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39181“…Cell viability, migration and invasion were analyzed using cell counting kit-8, colony formation and transwell assay, respectively. …”
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39182por Macedo-Silva, Catarina, Miranda-Gonçalves, Vera, Lameirinhas, Ana, Lencart, Joana, Pereira, Alexandre, Lobo, João, Guimarães, Rita, Martins, Ana Teresa, Henrique, Rui, Bravo, Isabel, Jerónimo, Carmen“…The effectiveness of RT upon ESCC cells under hypoxic conditions was assessed by colony formation assay. KDM3A/KDM6B expression, and respective H3K9me2 and H3K27me3 target marks, were evaluated by RT-qPCR, Western blot, and immunofluorescence. …”
Publicado 2020
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39183por Gao, Lei, Zhang, Yanqi, Wang, Sanbin, Kong, Peiyan, Su, Yi, Hu, Jiong, Jiang, Ming, Bai, Hai, Lang, Tao, Wang, Jishi, Liu, Li, Yang, Tonghua, Huang, Xiaobing, Liu, Fang, Lou, Shifeng, Liu, Yao, Zhang, Cheng, Liu, Hong, Gao, Li, Liu, Jia, Zhu, Lidan, Wen, Qin, Chen, Ting, Wang, Ping, Rao, Jun, Mao, Min, Wang, Cunbang, Duan, Xianlin, Luo, Le, Peng, Xiangui, Cassady, Kaniel, Zhong, Jiang F., Zhang, Xi“…The aim of this study was to explore the effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) combined with minimal-dose decitabine (Dec) on the prevention of HR-AML relapse after allo-HSCT. …”
Publicado 2020
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39184“…Cell counting kit-8, plate colony formation and transwell assays were used to analyse the cells biological behaviour.. …”
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39185por Zhang, Dongdong, Li, Sheng, Hou, Liyan, Jing, Lu, Ruan, Zhengzheng, Peng, Bingjie, Zhang, Xiaomeng, Hong, Jau-Shyong, Zhao, Jie, Wang, Qingshan“…METHODS: To evaluate the role of microglia in rotenone-induced cognitive deficits, PLX3397, an inhibitor of colony-stimulating factor 1 receptor, and minocycline, a widely used antibiotic, were used to deplete or inactivate microglia, respectively. …”
Publicado 2021
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39186“…Mechanically, cell proliferation was monitored using MTT assay, cell colony formation assay and EdU assay. Luciferase reporter assay was used to identify the downstream target gene of miR-492. …”
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39187por Heinz, Rebecca, Brandenburg, Susan, Nieminen-Kelhä, Melina, Kremenetskaia, Irina, Boehm-Sturm, Philipp, Vajkoczy, Peter, Schneider, Ulf C.“…METHODS: 7T-MRI-controlled SAH was induced by endovascular perforation in four groups of C57Bl/6 mice: (i) Sham-operation, (ii) SAH naïve, (iii) SAH followed by inflammatory preconditioning (LPS intraperitoneally), and (iv) SAH followed by pharmacological microglia deactivation (colony-stimulating factor-1 receptor-antagonist PLX3397 intraperitoneally). …”
Publicado 2021
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39188por Hua, Yunqi, Wang, Hailong, Wang, Haizhen, Wu, Xiangming, Yang, Li, Wang, Chenlin, Li, Xi, Jin, Yunjian, Li, Min, Wang, Lina, Dong, Changcheng, Yin, Fangrui“…Cell Counting Kit-8 (CCK-8) assay and colony formation assay were employed for cell proliferation. …”
Publicado 2021
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39189por Wang, Yuanyong, Wo, Yang, Lu, Tong, Sun, Xiao, Liu, Ao, Dong, Yanting, Du, Wenxing, Su, Wenhao, Huang, Zhangfeng, Jiao, Wenjie“…METHODS: The circ-AASDH, miR-140-3p and E2F transcription factor 7 (E2F7) mRNA expression levels were detected via qRT-PCR. CCK-8 and colony formation assay were used to evaluate the ability of cell proliferation. …”
Publicado 2021
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39190por Qu, Xiaoyan, Yu, Bing, Zhu, Mengmei, Li, Xiaomei, Ma, Lishan, Liu, Chuyin, Zhang, Yixing, Cheng, Zhongping“…MATERIALS AND METHODS: The effect of anti-ovarian cancer HeyA8 cells was analyzed by CCK8 and colony formation assay. The mechanism of sinomenine anti-ovarian cancer was explored via high throughput RNA-seq, and then the target mRNA and protein expression were verified by real-time PCR and Western blot, respectively. …”
Publicado 2021
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39191por Liu, Tao, Han, Xiujuan, Zheng, Shutao, Liu, Qing, Tuerxun, Aerziguli, Zhang, Qiqi, Yang, Lifei, Lu, Xiaomei“…MTT, flow cytometry, Transwell migration, scratch wound-healing and colony formation assays were performed to assay the combined effect of knock-out CALM1 and EGFR inhibitor on ESCC cell proliferation and migration. …”
Publicado 2021
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39192“…Cell proliferation was detected by Cell counting Kit-8 (CCK-8) and colony formation assays. Flow cytometry was used for determining cell cycle and cell apoptosis. …”
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39193por Shan, Guang, Zhou, Xike, Gu, Juan, Zhou, Daoping, Cheng, Wei, Wu, Huaiguo, Wang, Yueping, Tang, Tian, Wang, Xuedong“…METHODS: Transwell, MTT, flow cytometry and colony formation assays were applied to assess the effects of CAF-derived exosomes on bladder cancer cell metastasis, epithelial-mesenchymal transition (EMT) and chemosensitivity. …”
Publicado 2021
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39194por Tesei, Anna, Arienti, Chiara, Bossi, Gianluca, Santi, Spartaco, De Santis, Ilaria, Bevilacqua, Alessandro, Zanoni, Michele, Pignatta, Sara, Cortesi, Michela, Zamagni, Alice, Storci, Gianluca, Bonafè, Massimiliano, Sarnelli, Anna, Romeo, Antonino, Cavallo, Carola, Bartolazzi, Armando, Rossi, Stefania, Soriani, Antonella, Strigari, Lidia“…IR-A549 EVs also hamper the colony-forming capability of recipient NIR A549 cells, induce senescent phenotype, nuclear expression of DNA:RNA hybrids, and M1 macrophage polarization. …”
Publicado 2021
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39195“…Cell growth, metastasis, and apoptosis were investigated via colony formation, 5-ethynyl-2′-deoxyuridine (EdU) staining, MTT, flow cytometry, Western blotting, and transwell analyses. …”
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39196por Yang, Chih-Yi, Lu, Rita Jui-Hsien, Lee, Ming-Kang, Hsiao, Felix Shih-Hsian, Yen, Ya-Ping, Cheng, Chun-Chun, Hsu, Pu-Sheng, Tsai, Yi-Tzang, Chen, Shih-Kuo, Liu, I-Hsuan, Chen, Pao-Yang, Lin, Shau-Ping“…Here, we report the reduced colony forming ability and impaired in vitro osteogenesis in Dnmt3l-knockout-mice-derived MSCs (Dnmt3l KO MSCs). …”
Publicado 2021
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39197por Yue, Qianyu, Xu, Yanyan, Deng, Xiaoli, Wang, Shenglan, Qiu, Jingman, Qian, Baojiang, Zhang, Yunhui“…Cell proliferation, apoptosis, cell cycle process, migration and invasion were determined using cell counting kit 8 (CCK8) assay, colony formation assay, flow cytometry, wound healing assay and transwell assay. …”
Publicado 2021
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39198“…To untangle the relative importance of ecological determinants of predator fine-scale habitat selection, we studied simultaneously several spatial, temporal, and behavioural predictors of habitat selection in territorial arctic foxes (Vulpes lagopus) living within a Greater snow goose (Anser caerulescens atlantica) colony during the reproductive season. METHODS: Using GPS locations collected at 4-min intervals and behavioural state classification (active and resting), we quantified how foxes modulate state-specific habitat selection in response to territory edges, den proximity, prey distribution, and habitats. …”
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39199por Wu, Shu-I, Wu, Chien-Chen, Tsai, Pei-Joung, Cheng, Li-Hao, Hsu, Chih-Chieh, Shan, Ian-Kai, Chan, Po-Ying, Lin, Ting-Wei, Ko, Chih-Jung, Chen, Wan-Lin, Tsai, Ying-Chieh“…Participants were asked to take two capsules containing PS128(TM) powder, equivalent to 20 billion colony-forming units, daily. Self-report measures, such as the Job Stress Scale, Visual Analog Scale of Stress, the Insomnia Severity Index, the State and Trait Anxiety Index, the Questionnaire for Emotional Trait and State, the Patient Health Questionnaire, the Quality of Life Enjoyment and Satisfaction Questionnaire, and Gastrointestinal Severity Index were compared at baseline and at the end of the trial period. …”
Publicado 2021
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39200por Nekliudova, U. A., Schwaha, T. F., Kotenko, O. N., Gruber, D., Cyran, N., Ostrovsky, A. N.“…Although they are structurally less complex, invertebrate placentae are much more diverse in their origin, development and position. Aquatic colonial suspension-feeders from the phylum Bryozoa acquired placental analogues multiple times, representing an outstanding example of their structural diversity and evolution. …”
Publicado 2021
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