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40041por Xu, Teng, Yang, Yuemei, Chen, Zhihong, Wang, Jinsong, Wang, Xiaolei, Zheng, Yang, Wang, Chao, Wang, Yachen, Zhu, Zaiou, Ding, Xu, Zhou, Junbo, Li, Gang, Zhang, Hongchuang, Zhang, Wei, Wu, Yunong, Song, Xiaomeng“…Half-maximal inhibitory concentration (IC50) examination, colony formation assays and flow cytometry assays were conducted to examine the role of TNFAIP2 in vitro, while xenograft models in nude mice and 4-nitroquinoline N-oxide (4NQO)-induced HNSCC models in C57BL/6 mice were adopted to verify the effect of TNFAIP2 in vivo. …”
Publicado 2023
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40042por Carpenter, Elizabeth Lee, Van Decar, Spencer, Adams, Alexandra M, O’Shea, Anne E, McCarthy, Patrick, Chick, Robert Connor, Clifton, Guy Travis, Vreeland, Timothy, Valdera, Franklin A, Tiwari, Ankur, Hale, Diane, Kemp Bohan, Phillip, Hickerson, Annelies, Smolinsky, Todd, Thomas, Katryna, Cindass, Jessica, Hyngstrom, John, Berger, Adam C, Jakub, James, Sussman, Jeffrey J, Shaheen, Montaser F, Yu, Xianzhong, Wagner, Thomas E, Faries, Mark, Peoples, George E“…Here we report the 36-month prespecified analyses of this prospective, randomized, double-blind trial investigating the ability of the TLPO and TLPLDC (±granulocyte-colony stimulating factor (G-CSF)) vaccines to prevent melanoma recurrence in high-risk patients. …”
Publicado 2023
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40043por Ali, Hassan R. W., Suliman, Salwa, Osman, Tarig Al-Hadi, Carrasco, Manuel, Bruland, Ove, Costea, Daniela-Elena, Ræder, Helge, Mustafa, Kamal“…Morphologically, all iPS generated from fibroblasts formed tight colonies surrounded by a reflective “whitish” outer rim, typical for iPS. …”
Publicado 2023
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40044por Moutafi, Myrto, Koliou, Georgia-Angeliki, Papaxoinis, George, Economopoulou, Panagiota, Kotsantis, Ioannis, Gkotzamanidou, Maria, Anastasiou, Maria, Pectasides, Dimitrios, Kyrodimos, Efthymios, Delides, Alexander, Giotakis, Evangelos, Papadimitriou, Nikolaos G., Panayiotides, Ioannis G., Perisanidis, Christos, Fernandez, Aileen I., Xirou, Vasiliki, Poulios, Christos, Gagari, Eleni, Yaghoobi, Vesal, Gavrielatou, Niki, Shafi, Saba, Aung, Thazin Nwe, Kougioumtzopoulou, Andromachi, Kouloulias, Vassilis, Palialexis, Konstantinos, Gkolfinopoulos, Stavros, Strati, Areti, Lianidou, Evi, Fountzilas, George, Rimm, David L., Foukas, Periklis G., Psyrri, Amanda“…Expression of CD163 and colony-stimulating factor 1 receptor (CSF1R) genes, markers of M2 macrophages, increased significantly posttreatment whereas the expression of CD80, a marker of M1 macrophages, decreased. …”
Publicado 2023
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40045por Ye, Mujie, Chen, Jinhao, Lu, Feiyu, Zhao, Minghui, Wu, Suwen, Hu, Chunhua, Yu, Ping, Kan, Jingbao, Bai, Jianan, Tian, Ye, Tang, Qiyun“…The effects of FTO and ALKBH5 on cell proliferation were examined using CCK-8, colony formation, and EdU assays, and the effects on cell migration and invasion were tested using a transwell assay. m6A RNA immunoprecipitation (MeRIP) and RNA-seq was used to explore downstream target gene. …”
Publicado 2023
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40046por Yang, Yi-Chieh, Lin, Yung-Wei, Lee, Wei-Jiunn, Lai, Feng-Ru, Ho, Kuo-Hao, Chu, Chih-Ying, Hua, Kuo-Tai, Chen, Ji-Qing, Tung, Min-Che, Hsiao, Michael, Wen, Yu-Ching, Chien, Ming-Hsien“…Cell loss-of-function and gain-of-function, colony-formation, anoikis, and transwell assays, and an orthotopic bioluminescent xenograft model were conducted to determine the functional role of KRSP in ccRCC progression. …”
Publicado 2023
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40047“…Cell cycle was measured by flow cytometry. CCK-8, plate colony formation and EdU assays were performed to assess the cell proliferation. …”
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40048por Liu, Yun, Cao, Jia, Yang, Qi, Zhu, Linqi, Zhao, Wenjun, Wang, Xiuping, Yao, Jun, Zhou, Yong, Shao, Shihe“…The function of circRNA_15430 in GC cells were examined by using colony formation, cell counting kit-8 (CCK-8) and Transwell assays, flow cytometry and laser scanning confocal microscopy. …”
Publicado 2023
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40049“…METHODS: Different expression of VSIG2 in PDAC tissues and cells were detected by bioinformatic analysis, immunohistochemistry, real-time quantitative PCR as well as western blotting. CCK-8, colony formation, Transwell assay, and scratch experiment were utilized to assess proliferation, invasion and migration properties of PDAC cells. …”
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40050por Scridel, Davide, Pirrello, Simone, Imperio, Simona, Cecere, Jacopo G., Albanese, Giuseppe, Andreotti, Alessandro, Arveda, Giovanni, Borghesi, Fabrizio, La Gioia, Giuseppe, Massa, Luisanna, Mengoni, Chiara, Micheloni, Pierfrancesco, Mucci, Nadia, Nardelli, Riccardo, Nissardi, Sergio, Volponi, Stefano, Zucca, Carla, Serra, Lorenzo“…METHODS: We investigated these decision making-processes for 40 juvenile greater flamingos Phoenicopterus roseus fledged in three Mediterranean colonies and equipped with GPS-GSM devices. RESULTS: Contrary to the body size and the dominance hypotheses, juveniles in better body condition were more likely to migrate than those in worse conditions, which opted for a residence strategy. …”
Publicado 2023
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40051por Ma, Shuoshuo, Ma, Yang, Qi, Feiyu, Lei, Jiasheng, Chen, Fangfang, Sun, Wanliang, Wang, Dongdong, Zhou, Shuo, Liu, Zhong, Lu, Zheng, Zhang, Dengyong“…The proliferation, migration, and invasion of CCA cells were assessed using Cell Counting Kit-8, colony formation, 5-ethynyl-2′-deoxyuridine DNA synthesis, and transwell assays. …”
Publicado 2023
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40052por Liu, Tao, Fan, Meng-Qi, Xie, Xiao-Xiao, Shu, Qi-Peng, Du, Xue-Hua, Qi, Lin-Zhi, Zhang, Xiao-Dong, Zhang, Ming-Hui, Shan, Guang, Du, Run-Lei, Li, Shang-Ze“…Then, we performed CCK-8, colony formation, soft agar and Transwell migration assays to determine the impact of the UCHL3 gene on cell phenotype. …”
Publicado 2023
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40053“…In functional tests, cell counting kit-8 (CCK-8) assay, colony formation assay, wound healing assay, transwell invasion assay, Immunofluorescence (IF) assay and immunohistochemical (IHC) assay were performed. …”
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40054por Sabbah, Rawan, Saadi, Sahar, Shahar-Gabay, Tal, Gerassy, Shiran, Yehudai-Resheff, Shlomit, Zuckerman, Tsila“…ELISA, FACS analysis, colony forming unit assay, whole exome sequencing and real-time qPCR were employed to assess the differentiation capacity, genetic status, gene expression and function of these cell fractions. …”
Publicado 2023
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40055“…Accordingly, we observed that restoration of PRDM15 expression could rescue the deficiency of CCA cell proliferation/colony formation induced by METTL16 depletion. Our subsequent analyses revealed that METTL16-PRDM15 signaling regulated the expression of FGFR4 in CCA cells. …”
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40056“…Cell proliferation, migration, invasion, and apoptosis abilities were assessed with BrdU and colony formation assay, wound-healing assay, and flow cytometry, respectively. …”
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40057por Di Donato, Marzia, Di Zazzo, Erika, Salvati, Annamaria, Sorrentino, Carmela, Giurato, Giorgio, Fiore, Donatella, Proto, Maria Chiara, Rienzo, Monica, Casamassimi, Amelia, Gazzerro, Patrizia, Bifulco, Maurizio, Castoria, Gabriella, Weisz, Alessandro, Nassa, Giovanni, Abbondanza, Ciro“…Noteworthy, the forced RIZ2 expression increased cell viability, growth, colony formation, migration and organoid formation. …”
Publicado 2023
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40058por Mazzella, Matteo, Walker, Keegan, Cormier, Christina, Kapanowski, Michael, Ishmakej, Albi, Saifee, Azeem, Govind, Yashvardhan, Chaudhry, G. Rasul“…To evaluate LP and HP pMSCs, we examined their physical characteristics, cell surface markers, growth rate, colony-forming ability, BrdU assays for proliferation, telomerase activity, and potential to differentiate into three lineages. …”
Publicado 2023
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40059“…Meta results showed that PDT was superior to nystatin in reducing the number of oral candida colonies in the palate of patients MD = -0.87, 95%CI = (-1.52,-0.23), P = 0.008, the difference was statistically significant, and the denture site MD = -1.03, 95%CI = (-2.21, -0.15), P = 0.09, the difference was not statistically significant; compared with the efficacy of fluconazole, RR = 1.01, 95%CI = (0.56,1.83), P = 0.96; compared with miconazole RR = 0.55, 95%CI = (0.38, 0.81), P = 0.002; PDT combined with nystatin RR = 1.27, 95%CI = (1.06, 1.52), P = 0.01; recurrence rate RR = 0.28, 95%CI = (0.09, 0.88), P = 0.03. …”
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40060“…Then, the oncogenic functions of G3BP1 dependent on the SPOP/ERα axis were determined by CCK8 cell proliferation assay, colony formation assay and cell migration assay. Finally, we established the EC cells treated or untreated with fulvestrant, exploring the possibility of fulvestrant combined with the reduction of G3BP1 to improve the efficacy of fulvestrant. …”
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