, Sara D., Norman, Keri N., Scott, Harvey M.“…The purpose of this analysis was to quantify the dynamics of Salmonella using colony counting (via a spiral-plating method) and hydrolysis probe-based qPCR (TaqMan(®) qPCR). …”
Publicado 2019
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38131por Luo, Xiaoqiong, Wei, Jingxi, Yang, Feng-lian, Pang, Xiao-xia, Shi, Feng, Wei, Yu-xia, Liao, Bi-yun, Wang, Jun-li“…HeLa/S and HeLa/DDP cells were interfered with HNF1A-AS1 to determine IC(50), proliferation, colony formation and apoptosis of CC cells. The exosomes were isolated and identified. …”
Publicado 2019
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38132“…The aim of this study was to determine if offering female cat neutering assistance to multi-cat owners significantly improved colony welfare. RESULTS: Ten multicat households with a history of public complaint to the RSPCA were recruited. …”
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38133“…METHODS: RT-qPCR was applied to measure the expression level of circ-ITCH and miR-17-5p in PC cell lines and tissues. CCK-8, colony formation, Brdu incorporation labeling and flow cytometry assays were applied to detect the effects of circ-ITCH and miR-17-5p on proliferation and cell apoptosis. …”
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38134por Cui, Yue, Fan, Yuhua, Zhao, Guangcai, Zhang, Qibing, Bao, Ying, Cui, Yuanri, Ye, Zengjie, Chen, Guoyou, Piao, Xianji, Guo, Fang, Wang, Jinghao, Bai, Yuhua, Yu, Dejun“…Bioinformatics analysis was used to identify the relationship between PSMG3-AS1, miR-143-3p and COL1A1. Colony forming and Cell Counting Kit-8 assays were used to detect cell proliferation. …”
Publicado 2020
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38135“…The regulatory effects of microRNA-222-3p on the proliferation, invasion, and apoptosis of activated B cell-like-type diffuse large B-cell lymphoma cells were analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), colony formation, flow cytometry, and Transwell assay. …”
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38136por Lee, Kyung-Ann, Kim, Kyoung-Woon, Kim, Bo-Mi, Won, Ji-Yeon, Min, Hong Ki, Lee, Dhong Won, Kim, Hae-Rim, Lee, Sang-Heon“…Human peripheral blood monocytes were cultured with macrophage colony-stimulating factor (M-CSF) and IL-26, after which osteoclastogenesis was evaluated by counting the number of tartrate-resistant acid phosphatase-positive multinucleated cells. …”
Publicado 2019
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38137“…Gain- and loss-function approaches were employed in CD34(+) HSCs. Colony-forming units (CFU) and expression of dickkopf-1 (DKK1), a hematopoietic inhibiting factor associated with pathogenesis of AML, were determined in CD34(+) HSCs, as well as the extents of JAK2 and STAT3 phosphorylation and LDOC1 expression. …”
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38138por Liu, Li, Wang, Jing, Sun, Guifeng, Wu, Qiong, Ma, Ji, Zhang, Xin, Huang, Nan, Bian, Zhixuan, Gu, Song, Xu, Min, Yin, Minzhi, Sun, Fenyong, Pan, Qiuhui“…And we clarified the effects of these factors on HB cells using cell proliferation assay, colony formation, apoptotic assay. Then we investigated of methyltransferase-like 13 (METTL3) and its correlation with clinicopathological features and used xenograft experiment to check METTL3 effect in vivo. m(6)A-Seq was used to profiled m(6)A transcriptome-wide in hepatoblastoma tumor tissue and normal tissue. …”
Publicado 2019
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38139“…Human GC cell lines AGS and BGC823 were used as cell models. CCK-8 and colony formation assays were conducted to assess the effect of LOXL1-AS1 on the proliferation of GC cell lines. …”
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38140“…METHODS: Firstly, two bioresorbable membranes (polylactide-based and collagen-based) were dipped into 0.06% CHX and 0.12% CHX, before biofilms (2-species representing periodontal health, 6-species representing a periodontitis) were formed for 2 h and 8 h. Subsequently, colony forming units (cfu) were counted. Secondly, the membranes were treated with CHX and inoculated in bacteria suspension two-time per day for 3 d before cfu were determined. …”
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