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39641por Grassotti, Tiela Trapp, de Angelis Zvoboda, Dejoara, da Fontoura Xavier Costa, Letícia, de Araújo, Alberto Jorge Gomes, Pereira, Rebeca Inhoque, Soares, Renata Oliveira, Wagner, Paulo Guilherme Carniel, Frazzon, Jeverson, Frazzon, Ana Paula Guedes“…A total of 24 swabs/fecal samples were collected, including 19 from wild monkeys living in two forest fragments [São Sebastião do Caí (SSC) and Santa Cruz do Sul (SCS)], and five in captive [Parque Zoológico da Fundação Zoobotânica (ZOO)], between August 2016 and November 2017. Fifteen colonies were randomly selected from each sample. Enterococci were identified by MALDI-TOF, tested for susceptibility to 12 antibiotics; and screened for tet(S), tet(M), tet(L), msrC, and erm(B) genes by PCR. …”
Publicado 2018
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39642por Hooghiemstra, Henry, Olijhoek, Thomas, Hoogland, Menno, Prins, Maarten, van Geel, Bas, Donders, Timme, Gosling, William, Hofman, Corinne“…AD 1650 reflects intensive forest clearing, suggesting that small-scale Pre-Colonial practice of crop cultivation became replaced by large-scale agriculture on the moist and nutrient rich soils along the Yaque River. …”
Publicado 2018
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39643por Caudell, Mark A, Mair, Colette, Subbiah, Murugan, Matthews, Louise, Quinlan, Robert J, Quinlan, Marsha B, Zadoks, Ruth, Keyyu, Julius, Call, Douglas R“…Samples were processed and plated onto MacConkey agar plates, then presumptive E coli isolates were identified on the basis of colony morphology. Susceptibility of isolates was then tested against a panel of nine antimicrobials (ampicillin, ceftazidime, chloramphenicol, ciprofloxacin, kanamycin, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim) via a breakpoint assay. …”
Publicado 2018
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39644por Liu, Nian, Wang, Kuan Song, Qi, Min, Zhou, Ying Jun, Zeng, Guang Yao, Tao, Juan, Zhou, Jian Da, Zhang, Jiang Lin, Chen, Xiang, Peng, Cong“…METHODS: We first examined the effects of VB1 (vitexin compound 1) on cell viability by CCK-8 (cell counting kit) and Colony Formation Assay; And then, we analyzed the apoptosis and cell cycle by flow cytometry, verified apoptosis by Immunoblotting. …”
Publicado 2018
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39645por Meki, Irene K., Kariithi, Henry M., Parker, Andrew G., Vreysen, Marc J B, Ros, Vera I D, Vlak, Just M, van Oers, Monique M, Abd-Alla, Adly M. M.“…GpSGHV infections are usually asymptomatic, but unknown factors can result to a switch to acute symptomatic infection, which is characterized by the salivary gland hypertrophy (SGH) syndrome associated with decreased fecundity that can ultimately lead to a colony collapse. It is uncertain how GpSGHV is maintained amongst Glossina spp. populations but RNA interference (RNAi) machinery, a conserved antiviral defense in insects, is hypothesized to be amongst the host’s mechanisms to maintain the GpSGHV in asymptomatic (persistent or latent) infection state. …”
Publicado 2018
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39646por De La Cadena, Elsa Piedad, Mojica, Maria Fernanda, Hernandez, Cristhian, Correa, Adriana, Pallares, Christian, Villegas, Maria Virginia“…DD may be useful for Eco and Ent, when scattered colonies within inhibition zones are ignored. Results also show high rates of FOS susceptibility among clinical isolates. …”
Publicado 2018
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39647por Wang, Marie, Lee, Vivian, Greenhow, Tara, Beck, Jimmy, Bendel-Stenzel, Michael, Hames, Nicole, McDaniel, Corrie, King, Erin, Sherry, Whitney, Parmar, Deepika, Patrizi, Sara, Srinivas, Nivedita, Schroeder, Alan“…ESC-R UTI was defined as having a urinalysis with positive leukocyte esterase or >5 white cells per high-power field and urine culture with ≥50,000 colony-forming units per milliliter of E. coli or Klebsiella spp. nonsusceptible to ceftriaxone. …”
Publicado 2018
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39648por Palmieri, Lola-Jade, Rainteau, Dominique, Sokol, Harry, Beaugerie, Laurent, Dior, Marie, Coffin, Benoit, Humbert, Lydie, Eguether, Thibaut, Bado, André, Hoys, Sandra, Janoir, Claire, Duboc, Henri“…Results: In vitro, UDCA inhibited germination and growth of C. difficile at 0.05 and 0.1%, competing with 0.1% TCA (with 0.1%: 0.05% ± 0.05% colony forming unit versus 100% ± 0%, P < 0.0001). …”
Publicado 2018
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39649por Surendranathan, Ajenthan, Su, Li, Mak, Elijah, Passamonti, Luca, Hong, Young T, Arnold, Robert, Vázquez Rodríguez, Patricia, Bevan-Jones, William R, Brain, Susannah A E, Fryer, Tim D, Aigbirhio, Franklin I, Rowe, James B, O’Brien, John T“…Peripheral blood inflammatory cytokines were also measured in all subjects, as well as in an additional 10 controls, using the Mesoscale Human Cytokine 36 plex panel and additional assays for high sensitivity c-reactive protein, tumour necrosis factor receptor 1, IL-34, YKL-40 (chitinase-3-like protein 1) and colony stimulating factor 1. To test for the presence of in vivo amyloid, (11)C-Pittsburgh compound B PET imaging was also performed in 16 of the dementia with Lewy body participants. …”
Publicado 2018
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39650por Zhang, Chengshuo, Chen, Baomin, Jiao, Ao, Li, Feng, Sun, Ning, Zhang, Guoqing, Zhang, Jialin“…Cell proliferation was investigated using MTS, EdU, colony formation assays, and xenograft animal experiments, and the cell invasion capacity was evaluated using the transwell assay. …”
Publicado 2018
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39651por Pomraning, Kyle R., Bredeweg, Erin L., Kerkhoven, Eduard J., Barry, Kerrie, Haridas, Sajeet, Hundley, Hope, LaButti, Kurt, Lipzen, Anna, Yan, Mi, Magnuson, Jon K., Simmons, Blake A., Grigoriev, Igor V., Nielsen, Jens, Baker, Scott E.“…All the smooth mutants identified, so named because of their colony morphology, exhibit independent loss of DNA at a repetitive locus made up of interspersed ribosomal DNA and short 10- to 40-mer telomere-like repeats. …”
Publicado 2018
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39652por Gargis, A. S., McLaughlin, H. P., Conley, A. B., Lascols, C., Michel, P. A., Gee, J. E., Marston, C. K., Kolton, C. B., Rodriguez-R, L. M., Hoffmaster, A. R., Weigel, L. M., Sue, D.“…An unexpectedly small colony size, a reduced growth rate, and undetectable β-lactamase activity levels (culture supernatant and cell lysate) were observed in this PEN-S strain. …”
Publicado 2018
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39653por Miyazaki, Yusuke, Nakayamada, Shingo, Kubo, Satoshi, Nakano, Kazuhisa, Iwata, Shigeru, Miyagawa, Ippei, Ma, Xiaoxue, Trimova, Gulzhan, Sakata, Kei, Tanaka, Yoshiya“…Co-culture of Th22 cells, which were sorted from peripheral blood, with monocytes in the presence of macrophage colony-stimulating factor and receptor activator of nuclear factor (NF)-κB ligand induced osteoclasts formation more efficiently than that of either Th1 cells or Th17 cells. …”
Publicado 2018
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39654por Sikorska, Justyna, Gaweł, Damian, Domek, Hanna, Rudzińska, Magdalena, Czarnocka, Barbara“…Then, we tested the motility and invasiveness of these cells (using scratch test and Transwell assay), their growth capacities F(cell cycle analysis, viability, clonogenic activity) and apoptosis assays), adhesion-independent colony-formation capacities, as well as the effect of PDPN silencing on MMPs expression and activity (zymography). …”
Publicado 2019
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39655por Yin, Yu, Xu, Lingfan, Chang, Yan, Zeng, Tao, Chen, Xufeng, Wang, Aifeng, Groth, Jeff, Foo, Wen-Chi, Liang, Chaozhao, Hu, Hailiang, Huang, Jiaoti“…Migration, cell proliferation and colony formation assays were used to measure the cellular response after overexpressing N-Myc or perturbing the miR-421/ATM pathway. …”
Publicado 2019
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39656por Amit, Chatterjee, Muralikumar, Shalini, Janaki, Sargunam, Lakshmipathy, Meena, Therese, Kulandai Lily, Umashankar, Vetrivel, Padmanabhan, Prema, Narayanan, Janakiraman“…Insilico prediction of peptides showed it to have antifungal activity which was further validated using colony forming assay and time killing kinetics. The duration of antifungal activity of peptide was improved using gelatin hydrogel through sustained delivery. …”
Publicado 2019
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39657por Bietrix, Jacques, Kolenda, Camille, Sapin, Anaïs, Haenni, Marisa, Madec, Jean-Yves, Bes, Michèle, Dupieux, Céline, Tasse, Jason, Laurent, Fréderic“…After pre-enrichment of swabs and milk, they were subcultured on MSSA/MRSA chromogenic selective agar plates. S. aureus colonies were tested with a multiplex PCR to detect the mecA and mecC genes. …”
Publicado 2019
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39658por Emerson, Sam R, Sciarrillo, Christina M, Kurti, Stephanie P, Emerson, Emily M, Rosenkranz, Sara K“…IL-8, VEGF-A, VEGF-C, and heparin-binding epidermal growth factor–like growth factor significantly increased, whereas granulocyte-macrophage colony-stimulating factor, interferon-γ, IL-1β, IL-5, IL-10, IL-12, IL-13, IL-17A, IL-23, TNF-α, leptin, angiopoietin-2, and follistatin significantly decreased after HFM consumption (P’s < 0.05). …”
Publicado 2018
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39659por Menchinelli, Giulia, Liotti, Flora Marzia, Fiori, Barbara, De Angelis, Giulia, D'Inzeo, Tiziana, Giordano, Liliana, Posteraro, Brunella, Sabbatucci, Michela, Sanguinetti, Maurizio, Spanu, Teresa“…In this study, we assessed TTD for 330 clinically relevant species including 14 Gram-positive, 14 Gram-negative, and 5 yeast isolates in spiked human blood samples that were tested in parallel with VIRTUO BACT/ALERT® 3D (BTA3D) and BACTEC™ FX (BACTEC) systems. We inoculated 30 colony-forming unit (CFU) from each microbial suspension into BACT/ALERT® Plus or BACTEC™ Plus (aerobic/anaerobic or pediatric) BC bottles, and we used two different blood volumes to simulate, respectively, the BCs collected from adult and pediatric patients. …”
Publicado 2019
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39660por Al Mahtab, Mamun, MF Akbar, Sheikh, Begum, Masuda, Islam, Md. A, Rahim, Md. A, M Noor-E-Alam, Sheikh, Alam, Md. A, A Khondaker, Faiz, L Moben, Ahmed, Mohsena, Masuda, Khan, Md. Sakirul I, Huq, Md. Z, Munshi, Swati, Hoque, Ashraful, Haque, Sheikh A“…MATERIALS AND METHODS: A total of 34 patients received granulocyte colony-stimulating factor at a dose of 30 IU, daily for 2 to 11 days to upregulate the numbers of white blood cells and stem cells. …”
Publicado 2018
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