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38801por Qiu, Wancheng, Chang, Yanhua, Liu, Jing, Yang, Xu, Yu, Yan, Li, Jiajia, Liang, Qing, Sun, Guangchun“…P-Rex1 knockdown and overexpression cell lines were established using a recombinant lentiviral transfection system. BrdU and colony formation assays were performed to determine cell viability. …”
Publicado 2020
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38802por Sadeghi, Fatemeh, Asgari, Marzieh, Matloubi, Mojdeh, Ranjbar, Maral, Karkhaneh Yousefi, Nahid, Azari, Tahereh, Zaki-Dizaji, Majid“…The genomic instability and DNA repair capacity of breast cancer patients with BRCA1/2 mutations have been analyzed in different studies using a variety of assays, including micronucleus assay, comet assay, chromosomal assay, colony-forming assay, γ -H2AX and 53BP1 biomarkers, and fluorescence in situ hybridization. …”
Publicado 2020
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38803por El-Korany, Sarah Mohamed, Helmy, Omneya Mohamed, El-Halawany, Ali Mahmoud, Ragab, Yasser El-Mohammadi, Zedan, Hamdallah Hafez“…It was identified as Aspergillus oryzae using colony morphology, microscopical characteristics, 18S rDNA sequencing, and molecular phylogeny. …”
Publicado 2020
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38804por Tusa, Ignazia, Cheloni, Giulia, Poteti, Martina, Silvano, Angela, Tubita, Alessandro, Lombardi, Zoe, Gozzini, Antonella, Caporale, Roberto, Scappini, Barbara, Dello Sbarba, Persio, Rovida, Elisabetta“…Progenitor/stem cell potential was estimated by serial colony formation ability (CFA) assay. RESULTS: Ponatinib was more effective than imatinib for the reduction of cells with ALDH activity and progenitor/stem cell potential of CML patient-derived cells and cell lines. …”
Publicado 2020
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38805por Hagan, Nellwyn, Kane, John L., Grover, Deepak, Woodworth, Lisa, Madore, Charlotte, Saleh, Jacqueline, Sancho, Jose, Liu, Jinyu, Li, Yi, Proto, Jonathan, Zelic, Matija, Mahan, Amy, Kothe, Michael, Scholte, Andrew A., Fitzgerald, Maria, Gisevius, Barbara, Haghikia, Aiden, Butovsky, Oleg, Ofengeim, Dimitry“…Multiple sclerosis (MS) is a chronic, inflammatory, demyelinating disease of the CNS, and activated microglia and macrophages play a significant role in mediating disease pathophysiology and progression. Colony-stimulating factor-1 receptor (CSF1R) and its ligand CSF1 are elevated in CNS tissue derived from MS patients. …”
Publicado 2020
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38806por Campos, Kauara Brito, Martins, Ademir Jesus, Rodovalho, Cynara de Melo, Bellinato, Diogo Fernandes, Dias, Luciana dos Santos, Macoris, Maria de Lourdes da Graça, Andrighetti, Maria Teresa Macoris, Lima, José Bento Pereira, Obara, Marcos Takashi“…To monitor natural populations, sampling was performed in 132 Brazilian cities, using egg traps. Colonies were raised in the laboratory for one or two generations (F1 or F2) and submitted to susceptibility tests, where larvae were exposed to the pyriproxyfen DD (0.03 µg/l) and adults, to the malathion DD determined in the present study (20 µg), in addition to the one established by the World Health Organization (WHO) DD (50 µg) in a bottle assay. …”
Publicado 2020
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38807“…METHODS: We applied treatment with PLX3397 (150 ppm in the chow), a potent inhibitor of the colony stimulating factor-1 receptor (CSF-1R) to target innate immune cells in CLN1 mice. …”
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38808“…Cell proliferation was determined by cell counting kit-8 (CCK-8) assay and cell colony formation assay. Cell apoptosis and metastasis were detected by flow cytometry analysis and transwell assay, respectively. …”
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38809“…METHODS: Flow cytometry was used to analyze cell cycle progression and apoptosis. Colony formation assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay were used to analyze cell proliferation. …”
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38810por Nelson, Vinod kumar, Sahoo, Nalini Kanta, Sahu, Madhusmita, Sudhan, Hari hara, Pullaiah, Chitikela P., Muralikrishna, Kanuri Sai“…We have also studied the effects on normal human embryonic lung fibroblast cell (WI-38) using MTT (methyl thiazoldiphenyltetrazolium bromide) assay, clonogenic (colony formation) and migration assay. Finally obtained results were analyzed using ANNOVA and Dunnett’s test. …”
Publicado 2020
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38811por Xiao, Yizhi, Liu, Side, Li, Jiaying, Dai, Weiyu, Tang, Weimei, Xiang, Li, Zhang, Wenjing, Lin, Jianjiao, Wang, Jing, Wu, Xiaosheng, Liu, Guangnan, Liu, Yuyang, Chen, Yaying, Zhu, Huiqiong, Wang, Yusi, Lin, Zhizhao, Yang, Qiong, Chen, Tianming, Sun, Yong, Li, Aimin, Xiong, Jing, Wang, Jide“…METHODS: To explore the function of miR-4490 in GC, we performed colony formation, EdU incorporation, qRT-PCR, Western blotting, in situ hybridization (ISH), immunohistochemistry (IHC), flow cytometry, ChIP and dual-luciferase reporter assays. …”
Publicado 2020
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38812por Wang, Xuanchuan, Yang, Cheng, Hu, Linkun, Wei, Zheng, Tang, Qunye, Chen, Bing, Ji, Yuan, Xu, Ming, Zeng, Zhaochong, Rong, Ruiming, Zhu, Tongyu“…DHSCs were mobilized using granulocyte colony-stimulating factor (G-CSF) and harvested 1 day before transplantation. …”
Publicado 2020
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38813por Wu, Sheng, Nitschke, Katja, Worst, Thomas Stefan, Fierek, Alexander, Weis, Cleo-Aron, Eckstein, Markus, Porubsky, Stefan, Kriegmair, Maximilian, Erben, Philipp“…In vitro experiments revealed that knockdown of MIR31HG inhibits cell proliferation, colony formation, and migration in bladder cancer. …”
Publicado 2020
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38814por Gu, Meng-Li, Zhou, Xin-Xin, Ren, Meng-Ting, Shi, Ke-Da, Yu, Mo-Sang, Jiao, Wen-Rui, Wang, Ya-Mei, Zhong, Wei-Xiang, Ji, Feng“…The cell proliferation of GC cells was determined by Cell Counting Kit-8 and colony formation assays. Flow cytometry was performed following Annexin V/propidium iodide (PI) to identify apoptotic cells and PI staining to analyze the cell cycle. …”
Publicado 2020
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38815por Rojas, Laura J, Yasmin, Mohamad, Benjamino, Jacquelynn, Marshall, Steven, DeRonde, Kailynn, Krishnan, Nikhil, Perez, Federico, Colin, Andrew, Cardenas, Monica, Martinez, Octavio, Perez-Cardona, Armando, Rhoads, Daniel, Jacobs, Michael, Scott, Jacob, Adams, Mark D, Abbo, Lilian M, Abbo, Lilian M, Bonomo, Robert A“…We propose that an approach sampling the population of pathogens present in a clinical sample instead of single colonies be applied instead when dealing with XDR P. aeruginosa, especially in patients with CF. …”
Publicado 2020
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38816por Farnesi, Luana Cristina, Carvalho, Fabiano Duarte, Lacerda, Anna Paula Canuto, Moreira, Luciano Andrade, Bruno, Rafaela Vieira“…The presence of the bacterium in the colonies of Wolbachia-infected Ae. aegypti after 35 generations under the conditions evaluated indicates that they can be maintained on mouse blood. …”
Publicado 2021
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38817por Gon, Giorgia, Kabanywanyi, Abdunoor M., Blinkhoff, Petri, Cousens, Simon, Dancer, Stephanie J., Graham, Wendy J., Hokororo, Joseph, Manzi, Fatuma, Marchant, Tanya, Mkoka, Dickson, Morrison, Emma, Mswata, Sarah, Oza, Shefali, Penn-Kekana, Loveday, Sedekia, Yovitha, Virgo, Sandra, Woodd, Susannah, Aiken, Alexander M.“…We measured changes in microbiological cleanliness [Aerobic Colony Counts (ACC) and presence of Staphylococcus aureus] using dipslides, and physical cleaning action using gel dots. …”
Publicado 2021
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38818“…METHODS: Real-time PCR, RNA-pulldown, RIP, western blotting, lentivirus transfection, luciferase reporter assays, cell proliferation assays, colony formation assays, wound healing scratch assays and transwell assays were employed to examine the relationship between lncRNA CBR3-AS1 and its regulation of Wnt/β-catenin signaling in LAD cells. …”
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38819“…METHODS: The expression of HOTTIP, miR-663a and Fyn-related kinase (FRK) in the OA articular cartilage and OA chondrocyte model induced by IL-1β was determined by qRT-PCR. CCK-8, colony formation and flow cytometry were used to determine the cell proliferation and apoptosis of OA chondrocytes. …”
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38820por Chen, Li, Zuo, Yi, Pan, Ru, Ye, Zhen, Wei, Kailun, Xia, Shaohuai, Li, Wencai, Tan, Jie, Xia, Xuewei“…Cell proliferation was detected by the CCK-8 assay and colony formation analysis, cell migration and invasion checked by Transwell experiments, and cell apoptosis was determined by Annexin V/propidium iodide staining. …”
Publicado 2021
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