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38361por Brown, J. Morgan, van Loon, E. Emiel, Bouten, Willem, Camphuysen, Kees C. J., Lens, Luc, Müller, Wendt, Thaxter, Chris B., Shamoun‐Baranes, Judy“…Using high‐resolution multi‐year GPS tracking data from 82 individuals from eight colonies in Western Europe, we quantified inter‐ and intra‐individual variation in non‐breeding distributions, winter site fidelity, migration routes and timing of migration, with the objectives of determining how much variation lesser black‐backed gulls have in their migratory behaviour and examining whether variation changes with migration distance. 4. …”
Publicado 2021
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38362“…Methods. Cell proliferation, colony formation, and EdU assay were performed to identify the cytotoxicity of curcumin and probucol on chondrocytes. …”
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38363por Guan, Yunzhi, Sun, Chi, Zou, Fei, Wang, Hongli, Lu, Feizhou, Song, Jian, Liu, Siyang, Xia, Xinlei, Jiang, Jianyuan, Ma, Xiaosheng“…Next, Alizarin red staining, Alcian blue staining and TEM were performed to evaluate the effects of CESCs into osteoblasts and chondroblasts, respectively, CCK8 for the cell proliferation of osteoblasts and chondroblasts after induction for different times; cell cycle of osteoblasts or chondroblasts was measured by flow cytometry after induction, and WB for the measurement of specific biomarkers of OC and RUNX in osteoblasts and aggrecan, collagen II in chondroblasts. Finally, colony formation was applied to measure the cell proliferation of CESCs transfected with ov‐CHST3 or sh‐CHST3 when cocultured with bone marrow cells, WB for the protein expression of CHST3, CSPG4 and ELAVL1 in CSECs, transwell assay for the migration of CESCs to bone marrow cells, TEM image for the cellular characteristics of bone marrow cells, and WB for the protein profile of VCAN, VASP, NCAN and OFD1 in bone marrow cells. …”
Publicado 2021
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38364por Zhang, Xilin, He, Ying, Jiang, Yan, Bao, Ying, Chen, Qiuqiang, Xie, Dong, Yu, Huanming, Wang, Xiang“…The effects of TMEM229A on cell proliferation, migration and invasion were detected using Cell Counting Kit-8, colony formation, soft agar, real-time cellular analysis and Transwell assays. …”
Publicado 2021
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38365“…The mRNA expression of TPD52, miR-218-5p or NEAT1 were tested by Rt-qPCR and the protein expression of TPD52 was tested by western blot. Colony formation and EdU assays were carried out to evaluate cell proliferation. …”
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38366por Aeby, Greta Smith, Shore, Amanda, Jensen, Thor, Ziegler, Maren, Work, Thierry, Voolstra, Christian R.“…Coral reefs of the central Red Sea had a widespread but a surprisingly low prevalence of disease (<0.5%), based on the examination of >75,750 colonies. Twenty diseases were recorded affecting 16 coral taxa and included black band disease, white syndromes, endolithic hypermycosis, skeletal eroding band, growth anomalies and focal bleached patches. …”
Publicado 2021
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38367por Hebert, Kassidy A., Jaramillo, Sergio, Yu, Wangjie, Wang, Min, Veeramachaneni, Ratna, Sandulache, Vlad C., Sikora, Andrew G., Bonnen, Mark D., Annapragada, Ananth V., Corry, David, Kheradmand, Farrah, Pandita, Raj K., Ludwig, Michelle S., Pandita, Tej K., Huang, Shixia, Coarfa, Cristian, Grimm, Sandra L., Perera, Dimuthu, Miles, George, Ghebre, Yohannes T.“…For the in vitro studies, we used cancer cell colony formation (clonogenicity) assay to compare cancer cell growth in the absence or presence of esomeprazole. …”
Publicado 2021
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38368“…Then, PC9/GR cells were transfected with miR‐133a‐3p mimics and PC9 cells were transfected with miR‐133a‐3p inhibitors to increase or decrease the expression of miR‐133a‐3p. CCK‐8 assay, colony formation assay, and caspase‐3 activity assay were employed to detect cell resistance to gefitinib. …”
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38369“…Cell Counting Kit-8 (CCK-8), colony formation, and 5-Ethynyl-2ʹ-deoxyuridine (Edu) assays were utilized to determine cell proliferation. …”
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38370por Abbott-Johnson, Kaitlin, Pierce, Kursten V., Roof, Steve, del Rio, Carlos L., Hamlin, Robert“…Animals: Seven purpose-bred Beagles were studied from a research colony with tachycardia induced DCM phenotype. Methods: The effects of pimobendan were studied under a placebo-controlled single-blinded cross-over design. …”
Publicado 2021
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38371“…Their expression levels were examined by RT‐PCR. CCK8, colony formation, migration, and invasion assay were conducted in LUAD cells which had silenced SGMS1‐AS1. …”
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38372por Pan, Di, Zhang, Wen, Zhang, Nenling, Xu, Yini, Chen, Yi, Peng, Jianqing, Chen, Yan, Zhang, Yanyan, Shen, Xiangchun“…In this study, we investigated whether synergistic effects exist with the combination treatment with OMT and DOX using human colorectal cancer cell (CRC) lines and the potential mechanisms involved in in vitro and in vivo activities. The MTT and colony formation assay results showed that compared to either OMT or DOX monotherapy, the combination of OMT + DOX markedly inhibited the growth of HT-29 and SW620 cells. …”
Publicado 2021
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38373por Zuo, Xin, Li, Weiling, Yan, Xiaofang, Ma, Tieliang, Ren, Yan, Hua, Meijuan, Yang, Huiyun, Wu, Haifeng, Zhu, Hongdi“…Cell Counting Kit-8, colony formation and flow cytometry assays were used to assess cell proliferation and apoptosis. …”
Publicado 2021
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38374por Wei, Yuang, Chen, Xinglin, Ren, Xiaohan, Wang, Bao, Zhang, Qian, Bu, Hengtao, Qian, Jian, Shao, Pengfei“…Clinical correlation analysis and Gene Set Variation Analysis (GSVA) on MX2 showed that the upregulation of MX2 was significantly related to the malignant phenotype of ccRCC, and it was involved in several pathways and biological processes associated with anticancer drug resistance. qRT-PCR and Western blotting revealed that MX2 was distinctly upregulated in sunitinib-resistant RCC cell lines. Colony formation assay and Cell Counting Kit-8 (CCK8) assay showed that MX2 strongly promoted resistant capability to sunitinib of ccRCC cells. …”
Publicado 2021
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38375por Sansupa, Chakriya, Fareed Mohamed Wahdan, Sara, Disayathanoowat, Terd, Purahong, Witoon“…Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. …”
Publicado 2021
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38376“…Further, the effect of concurrent and sequential administration of Fulvestrant–Everolimus combination on colony formation was determined. The studies showed that in cells expressing wild type p53, as well as in cells devoid of p53, ERα represses AMPK, whereas in cells harboring mutant p53, repression of AMPK is sustained even in the absence of ERα. …”
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38377“…In eusocial insects, colony function is maintained by a division of labor between reproductive and non-reproductive castes, and chemosensation is essential for regulating their specialized social activities. …”
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38378por Chu, Yi Cheng, Tsai, Tung-Yao, Yadav, Vijesh Kumar, Deng, Li, Huang, Chun-Chih, Tzeng, Yew-Min, Yeh, Chi-Tai, Chen, Ming-Yao“…Sulforhodamine B assays, together with the clonogenicity and invasion assay, revealed that KRAS-mutant SW1463 cells were resistant to cetuximab; however, 4-AAQB treatment effectively resensitized CRC cells to cetuximab through the reduction of colony formation, invasion, and tumorsphere generation and of oncogenic KRAS signaling cascade of CRC cells. …”
Publicado 2021
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38379por Wang, Li Ming, Jung, Sungmi, Serban, Monica, Chatterjee, Avishek, Lee, Sangkyu, Jeyaseelan, Krishinima, El Naqa, Issam, Seuntjens, Jan, Ybarra, Norma“…METHODS: 25 rats were randomized into 5 groups: non-irradiated control (CG), irradiated control (CR), intraperitoneally administered granulocyte-macrophage colony stimulating factor or GM-CSF (Drug), intravascularly administered MSC (IV), and intratracheally administered MSC (IT). …”
Publicado 2021
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38380por Rocha, Leonardo Silva, Silva, Beatriz Pereira da, Correia, Thiago M.L., Silva, Railmara Pereira da, Meireles, Diogo de Abreu, Pereira, Rafael, Netto, Luis Eduardo Soares, Meotti, Flavia Carla, Queiroz, Raphael Ferreira“…A significantly lower number of colonies was detected in the lung and spleen of ΔahpC1-infected mice. …”
Publicado 2021
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