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39041por Domenis, Rossana, Lazzaro, Lara, Calabrese, Sarah, Mangoni, Damiano, Gallelli, Annarita, Bourkoula, Evgenia, Manini, Ivana, Bergamin, Natascha, Toffoletto, Barbara, Beltrami, Carlo A, Beltrami, Antonio P, Cesselli, Daniela, Parodi, Pier Camillo“…METHODS: To evaluate the ability to enrich stem cells, we compared, for each patient (n = 20), the standard lipoaspirate with the respective stem cell-enriched one, analyzing yield, immunophenotype and colony-forming capacity of the SVF cells as well as immunophenotype, clonogenicity and multipotency of the obtained adipose stem cells (ASCs). …”
Publicado 2015
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39042por Cheung, Y. T., Ng, T., Shwe, M., Ho, H. K., Foo, K. M., Cham, M. T., Lee, J. A., Fan, G., Tan, Y. P., Yong, W. S., Madhukumar, P., Loo, S. K., Ang, S. F., Wong, M., Chay, W. Y., Ooi, W. S., Dent, R. A., Yap, Y. S., Ng, R., Chan, A.“…Proinflammatory plasma cytokines concentrations [interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, granulocyte–macrophage colony-stimulating factor, interferon-γ and tumor necrosis factor-α] were evaluated at 3 time points (before chemotherapy, 6 and 12 weeks after chemotherapy initiation). …”
Publicado 2015
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39043por Svenningsen, Nanna B., Pérez-Pantoja, Danilo, Nikel, Pablo I., Nicolaisen, Mette H., de Lorenzo, Víctor, Nybroe, Ole“…Despite this DNA-damage mediated gene induction, we observed no increase in the mutation frequency as monitored by emergence of rifampicin-resistant colonies. CONCLUSIONS: Under conditions of extensive matric stress, we observed a direct link between matric stress, ROS formation, induction of ROS-detoxifying functions and (partial) activation of the SOS system. …”
Publicado 2015
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39044por Ophelders, Daan R. M. G., Gussenhoven, Ruth, Lammens, Martin, Küsters, Benno, Kemp, Matthew W., Newnham, John P., Payne, Matthew S., Kallapur, Suhas G., Jobe, Allan H., Zimmermann, Luc J., Kramer, Boris W., Wolfs, Tim G. A. M.“…METHODS: Pregnant ewes received an intra-amniotic injection of 10(7) colony-forming units C. albicans or saline (controls) at 3 or 5 days before preterm delivery at 0.8 of gestation (term ~ 150 days). …”
Publicado 2016
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39045por Lee, Ki Hyeong, Kim, Ji-Yeon, Lee, Moon Hee, Han, Hye Sook, Lim, Joo Han, Park, Keon Uk, Park, In Hae, Cho, Eun Kyung, Yoon, So Young, Kim, Jee Hyun, Choi, In Sil, Park, Jae Hoo, Choi, Young Jin, Kim, Hee-Jun, Jung, Kyung Hae, Kim, Si-Young, Oh, Do-Youn, Im, Seock-Ah“…PURPOSE: Pegylated granulocyte-colony-stimulating factor (G-CSF) is frequently used to prevent febrile neutropenia (FN) in patients undergoing chemotherapy with a high risk of myelosuppression. …”
Publicado 2015
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39046por Mandl, Peter, Hayer, Silvia, Karonitsch, Thomas, Scholze, Petra, Győri, David, Sykoutri, Despoina, Blüml, Stephan, Mócsai, Attila, Poór, Gyula, Huck, Sigismund, Smolen, Josef S., Redlich, Kurt“…METHODS: The presence of nAChR subunits as well as the in-vitro effects of nAChR agonists were investigated by ex vivo osteoclastogenesis assays, real-time polymerase chain reaction, Western blot and flow cytometry in murine bone marrow-derived macrophages differentiated in the presence of recombinant receptor activator of nuclear factor kappa B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). The bone phenotype of mice lacking various nAChR subunits was investigated by peripheral quantitative computed tomography and histomorphometric analysis. …”
Publicado 2016
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39047por Dolgova, Evgeniya V., Potter, Ekaterina A., Proskurina, Anastasiya S., Minkevich, Alexandra M., Chernych, Elena R., Ostanin, Alexandr A., Efremov, Yaroslav R., Bayborodin, Sergey I., Nikolin, Valeriy P., Popova, Nelly A., Kolchanov, Nikolay A., Bogachev, Sergey S.“…Thus, the internalization processes taking place in the Krebs-2 cell subpopulation have been analyzed and compared, as assayed by E. coli colony formation assay (plasmid DNA) and cytofluorescence (TAMRA-DNA). …”
Publicado 2016
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39048por Matschke, Johann, Wiebeck, Elisa, Hurst, Sebastian, Rudner, Justine, Jendrossek, Verena“…The impact of SGK1-inhibition on radiation sensitivity was determined by standard colony formation assays. The effect of GSK650394 on FA uptake was quantified by measuring intracellular accumulation of fluorescent FA (C1-BODIPY®-C12). …”
Publicado 2016
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39049por Huang, Sai, Jiang, Meng-Meng, Chen, Guo-Feng, Qian, Kun, Gao, Hong-Hao, Guan, Wei, Shi, Jin-Long, Liu, An-Qi, Liu, Jing, Wang, Bian-Hong, Li, Yong-Hui, Yu, Li“…Enhanced EYA4 expression levels inhibited cellular proliferation and suppressed cell colony formation in AML1-ETO(+) cell lines. We also found EYA4 transfection increased apoptosis of Kasumi-1 and SKNO-1 cells by 1.6-fold and 1.4-fold compared to negative control, respectively. …”
Publicado 2016
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39050por Graham, Katherine M., Kouba, Andrew J., Langhorne, Cecilia J., Marcec, Ruth M., Willard, Scott T.“…BACKGROUND: Accurate sex identification techniques are important for wildlife demographic studies and for genetic management of captive breeding colonies. Various non-invasive methods for identification of biological sex in the weakly dimorphic endangered dusky gopher frog (DGF; Lithobates sevosa) were explored to support planned recovery efforts for this species including breeding and augmentation of wild populations. …”
Publicado 2016
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39051por Cook, Deborah J., Johnstone, Jennie, Marshall, John C., Lauzier, Francois, Thabane, Lehana, Mehta, Sangeeta, Dodek, Peter M., McIntyre, Lauralyn, Pagliarello, Joe, Henderson, William, Taylor, Robert W., Cartin-Ceba, Rodrigo, Golan, Eyal, Herridge, Margaret, Wood, Gordon, Ovakim, Daniel, Karachi, Tim, Surette, Michael G., Bowdish, Dawn M. E., Lamarche, Daphnee, Verschoor, Chris P., Duan, Erick H., Heels-Ansdell, Diane, Arabi, Yaseen, Meade, Maureen“…METHODS: In a randomized blinded trial, patients expected to be mechanically ventilated for ≥72 hours were allocated to receive either 1 × 10(10) colony-forming units of Lactobacillus rhamnosus GG or placebo, twice daily. …”
Publicado 2016
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39052por Camattari, Andrea, Goh, Amelia, Yip, Lian Yee, Tan, Andy Hee Meng, Ng, Sze Wai, Tran, Anthony, Liu, Gaowen, Liachko, Ivan, Dunham, Maitreya J., Rancati, Giulia“…Normalized fluorescence from colonies carrying panARS-BFP outperformed the level of signal obtained from integrative controls by several-fold, whereas endogenous sequences, identified from the P. pastoris genome, were not as efficient in terms of protein production. …”
Publicado 2016
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39053por Labruna, Marcelo B., Nava, Santiago, Marcili, Arlei, Barbieri, Amalia R. M., Nunes, Pablo H., Horta, Mauricio C., Venzal, José M.“…These ticks were brought alive to the laboratory, and used to form two laboratory colonies (PB and PI ticks). Field-collected adults and laboratory-reared larvae were used for morphological description through light and scanning electron microscopy. …”
Publicado 2016
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39054por Loganes, Claudia, Valencic, Erica, Pin, Alessia, Marini, Elisa, Martelossi, Stefano, Naviglio, Samuele, De Leo, Luigina, Not, Tarcisio, Monasta, Lorenzo, Tommasini, Alberto, Marcuzzi, Annalisa“…RESULTS: We demonstrated that subjects with CD display a spontaneous production of inflammatory cytokines including granulocyte-colony stimulating factor (G-CSF), interleukin (IL) 6, IL8, IL10 and IL12, that was not significantly influenced by the addition of antibiotics. …”
Publicado 2016
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39055por Laith, AA, Ambak, Mohd Azmi, Hassan, Marina, Sheriff, Shahreza Md., Nadirah, Musa, Draman, Ahmad Shuhaimi, Wahab, Wahidah, Ibrahim, Wan Nurhafizah Wan, Aznan, Alia Syafiqah, Jabar, Amina, Najiah, Musa“…RESULTS: The suspected colonies of S. agalactiae identified by biochemical methods was observed as Gram-positive chained cocci, β-hemolytic, and non-motile. …”
Publicado 2017
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39056“…The function of miR-27a in gastric cancer was investigated through in vitro and in vivo assays (MTT assay, colony formation assay, flow cytometry assay, wound healing assay, migration and invasion assay, immunohistochemistry (IHC), immunofluorescence (IF) and Western blot). …”
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39057por Liu, Qiang, Cui, Xiang, Yu, Xi, Bian, Bai-Shi-Jiao, Qian, Feng, Hu, Xu-gang, Ji, Cheng-dong, Yang, Lang, Ren, Yong, Cui, Wei, Zhang, Xia, Zhang, Peng, Wang, Ji Ming, Cui, You-hong, Bian, Xiu-wu“…METHODS: ESCC cells with CR-1 (high) or CR-1(low) were obtained by flow cytometry then their self-renewal capability and tumorigenicity were compared by colony and limiting dilution sphere formation analysis in vitro and xenograft in nude mice in vivo, respectively. …”
Publicado 2017
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39058por Ramos-Silva, Alberto, Tavares-Carreón, Faviola, Figueroa, Mario, De la Torre-Zavala, Susana, Gastelum-Arellanez, Argel, Rodríguez-García, Aída, Galán-Wong, Luis J., Avilés-Arnaut, Hamlet“…The extract caused a significant reduction of cell motility and colony formation on all evaluated cancer cell lines. …”
Publicado 2017
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39059por Yu, Zhibin, Sun, Yingnan, She, Xiaoling, Wang, Zeyou, Chen, Shuai, Deng, Zhiyong, Zhang, Yan, Liu, Qiang, Liu, Qing, Zhao, Chunhua, Li, Peiyao, Liu, Changhong, Feng, Jianbo, Fu, Haijuan, Li, Guiyuan, Wu, Minghua“…Flow cytometry was performed to assess the effect of SIX3 on cell cycle distribution. Colony formation, EdU incorporation, transwell, and intracranial xenograft assays were performed to demonstrate the effect of SIX3 on the malignant phenotype of astrocytoma cells. …”
Publicado 2017
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39060por Zhang, Jiqin, Song, Fei, Zhao, Xiaojing, Jiang, Hua, Wu, Xiuqi, Wang, Biao, Zhou, Min, Tian, Mi, Shi, Bizhi, Wang, Huamao, Jia, Yuanhui, Wang, Hai, Pan, Xiaorong, Li, Zonghai“…The growth of lung cancer was detected by CCK-8 assay, Annexin V/PI staining, colony formation assay and subcutaneous xenograft assay. …”
Publicado 2017
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