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38321por Tur, Carlos, Almenar, David, Benlloch-Navarro, Sandra, Argilés-Herrero, Rafael, Zacarés, Mario, Dalmau, Vicente, Pla, Ignacio“…Of the initial L1 larvae, we recovered 17.2% of male pupae after sex sorting to be sterilized and released on the field, while the rest of the pupae remained available to maintain the rearing colony. The residual percentage of females after sex sorting was on average 0.17%. …”
Publicado 2021
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38322“…The proliferation, migration, invasion and epithelial–mesenchymal transition (EMT) changes of bladder cancer cells after FUT7 siRNA or cDNA transfection were determined by CCK8, colony formation, transwell and Western blot, respectively. …”
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38323por Wei, Xuan, Shi, Juanjuan, Lin, Qianhan, Ma, Xiaoxue, Pang, Yingxin, Mao, Hongluan, Li, Rui, Lu, Wei, Wang, Yu, Liu, Peishu“…Proliferative activity was measured by MTT and colony formation assays. Cell cycle and apoptosis analysis were performed by flow cytometry. …”
Publicado 2021
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38324por Zhao, Chunyan, Zhang, Nan, Cui, Xiaoteng, Zhang, Xinxin, Ren, Yuanyuan, Su, Chao, He, Jinyan, Zhang, Wei, Sun, Xiaoming, Yang, Jie, Gao, Xingjie“…We also utilized two glioma cell lines to conduct the colony formation and wound‐healing assay. RESULTS: GAS2L3 gene was highly expressed in glioma tissues compared to normal brain tissues (p < 0.05). …”
Publicado 2021
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38325por Jiang, Wenna, Qiao, Lu, Han, Yawei, Zhang, Aimin, An, Haohua, Xiao, Jiawei, Ren, Li“…The functions of BCAA concentrations and the effects of activated pancreatic stellate cells (aPSCs) were also evaluated by performing Cell Counting Kit-8, colony formation, and wound healing assays. RESULTS: A total of 1,519 proteins with significantly differential expression were discovered in PDAC and adjacent tissues by using nano-LC-MS/MS. …”
Publicado 2021
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38326por Long, Xianming, Li, Xiaojing, Li, Tao, Yan, Qing, Wen, Lihui, Yang, Xixi, Li, Hui, Sun, Lingyun“…After observing the survival rate, another 50 mice were also randomly divided into five groups, and the mice were sacrificed after 24 h. Bacterial colonies from the blood and peritoneal lavage fluid were counted in a blinded manner. …”
Publicado 2021
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38327por Pengcheng, Zhou, Peng, Gao, Haowen, Fan, Xida, Lin, Yuhua, Lu, Yao, Wang, Mingyan, Zhu, Xiangjun, Fan, zhiwei, Wang, Yewei, Zhang, Lei, Wang“…Furthermore, cell proliferation, migration, and invasion were assessed using CCK-8, Edu staining, colony-forming assay, wound healing assay, and transwell assay in vitro. …”
Publicado 2021
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38328“…Wound healing assay and transwell migration assay were performed to assess cell migration ability, whereas cell invasion ability was evaluated by transwell invasion assay. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and colony formation assay were utilized to analyze cell proliferation ability. …”
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38329por Sylte, Matthew J., Sivasankaran, Sathesh K., Trachsel, Julian, Sato, Yuko, Wu, Zuowei, Johnson, Timothy A., Chandra, Lawrance C., Zhang, Qijing, Looft, Torey“…Poults were inoculated by oral gavage with CcK (5 × 10(7) colony forming units) or sterile-media (mock-colonized), and euthanized at 1 and 3 dpi. …”
Publicado 2021
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38330por Liu, Wenjie, Xie, Gang, Yuan, Guixin, Xie, Dantao, Lian, Zhen, Lin, Zihong, Ye, Jiajie, Zhou, Wenyun, Zhou, Weijun, Li, Henghui, Wang, Xinjia, Feng, Haotian, Liu, Ying, Yao, Guanfeng“…After the culture of primary murine bone marrow-derived macrophages/monocytes were induced by the use of macrophage colony-stimulating factor (M-CSF) and the receptor activator of NF-κB ligand (RANKL) and then treated with GPF. …”
Publicado 2021
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38331por Oro, Violeta, Stanisavljevic, Rade, Nikolic, Bogdan, Tabakovic, Marijenka, Secanski, Mile, Tosi, Solveig“…Following fungal isolation and colony growing, the fungal DNA was extracted, the ITS region was amplified, and PCR products were sequenced. …”
Publicado 2021
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38332por Lu, Sheng-Shan, Takahashi, Junichi, Yeh, Wen-Chi, Lu, Ming-Lun, Huang, Jing-Yi, Lin, Yi-Jing, Sung, I-Hsin“…ABSTRACT: The invasive alien species (IAS) Vespa bicolor is the first reported hornet that has established in Taiwan and is concerning as they prey on honeybee Apis mellifera, which leads to colony losses and public concerns. Thus, the aim of this study was to assess the current status of V. bicolor abundance, dispersal, and impact and to trace the origins of Taiwan’s V. bicolor population. …”
Publicado 2021
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38333por Fayaz, H., Afzal, Asif, Samee, A. D. Mohammed, Soudagar, Manzoore Elahi M., Akram, Naveed, Mujtaba, M. A., Jilte, R. D., Islam, Md. Tariqul, Ağbulut, Ümit, Saleel, C. Ahamed“…Critical review on use of various optimization algorithms (like ant colony, genetic, particle swarm, response surface, NSGA-II, etc.) for design parameter optimization are presented and categorized for different BTMS to boost their objectives. …”
Publicado 2021
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38334“…Finally, ΔTAD-GRHL2 mutants reduced growth and colony formation relative to wild-type controls. Together, this work will provide an understanding of how transcriptionally active ER and GRHL2 selectively cooperate to regulate transcription, growth, and differentiation in ER-positive breast cancer.…”
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38335por Yayo, Johannes, Kuil, Teun, Olson, Daniel G., Lynd, Lee R., Holwerda, Evert K., van Maris, Antonius J. A.“…Growth on both glucose and fructose was achieved with biomass yields of 0.09 ± 0.00 and 0.18 ± 0.00 g(biomass) g(substrate)(−1), respectively, compared to 0.15 ± 0.01 g(biomass) g(substrate)(−1) for wild type on cellobiose. Single-colony isolates had no or short lag times on the monosaccharides, while wild type showed 42 ± 4 h on glucose and >80 h on fructose. …”
Publicado 2021
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38336por Vandenplas, Yannick, Simoens, Steven, Van Wilder, Philippe, Vulto, Arnold G., Huys, Isabelle“…TNF-inhibitors, insulins, granulocyte colony-stimulating factors, epoetins, rituximab, trastuzumab). …”
Publicado 2021
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38337por Tian, Xin, Wu, Lina, Jiang, Min, Zhang, Zhenyong, Wu, Rong, Miao, Jianing, Liu, Caigang, Gao, Song“…The MDA-MB 231 cell line was used for GLYAT gene knockdown experiments while the MCF7 cell line for overexpression experiments. Colony formation experiments, soft agar experiments, and transwell assays were utilized for further inspection of cell proliferation and migration capabilities. …”
Publicado 2021
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38338por Lee, Seong Shin, Paradhipta, Dimas Hand Vidya, Lee, Hyuk Jun, Joo, Young Ho, Noh, Hyeon Tak, Choi, Jeong Seok, Ji, Keum Bae, Kim, Sam Churl“…All WCR forages were applied distilled water (CON) or mixed inoculants with 1:1 ratio at 1×10(5) colony forming unit/g (INO), and ensiled into 20 L mini silo (5 kg) in quadruplicates for 108 d. …”
Publicado 2021
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38339por Møller, Cleide Oliveira de Almeida, Freire, Luisa, Rosim, Roice Eliana, Margalho, Larissa Pereira, Balthazar, Celso Fasura, Franco, Larissa Tuanny, Sant’Ana, Anderson de Souza, Corassin, Carlos Humberto, Rattray, Fergal Patrick, de Oliveira, Carlos Augusto Fernandes“…The inhibitory effect of these LAB strains was confirmed by the reduction of fungus colony size, suggesting dominance of LAB by competition (a Lotka-Voltera effect). …”
Publicado 2021
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38340por Vornhagen, Jay, Bassis, Christine M., Ramakrishnan, Srividya, Hein, Robert, Mason, Sophia, Bergman, Yehudit, Sunshine, Nicole, Fan, Yunfan, Holmes, Caitlyn L., Timp, Winston, Schatz, Michael C., Young, Vincent B., Simner, Patricia J., Bachman, Michael A.“…Next, we employed multiple mouse models of infection and colonization to show that 1) the ter operon is dispensable during bacteremia, 2) the ter operon enhances fitness in the gut, 3) this phenotype is dependent on the colony of origin of mice, and 4) antibiotic disruption of the gut microbiota eliminates the requirement for ter. …”
Publicado 2021
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