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38381por Faruque, Abu S. G., Khan, Azharul Islam, Islam, S. M. Rafiqul, Nahar, Baitun, Hossain, M. Nasif, Widiati, Yulia, Hasan, A. S. M. Mainul, Prajapati, Mukeshkumar, Kim, Minjoon, Vandenent, Maya, Ahmed, Tahmeed“…The provisionally diagnosed specimens of cholera cases were inoculated into Cary-Blair Transport Medium; then sent to the laboratory of icddr,b in Dhaka to isolate the colony as well as perform antibiotic susceptibility tests. …”
Publicado 2021
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38382por Guo, Jianfeng, Zhu, Yapei, Yu, Lili, Li, Yuan, Guo, Jing, Cai, Jing, Liu, Lin, Wang, Zehua“…METHODS: We conducted wound healing, transwell migration, EdU cell proliferation, colony formation and apoptosis detection assays to observe the effects of aspirin on the migration, proliferation and apoptosis of EOC cells (A2870, Caov-3, and SK-OV-3). …”
Publicado 2021
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38383por Ebner-Peking, Patricia, Krisch, Linda, Wolf, Martin, Hochmann, Sarah, Hoog, Anna, Vári, Balázs, Muigg, Katharina, Poupardin, Rodolphe, Scharler, Cornelia, Schmidhuber, Sabine, Russe, Elisabeth, Stachelscheid, Harald, Schneeberger, Achim, Schallmoser, Katharina, Strunk, Dirk“…Methods: Primary endothelial colony forming cells (ECFCs), skin fibroblasts (FBs) and keratinocytes (KCs) were isolated from human tissues and polyclonally propagated under 2D xeno-free conditions. …”
Publicado 2021
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38384por de Santa-Inez, Daniel Casartelli, Fuziwara, Cesar Seigi, Saito, Kelly Cristina, Kimura, Edna Teruko“…As a result, we observed that the ATC cell line KTC2-Cl1 showed a 60% decrease in the expression of miR-146b-5p compared to the control, also showing reduced cell viability, migration, colony formation, and blockage of tumor development in immunocompromised mice. …”
Publicado 2021
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38385“…METHODS: Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), colony formation, and 5-ethynyl-2ʹ-deoxyuridine (EdU) assays. …”
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38386“…This study aimed to explore whether the function of lidocaine was related to the oncogenic circ_DYNC1H1 in HCC. Colony formation assay and 3-(4,5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) assay were used for proliferation detection. …”
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38387“…COL6A1 was overexpressed or knocked down, and the proliferation and invasion of NSCLC cells was assessed using Cell Counting Kit-8, colony formation and Transwell invasion assays. Additionally, the osteogenic capacity of HOB and hES-MP 002.5 cells was assessed using RT-qPCR, western blotting, Alizarin Red and alkaline phosphatase staining. …”
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38388por Olsen, Steven C., Boggiatto, Paola M., Nol, Pauline, McCollum, Matthew P., Rhyan, Jack C.“…Bison (Bison bison) heifer calves (n = 32) were randomly assigned to control or vaccination with 10(10) colony-forming units of Brucella abortus strain RB51 (RB51) vaccine by single or boostered parenteral delivery, or by surgical implantation of a dry dart formulation (n = 8/trt). …”
Publicado 2021
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38389por Huang, Yingying, Du, Xiaoxiao, Chen, Xiangliu, Chen, Chuanzhi, Wang, Haiyong, Yang, Yan, Teng, Lisong“…Cell Counting Kit-8 assay (CCK-8) and colony formation assay were used to investigate cell proliferation ability. …”
Publicado 2021
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38390por Chauhan, Kanika, Aly, Sharif S., Lehenbauer, Terry W., Tonooka, Karen H., Glenn, Kathy, Rossitto, Paul, Marco, Maria L.“…In milk, the lower limit of detection of M. bovis, M. californicum, and A. laidlawii with the multiplex assay was between 120 to 250 colony forming units (CFU) per mL. The assay was also able to simultaneously detect both M. bovis and A. laidlawii in milk when present in moderate (10(3) to 10(4) CFU/mL) to high (10(6) to 10(7) CFU/mL) quantities. …”
Publicado 2021
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38391por Sarro, Yeya dit Sadio, Butzler, Matthew A, Sanogo, Fanta, Kodio, Ousmane, Tolofoudie, Mohamed, Goumane, Mariam S, Baya, Bocar, Diabate, Seydou, Diallo, Ibrahim Boubacar, Daniogo, Djakaridja, Dembele, Bindongo PP, Camara, Issiaka, Kumar, Alisha, Dembele, Etienne, Kone, Bourahima, Achenbach, Chad J, Theron, Grant, Ouattara, Khadidia, Toloba, Yacouba, Diarra, Bassirou, Doumbia, Seydou, Taiwo, Babafemi, Holl, Jane L, Murphy, Robert L, Diallo, Souleymane, McFall, Sally M, Maiga, Mamoudou“…FINDINGS: In the development stage, the new assay showed a high analytic performance with 100% detections of MTBC and MAC at only 5 colony forming units (CFUs). Overall, without the treatment failure cases, the Multiplex assay and the Xpert showed a sensitivity, specificity, PPV and NPV of 83·3% [66·4-92·6], 96·6% [88·6-99·0], 92·5% [82·3-96·5] and 92·2% [82·7-96·5] and the Xpert had values of 96·7% [83·3-99·4], 80·0% [68·2-88·1], 70·7 [55·5-82·3] and 97·9% [89·3-99·6], respectively. …”
Publicado 2021
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38392“…In the present study, circ-PVT1 was knocked down in A549 cells and the cell viability, proliferation, migration and invasion were measured via MTT, colony formation, wound healing and Transwell assays, respectively. …”
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38393“…Kombucha is produced by fermenting tea using a “symbiotic colony of bacteria and yeast” (SCOBY). Kombucha is marketed as a non-alcoholic beverage, however due to the production process employed, there is a high possibility that the Kombucha products will contain low levels of ethanol. …”
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38394por Geng, Hui, Liu, Guangli, Hu, Jiandong, Li, Jianchao, Wang, Dong, Zou, Shenshan, Xu, Xintao“…Following HOXB13 overexpression (Ov-HOXB13), HGC-27 cell proliferation was evaluated by colony formation and Cell Counting Kit-8 assays. Wound healing and Matrigel assays were used to determine the migratory and invasive abilities, respectively. …”
Publicado 2021
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38395“…Functional experiments, including Cell Counting Kit-8, colony formation, cell cycle assays and western blotting, were conducted to examine the effects of FoxD2-AS1 and miR-4306 on the malignant behaviors of CRC cells. …”
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38396por Wang, Baikui, Gong, Li, Zhou, Yuanhao, Tang, Li, Zeng, Zihan, Wang, Qi, Zou, Peng, Yu, Dongyou, Li, Weifen“…Broilers were fed with either a basal diet or basal diets supplemented with 1 × 10(8) colony-forming units (CFU)/kg P. polymyxa 10 (BSC10) or L. plantarum 16 (Lac16) for 42 d. …”
Publicado 2021
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38397por Brajon, Sophie, Morello, Gabriela Munhoz, Capas-Peneda, Sara, Hultgren, Jan, Gilbert, Colin, Olsson, Anna“…Beyond considerations of animal welfare and ethics, and a conflict with the 3Rs principle, high perinatal mortality means that larger colonies of breeding animals are needed to supply animals in sufficient numbers for research. …”
Publicado 2021
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38398por Fu, Xue, Feng, Lan, Kong, Linghan, Li, Chun, Zhao, Xiaodong, Li, Huade, Cui, Pengfei, Yan, Wenjun, Zhai, Yaru, Zhang, Lan, Li, Hao, Wang, Hongning, Yang, Xin“…Animal experiments showed that the lethal dose (LD(50)) was 4.57 × 10(7) CFU (colony-forming units); the shedding time of S. Derby in mice was 24 days; the bacterial loads in spleen were higher than those in other organs (ileum, liver, and cecum). …”
Publicado 2021
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38399“…Results: Single biofilms of S. aureus, P. aeruginosa, and K. variicola grew to approximately 1 × 10(6) colony forming units (CFU)/mL and C. albicans to 1 × 10(5) CFU/mL. …”
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38400por Wang, Shao-Chuan, Chang, Ya-Chuan, Wu, Min-You, Yu, Chia-Ying, Chen, Sung-Lang, Sung, Wen-Wei“…Azacitidine significantly inhibited DNMT expression in UBUC cell lines and reduced cell viability and clonogenic activity, as determined by MTT and colony formation assays, while also inducing significant cytotoxic effects in the form of increased sub-G1 and Annexin V-PI populations (all p < 0.05). …”
Publicado 2021
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