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38861por Parma, Beatrice, Ramesh, Vignesh, Gollavilli, Paradesi Naidu, Siddiqui, Aarif, Pinna, Luisa, Schwab, Annemarie, Marschall, Sabine, Zhang, Shuman, Pilarsky, Christian, Napoli, Francesca, Volante, Marco, Urbanczyk, Sophia, Mielenz, Dirk, Schrøder, Henrik Daa, Stemmler, Marc, Wurdak, Heiko, Ceppi, Paolo“…Real-time proliferation assays with and without cytotoxicity reagents, colony formation assays and cell cycle analyses were used to monitor growth and drug sensitivity of different NSCLC cells in vitro. …”
Publicado 2021
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38862por Tao, Bangbao, Shi, Juanhong, Shuai, Shuai, Zhou, Haiyan, Zhang, Hongxia, Li, Bin, Wang, Xiaoqiang, Li, Guohui, He, Hua, Zhong, Jun“…In in vitro assays, CYB561D2 knock-down suppressed cell growth, colony formation, migration and promoted apoptosis. …”
Publicado 2021
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38863por Mao, Changlin, Dong, Wei, Lu, Jiaju, Zhang, Zhao, Wu, Hongliang, Ghavamian, Armin, Bi, Dongbin, Gao, Pei, Liu, Zhao, Ding, Sentai“…METHODS: Western blotting was used to determine the expression of βKlotho, ELK4, and PARP in C4-2B and PC3 PCa cell lines. CCK-8 assay and colony formation assay were applied to examine the roles of βKlotho and ELK4 in the proliferation of PCa cells. …”
Publicado 2021
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38864Hsa_circ_0004674 promotes osteosarcoma doxorubicin resistance by regulating the miR-342-3p/FBN1 axis“…The proliferation and apoptosis of cells were measured using colony formation assay and flow cytometry. Western blot analysis was utilized to examine the protein levels of resistance markers, Wnt/β-catenin pathway markers and FBN1. …”
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38865por Takahashi, Hiroshi, Nakashima, Taku, Masuda, Takeshi, Namba, Masashi, Sakamoto, Shinjiro, Yamaguchi, Kakuhiro, Horimasu, Yasushi, Miyamoto, Shintaro, Iwamoto, Hiroshi, Fujitaka, Kazunori, Hamada, Hironobu, Hattori, Noboru“…RESULTS: Lung CD45(−)/ALDH(br) showed higher proliferative and colony-forming potential than cell populations with low ALDH activity. …”
Publicado 2021
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38866“…To assess the effects of LINC01503 on the resistance of OCa cells to CBP, Cell Counting Kit-8 (CCK-8), colony formation, Transwell, and flow cytometry experiments were performed to evaluate half-maximal inhibitory concentration (IC(50)), cell viability, migrative and invasive ability, as well as cell apoptosis. …”
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38867“…Cell proliferative capacity was assessed by cell counting kit-8 (CCK-8) and colony formation assays. Cell apoptosis and cell cycle distribution were monitored by flow cytometry assay. …”
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38868por Chen, Zhang-Hua, Yan, Shu-Mei, Chen, Xi-Xi, Zhang, Qi, Liu, Shang-Xin, Liu, Yang, Luo, Yi-Ling, Zhang, Chao, Xu, Miao, Zhao, Yi-Fan, Huang, Li-Yun, Liu, Bin-Liu, Xia, Tian-Liang, Xu, Da-Zhi, Liang, Yao, Chen, Yong-Ming, Wang, Wei, Yuan, Shu-Qiang, Zhang, Hui-Zhong, Yun, Jing-Ping, Zhai, Wei-Wei, Zeng, Mu-Sheng, Bai, Fan, Zhong, Qian“…Finally, the key molecular events promoting the tumor evolution were demonstrated by MTT, IC50, and colony formation assay in vitro experiments and in vivo xenograft experiments. …”
Publicado 2021
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38869por Shersher, Elena, Lahiry, Mohini, Alvarez-Trotta, Annamil, Diluvio, Giulia, Robbins, David J., Shiekhattar, Ramin, Capobianco, Anthony J.“…Gene knockdown was achieved via RNA interference and the effects of protein depletion on esophageal adenocarcinoma (EAC) proliferation were determined via a colony formation assay and murine xenografts. Western blotting was used to examine expression of INT subunits in EAC cells and evaluate apoptotic proteins upon INT subunit 11 knockdown (INTS11 KD). …”
Publicado 2021
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38870por He, Guan-Nan, Bao, Na-Ren, Wang, Shuang, Xi, Man, Zhang, Tian-Hao, Chen, Feng-Shou“…METHODS: Cell viability and proliferation were detected by cell counting kit 8 (CCK-8), colony formation, and 5-ethynyl-2′-deoxyuridine (EdU) assay. …”
Publicado 2021
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38871“…METHODS: In the current study, we employed two DNMT inhibitors (Decitabine and 5-Azacytidine), two TNBC models (MDA-MB-231 and BT-549) and whole transcriptome RNA-Seq and characterized the transcriptional alterations associated with DNMT inhibition. Colony forming unit (CFU), flow cytometry, and fluorescent microscopy were used to assess cell proliferation, cell cycle distribution, and cell death, respectively. …”
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38872por Weeraratne, Thilini Chathurika, Karunaratne, S. H. P. Parakrama, Reimer, Lisa, de Silva, W. A. Priyanka P., Wondji, Charles S.“…Furthermore, Ae. aegypti samples obtained from colonies reared at two temperatures (23 and 27 °C) were used to investigate the influence of temperature on this age-grading technique. …”
Publicado 2021
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38873por Cui, Hai-Yan, Rong, Jian-Sheng, Chen, Ju, Guo, Jie, Zhu, Jia-Qin, Ruan, Mei, Zuo, Rong-Rong, Zhang, Shuang-Shuang, Qi, Jun-Mei, Zhang, Bao-Hua“…The function and mechanism of exosomal miR-588 from M2 macrophages in the modulation of DDP resistance of gastric cancer cells was analyzed by CCK-8 assay, apoptosis analysis, colony formation assay, Western blot analysis, qPCR analysis, and luciferase reporter assay in SGC7901 and SGC7901/DDP cells, and by tumorigenicity analysis in nude mice. …”
Publicado 2021
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38874por Zhou, Ying, Zhang, Shuhong, Min, Zhonghan, Yu, Zhongwei, Zhang, Huaiwei, Jiao, Jiao“…Cell proliferation, migration, invasion and apoptosis were detected by MTT, colony formation assay, transwell, wound-healing and flow cytometry assays, respectively. …”
Publicado 2021
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38875por Skerrett-Byrne, David A., Nixon, Brett, Bromfield, Elizabeth G., Breen, James, Trigg, Natalie A., Stanger, Simone J., Bernstein, Ilana R., Anderson, Amanda L., Lord, Tessa, Aitken, R. John, Roman, Shaun D., Robertson, Sarah A., Schjenken, John E.“…Inflammation was also a key transcriptomic response to acrylamide, with the cytokine, Colony stimulating factor 2 (Csf2) identified as a top-ranked upstream driver and inflammatory mediator associated with recovery of homeostasis. …”
Publicado 2021
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38876“…Significant differences in both downstream gene expression and the biological function assays including colony formation, migration efficiency and cell apoptosis were identified in AC010198.2 knockdown cells. …”
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38877por Wang, Rui, Liu, Jian, Li, Kai, Yang, Ganghua, Chen, Sisi, Wu, Jie, Xie, Xinming, Ren, Hong, Pang, Yamei“…The self-renewal, cisplatin sensitivity and tumorigenesis of NSCLC cells were analyzed using sphere formation, CCK-8, colony formation assays and xenograft tumor models. …”
Publicado 2021
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38878por Liu, Hsien-Lin, Wang, Christine H.-T., Chiang, En-Pei Isabel, Huang, Chieh-Chen, Li, Wen-Hsiung“…Image recognition was efficiently used for high-throughput screening via colony size on solid media. In enrichment analysis of the 161 isobutanol-sensitive clones identified, more genes than expected were mapped to tryptophan biosynthesis, ubiquitination, and the pentose phosphate pathway (PPP). …”
Publicado 2021
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38879por Wangberg, Hannah, Mendoza, Jun, Gomez, Robert, Coop, Christopher, White, Andrew, Woessner, Katharine“…Symptoms were worse with exposure to her B. dubia roach colony. Skin prick testing (SPT) to B. dubia cast skin, internal organs, and feces was performed in both the subject and a human control. …”
Publicado 2021
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38880por Zamarano, Henry, Musinguzi, Benson, Kabajulizi, Immaculate, Manirakiza, Godfrey, Guti, Walker, Muhwezi, Ivan, Hussein, Ayan Ahmed, Baweera, Agnes, Kabahinda, Boaz, Itabangi, Herbert, Bazira, Joel, Kabanda, Taseera“…Specimens were inoculated in Brain heart infusion broth, transported to Fortportal Regional Referral Hospital, plated daily up to 7 days on blood, chocolate, MacConkey agar and incubated in aerobic and 5% carbondioxide. Pure colonies were identified by Gram stain, biochemical tests and antibiotic sensitivities obtained by Kirby Bauer disc diffusion method. …”
Publicado 2021
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