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  1. 38621
    “…Our results showed that the plasmid pBV220-E-aa-SNA containing E. coli was efficiently cleaved at 42°C with 94.8% positive ratio as assessed by colony counts. Transmission electron microscopy (TEM) confirmed bacteria retained intact surface structure while devoid of cytoplasmic component. …”
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    Comparative study of subcutaneous, intramuscular, and oral administration of bovine pathogenic Escherichia coli bacterial ghost vaccine in mice
  2. 38622
    “…Granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon gamma (IFN-gamma), interleukin (IL)-1 beta, IL-2, Il-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-27, and tumor necrosis factor (TNF)-alpha were measured in patients’ serum with a Th1/Th2/Th9/Th17/Th22/Treg Cytokine 18-Plex Human ProcartaPlex Panel, based on Luminex xMAP technology. …”
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    Multiple Cytokine Analysis of Th1/Th2/Th9/Th17/Th22/Treg Cytokine Pathway for Individual Immune Profile Assessment in Patients with Psoriasis
  3. 38623
    “…Co-administration of ADCs with colony-stimulating factors, proton pump inhibitors, H2-receptor antagonists, or CYP3A4/5 inhibitors showed to synergistically increase the risk of sepsis-related toxicities. …”
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    Do antibody–drug conjugates increase the risk of sepsis in cancer patients? A pharmacovigilance study
  4. 38624
    “…To generate competent antigen presenting cells from PBMCs, we promoted differentiation of PBMCs into dendritic cells with granulocyte macrophage colony stimulating factor and IL-4. RESULTS: We observed robust dendritic cell differentiation from human PBMCs treated with 50 ng/mL GM-CSF and 20 ng/mL IL-4 in as little as 3 days. …”
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    Preservation of antigen-specific responses in cryopreserved CD4(+) and CD8(+) T cells expanded with IL-2 and IL-7
  5. 38625
    “…The three cultivars reached colony formation but no further development was observed in this culture method. …”
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    Plant regeneration from leaf mesophyll derived protoplasts of cassava (Manihot esculenta Crantz)
  6. 38626
    “…The assay conditions were optimized as follows: 10-μl loopful of bacterial colonies was suspended in 150 μl 0.01 M Na-PBS buffer, pH 7.4, a 10 μg ertapenem susceptibility-testing disk was immersed in the suspension and incubated 3 h at 35°C, after which supernatant was obtained by centrifugation and applied on a target plate with alpha-cyano-4-hydroxycinnamic acid matrix. …”
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    Detection of carbapenemase-producing Enterobacterales by means of matrix-assisted laser desorption ionization time-of-flight mass spectrometry with ertapenem susceptibility-testing disks as source of carbapenem substrate
  7. 38627
    “…RESULTS: A 17 year-old girl who had long standing oral ulcers and developed IBD, requiring systemic steroid and regular granulocyte colony-stimulating factor (GCSF) therapy and an 8 year-old boy who had steady non healing oral lesions were treated with empagliflozin during 18–24 months. …”
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    Favorable outcome of empagliflozin treatment in two pediatric glycogen storage disease type 1b patients
  8. 38628
    por Cai, Jian, Zhao, Juan, Gao, Ping, Xia, Yuguo
    Publicado 2022
    “…Cell proliferation, migration, and invasion were assessed by colony formation, 5-Ethynyl-2’-deoxyuridine staining, cell scratch assays, and Transwell invasion assays, respectively. …”
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    Patchouli alcohol inhibits GPBAR1-mediated cell proliferation, apoptosis, migration, and invasion in prostate cancer
  9. 38629
    “…Expression of these five genes in LUAD samples and adjacent normal tissues were evaluated by qRT-PCR. Colony formation and wound healing assays were performed to assess the significance of CNIH1 and AP3S1 in LUAD cells. …”
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    Vesicle-mediated transport-related genes are prognostic predictors and are associated with tumor immunity in lung adenocarcinoma
  10. 38630
    “…The functional importance of miR-27b-3p, PVT1 and BLM within PCa cells was assessed through the in vitro utilization of Cell Counting Kit-8, Transwell, wound healing and colony formation assays, and the in vivo use of a mouse xenograft model. …”
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    N(6)-methyladenosine-induced long non-coding RNA PVT1 regulates the miR-27b-3p/BLM axis to promote prostate cancer progression
  11. 38631
    “…OMV-mediated gene transfer in liquid cultures and the acquisition of the carbapenem gene and virulence gene was confirmed using colony-PCR. Antimicrobial susceptibility testing, mCIM and eCIM were conducted for the resistance of transformant. …”
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    Horizontal gene transfer via OMVs co-carrying virulence and antimicrobial-resistant genes is a novel way for the dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae
  12. 38632
    “…Antibacterial activity was described as bactericidal or bacteriostatic, defined as a ≥ 3-log(10) colony-forming units (CFU)/mL or < 3-log(10) CFU/mL decrease from initial inoculum, respectively. …”
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    1522. Penicillin plus ceftaroline against Enterococcus faecalis blood isolates lacking ampicillin plus ceftriaxone activity
  13. 38633
    “…Dual-luciferase reporter assays were applied to verify the targeted interaction relationship. CCK-8, colony formation, flow cytometry, wound healing, and Transwell assays were performed to assess the proliferation, apoptosis, migration, and invasion of GC cells. …”
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    MiR-378a-3p acts as a tumor suppressor in gastric cancer via directly targeting RAB31 and inhibiting the Hedgehog pathway proteins GLI1/2
  14. 38634
    “…METHODS: A total of 699 bacterial isolates were collected from wound swabs and each isolate was identified using gram staining, biochemical assays, antibiotic susceptibility tests with the disk diffusion method, and colony morphology. Samples were taken from January 2018 to December 2019. …”
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    Isolation of multidrug resistance bacteria from the patients with wound infection and their antibiotics susceptibility patterns: A cross-sectional study
  15. 38635
    por Liu, Jing, Song, Jinlan, Li, Chao
    Publicado 2022
    “…The functions of MYBPC1 in the laryngeal carcinoma cell lines were examined using colony formation assay, cell proliferation and invasion assays, and via measuring the extent of apoptosis. …”
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    MYBPC1 is a key regulator for laryngeal carcinoma formation
  16. 38636
    “…Cell proliferation was analyzed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide and colony formation assays. Flow cytometry was conducted to analyze cell apoptosis rate. …”
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    LOC554202 contributes to chordoma progression by sponging miR-377-3p and up-regulating SMAD3
  17. 38637
    por Nishioka, Tomoki, Tamaki, Hideyuki
    Publicado 2022
    “…Intriguingly, the majority of these recalcitrant bacteria formed colonies faster on PS or GG medium than on PT medium, which may have contributed to their successful isolation. …”
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    Improved Cultivation and Isolation of Diverse Endophytic Bacteria Inhabiting Dendrobium Roots by Using Simply Modified Agar Media
  18. 38638
    “…When competent BW3KD cells were prepared using this developed method, the transformation efficiency reached up to (7.21 ± 1.85) × 10(9) CFU/μg DNA, which exceeded the levels of commercial chemically competent cells and homemade electrocompetent cells. BW3KD cells formed colonies within 7 h on lysogeny broth agar plates, quicker than the well-known fast-growing E. coli cloning strain Mach1. …”
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    An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
  19. 38639
    “…Acquired drug resistance to macrolides and aminoglycosides was analyzed through sequencing of the 23S rRNA gene rrl and the 16S rRNA gene rrs from independent drug-resistant colonies of both strains. When the acquisition of clarithromycin resistance was examined, a different mutational profile was detected in the M. abscessus ΔnucS strain compared with the wild-type one. …”
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    Noncanonical Mismatch Repair Protein NucS Modulates the Emergence of Antibiotic Resistance in Mycobacterium abscessus
  20. 38640
    “…BruSIC yielded faster colony growth than BSM and CM and similar CFU numbers than BAB (including for B. ovis in BAB-Serum) and inhibited accompanying microorganisms in sheep and cow samples as effectively as BSM. …”
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    BruSIC: a novel selective medium for the primary isolation of Brucella in veterinary samples
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