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38461“…Cell biology assays were used to investigate biologic consequences of MIR17HG in regulating cell proliferation, colony formation and invasion, as well as the roles in regulating epithelial-mesenchymal transition (EMT). …”
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38462“…METHODS: The 5-FU resistant HCT116 cell line (HCT116/5-FU) was established by repeated exposure to gradually increasing concentrations of 5-FU; Cell viability was measured by cell counting kit-8 (CCK-8); apoptosis rate of HCT116 cells was detected using Annexin V-fluorescein isothiocyanate (FITC) assay kit; cell proliferation and invasion were detected using colony formation assays, wound healing assay and transwell invasion assays; activity of transplanted tumor in vivo in specific pathogen free (SPF) BALB/c nude mice (6 weeks old, male) was monitored by bioluminescence imaging, immunohistochemistry and western blot analysis. …”
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38463por Wang, Hong-Qun, Li, Ying, Ding, Shan-Shan, Li, Ying-Xue, Wang, Ai-Chun, Shi, Huai-Yin“…A cell viability test showed that the differences between the groups increased on days 4 and 5 (P<0.05). The number of colony-forming cells had also decreased significantly after 10 days (P<0.05) in the USP22-siRNA1 group. …”
Publicado 2020
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38464“…Additionally, cell proliferation and apoptosis assay were performed via CCK8, colony formation and Annexin V-FITC apoptosis assay. …”
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38465“…Cell proliferative potential was estimated by colony formation and Cell Counting Kit-8 (CCK-8) assays. …”
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38466por Yuan, Jing, Jiang, Xiaoyan, Lan, Hua, Zhang, Xiaoyu, Ding, Tianyi, Yang, Fan, Zeng, Da, Yong, Jiahui, Niu, Beibei, Xiao, Songshu“…Then, we performed proliferation, colony formation, migration, and invasion assays to investigate the impact of MAL2 in OC cell lines in vivo and in vitro. …”
Publicado 2022
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38467“…The progression was investigated by cell counting kit-8 (CCK-8), colony formation, transwell and xenograft analysis. …”
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38468“…RNase R treatments, RNA stability assay and nucleoplasmic separation assay were conducted to identify the characteristics of circVRK1. We adopted CCK-8, colony formation, wound-healing, and transwell assays to assess the biological effects of circVRK1 on the proliferation, migration, and invasiveness of osteosarcoma cells in vitro. …”
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38469“…WT cells produced filamentous colonies in all concentrations. Together, the data indicate that Dfi1, Czf1, Sef1, and environmental iron regulate C. albicans contact-dependent filamentation. …”
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38470“…Glutamine uptake, cell proliferation and cell apoptosis were investigated by glutamine uptake, cell counting kit-8, cell colony formation, 5-Ethynyl-29-deoxyuridine, flow cytometry analysis or DNA content quantitation assay. …”
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38471“…Stories of ancestral teachings about health and prevention shared within the Danza circle create spaces wherein participants navigate complex conversations that resist oppressive colonial narratives, reconnect with and strengthen their Indigenous identities, and strive toward ancestral visions of health and well-being. …”
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38472por Park, Jimin, Kim, Seung Ki, Hallis, Steffanus Pranoto, Choi, Bo-Hyun, Kwak, Mi-Kyoung“…CSC-like properties, including sphere formation, motility, migration, colony formation, and anticancer resistance, were enhanced in the CD133(high) population compared to CD133(low) HCT116 cells. …”
Publicado 2022
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38473“…Cellular processes, including proliferation, migration and invasion were assessed using colony formation, wound healing and Transwell assays, respectively. mRNA and protein expression levels were determined using reverse transcription-quantitative PCR and western blot analysis, respectively. …”
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38474por Liu, Wei, Deng, Lisha, Xu, Anchun, Xiong, Xingcheng, Tao, Jing, Chang, Jian, Xu, Yiling, Zhou, Zhilin“…Next, Cell or animal models were constructed, CCK-8, cell colony, EdU, flow cytometry assay, transwell migration, and Western blot assays were applied. …”
Publicado 2022
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38475por Bouby, Laurent, Bonhomme, Vincent, Cabanis, Manon, Durand, Frédérique, Figueiral, Isabel, Flottes, Laurie, Marinval, Philippe, Martin, Lucie, Paradis, Laure, Pinaud, Rachël, Ros, Jérôme, Rovira, Núria, Tillier, Margaux“…Domesticated fruits together with cultivation techniques apparently reached the western Mediterranean via colonial activities during the 1st millennium BCE – early 1st millennium CE. …”
Publicado 2022
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38476por Galetzka, Danuta, Böck, Julia, Wagner, Lukas, Dittrich, Marcus, Sinizyn, Olesja, Ludwig, Marco, Rossmann, Heidi, Spix, Claudia, Radsak, Markus, Scholz-Kreisel, Peter, Mirsch, Johanna, Linke, Matthias, Brenner, Walburgis, Marron, Manuela, Poplawski, Alicia, Haaf, Thomas, Schmidberger, Heinz, Prawitt, Dirk“…Molecular karyotyping of AML samples during therapy with hypermethylated RAD9A showed an evolving duplication of 1.8 kb on Chr16p13.3 including the PKD1 gene. Radiation, colony formation assays, cell proliferation, PCR and molecular karyotyping SNP-array experiments using generated FaDu subclones suggest that hypermethylation of RAD9A intron 2 is associated with genomic imbalances in regions with tumor-relevant genes and survival of the cells. …”
Publicado 2022
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38477“…The MTT assay, the plate cloning assay, the wound healing assay, and the Transwell assay were used to detect cell viability, colony formation, cell migration, and cell invasion, respectively. …”
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38478por Muzeniek, Therese, Perera, Thejanee, Siriwardana, Sahan, Bas, Dilara, Kaplan, Fatimanur, Öruc, Mizgin, Becker-Ziaja, Beate, Perera, Inoka, Weerasena, Jagathpriya, Handunnetti, Shiroma, Schwarz, Franziska, Premawansa, Gayani, Premawansa, Sunil, Yapa, Wipula, Nitsche, Andreas, Kohl, Claudia“…We present the genome sequence of a novel α-CoV strain detected in rectal swab samples of Miniopterus fuliginosus bats from a colony in the Wavul Galge cave (Koslanda, Sri Lanka). …”
Publicado 2022
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38479por Richomme, Céline, Lesellier, Sandrine, Salguero, Francisco Javier, Barrat, Jacques Laurent, Boucher, Jean-Marc, Reyes-Reyes, Jennifer Danaidae, Hénault, Sylvie, De Cruz, Krystel, Tambosco, Jennifer, Michelet, Lorraine, Boutet, Justine, Elahi, Rubyat, Lyashchenko, Konstantin P., O’Halloran, Conor, Balseiro, Ana, Boschiroli, Maria Laura“…In order to understand the pathogenesis of M. bovis infection in foxes and the associated risk of transmission, 12 captive foxes (6 females and 6 males) were inoculated orally with 2 × 10(7) colony-forming units of a French field isolate of M. bovis. …”
Publicado 2022
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38480por Li, Jie, Wong, Ronald Man Yeung, Chung, Yik Lok, Leung, Sharon Shui Yee, Chow, Simon Kwoon-Ho, Ip, Margaret, Cheung, Wing-Hoi“…Staphylococcus aureus at 4 × 10(4) colony-forming units (CFU)/ml was inoculated. Rats were euthanized at four and eight weeks post-surgery. …”
Publicado 2022
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