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40081por Lipson, Evan J, Sharfman, William H, Chen, Shuming, McMiller, Tracee L, Pritchard, Theresa S, Salas, January T, Sartorius-Mergenthaler, Susan, Freed, Irwin, Ravi, Sowmya, Wang, Hao, Luber, Brandon, Sproul, Janice Davis, Taube, Janis M, Pardoll, Drew M, Topalian, Suzanne L“…This first-in-human study investigated the safety, tolerability and immunologic correlates of Melanoma GVAX, a lethally irradiated granulocyte–macrophage colony stimulating factor (GM-CSF)-secreting allogeneic whole-cell melanoma vaccine, administered in the adjuvant setting. …”
Publicado 2015
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40082por Bjørklund, Sunniva Stordal, Kristensen, Vessela N., Seiler, Michael, Kumar, Surendra, Alnæs, Grethe I. Grenaker, Ming, Yao, Kerrigan, John, Naume, Bjørn, Sachidanandam, Ravi, Bhanot, Gyan, Børresen-Dale, Anne-Lise, Ganesan, Shridar“…Knock down by short hairpin RNA (shRNA) and colony formation assays were used to determine whether ACOX2-i9 expression would influence cellular fitness. …”
Publicado 2015
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40083“…With combination of these two compounds there was a significant decrease in these two proteins which might further resulted in significant growth inhibition, apoptosis and cell cycle arrest in HCC1806 and MDA-MB-157 breast cancer cells, while there was no significant effect on cellular viability, colony forming potential, morphology or apoptosis in control MCF10A breast epithelial cells. …”
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40084por Madalli, Shimona, Beyrau, Martina, Whiteford, James, Duchene, Johan, Singh Nandhra, Inderpal, Patel, Nimesh S. A., Motwani, Madhur P., Gilroy, Derek W., Thiemermann, Christoph, Nourshargh, Sussan, Scotland, Ramona S.“…Cxcl5 treatment of bone marrow neutrophils in vitro caused substantial induction of neutrophil-mobilizing cytokine granulocyte colony-stimulating factor (GCSF) and expression of β2 integrin that accounts for sexual dimorphism in circulating neutrophil populations in I/R. …”
Publicado 2015
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40085por Marayati, Bahjat Fadi, Schal, Coby, Ponnusamy, Loganathan, Apperson, Charles S., Rowland, Tobin E., Wasserberg, Gideon“…Six treatments were placed on separate filter paper discs at the bottom of the jar: 2(nd)/3(rd) larval instar medium, 4(th) larval instar/pupae medium, frass from expired colonies, larval food (aged rabbit chow and rabbit feces mix), rabbit feces, and a solvent (water) control. …”
Publicado 2015
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40086por Sun, Hai-yan, Liu, Bei-bei, Hu, Jian-yang, Xu, Li-jia, Chan, Shun-wan, Chan, Chi-on, Mok, Daniel K. W., Zhang, Dong-mei, Ye, Wen-cai, Chen, Si-bao“…Their anticancer effects on several human cancer cell lines, MCF-7, HepG2, HepG2/ADM, HeLa, and PC3, and normal human mammary epithelial cells MCF10A were investigated by colony formation and MTT assays. Morphological analysis of apoptosis induction was performed by acridine orange/ethidium bromide dual-staining and Hoechst 33258 nuclear staining. …”
Publicado 2016
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40087por Chang, Shwu-Fen, Li, Huai-Ci, Huang, Yu-Pei, Tasi, Wen-Ju, Chou, Yuan-Yi, Lu, Shao-Chun“…BACKGROUND: Granulocyte-colony stimulating factor (G-CSF) is a major regulator of the production and survival of neutrophils. …”
Publicado 2016
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40088por Ranki, Tuuli, Pesonen, Sari, Hemminki, Akseli, Partanen, Kaarina, Kairemo, Kalevi, Alanko, Tuomo, Lundin, Johan, Linder, Nina, Turkki, Riku, Ristimäki, Ari, Jäger, Elke, Karbach, Julia, Wahle, Claudia, Kankainen, Matti, Backman, Charlotta, von Euler, Mikael, Haavisto, Elina, Hakonen, Tiina, Heiskanen, Raita, Jaderberg, Magnus, Juhila, Juuso, Priha, Petri, Suoranta, Laura, Vassilev, Lotta, Vuolanto, Antti, Joensuu, Timo“…BACKGROUND: We conducted a phase I study with a granulocyte macrophage colony stimulating factor (GMCSF)-expressing oncolytic adenovirus, ONCOS-102, in patients with solid tumors refractory to available treatments. …”
Publicado 2016
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40089por El-Sayed, Karim M Fawzy, Paris, Sebastian, Graetz, Christian, Kassem, Neemat, Mekhemar, Mohamed, Ungefroren, Hendrick, Fändrich, Fred, Dörfer, Christof“…Subsequently, the MACS(+) and MACS(−) cell fractions were characterized by flow cytometry for expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146/MUC18 and STRO-1. Colony-forming unit (CFU) and multilineage differentiation potential were assayed for both cell fractions. …”
Publicado 2015
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40090por Xu, Dan-dan, Zhou, Peng-jun, Wang, Ying, Zhang, Li, Fu, Wu-yu, Ruan, Bi-bo, Xu, Hai-peng, Hu, Chao-zhi, Tian, Lu, Qin, Jin-hong, Wang, Sheng, Wang, Xiao, Li, Yi-cheng, Liu, Qiu-ying, Ren, Zhe, Zhang, Rong, Wang, Yi-fei“…Sphere formation assay and colony formation assays were employed to analyze the relationship between STAT3 and miR-181b. …”
Publicado 2016
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40091por Barthow, Christine, Wickens, Kristin, Stanley, Thorsten, Mitchell, Edwin A., Maude, Robyn, Abels, Peter, Purdie, Gordon, Murphy, Rinki, Stone, Peter, Kang, Janice, Hood, Fiona, Rowden, Judy, Barnes, Phillipa, Fitzharris, Penny, Craig, Jeffrey, Slykerman, Rebecca F., Crane, Julian“…Four hundred pregnant women expecting infants at high risk of allergic disease will be enrolled in the study at 14–16 weeks gestation and randomised to receive either Lactobacillus rhamnosus HN001 (6 × 10(9) colony-forming units per day (cfu/day)) or placebo until delivery and then continuing until 6 months post-partum, if breastfeeding. …”
Publicado 2016
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40092por Than, Nwe Ni, Tomlinson, Claire L., Haldar, Debashis, King, Andrew L., Moore, David, Newsome, Philip N.“…METHODS: All types of study design that investigate the effectiveness of cell therapies (haematopoietic, mesenchymal and unsorted cell types) of autologous or allogeneic origin and/or the use of granulocyte colony-stimulating factor in patients with CLD including ACLF will be included (except case reports). …”
Publicado 2016
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40093por Hellyer, Thomas P., Anderson, Niall H., Parker, Jennie, Dark, Paul, Van Den Broeck, Tina, Singh, Suveer, McMullan, Ronan, Agus, Ashley M., Emerson, Lydia M., Blackwood, Bronagh, Gossain, Savita, Walsh, Tim S., Perkins, Gavin D., Conway Morris, Andrew, McAuley, Daniel F., Simpson, A. John“…Patients with clinically suspected VAP undergo BAL, and VAP is confirmed by growth of a potential pathogen at > 10(4) colony-forming units per millilitre (CFU/ml). Patients are randomised 1:1, to either a ‘biomarker-guided recommendation on antibiotics’ in which BAL fluid is tested for IL-1β and IL-8 in addition to routine microbiology testing, or to ‘routine use of antibiotics’ in which BAL undergoes routine microbiology testing only. …”
Publicado 2016
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40094por Cheung, Chartia Ching-Mei, Lun, Samantha Wei-Man, Chung, Grace Tin-Yun, Chow, Chit, Lo, Carman, Choy, Kwong-Wai, Lo, Kwok-Wai“…Anchorage-dependent (colony formation) and anchorage-independent (tumor sphere formation) growths of these miR-96 and miR-183 expressing cells were determined. …”
Publicado 2016
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40095por Esparza-López, José, Ramos-Elías, Pier A., Castro-Sánchez, Andrea, Rocha-Zavaleta, Leticia, Escobar-Arriaga, Elizabeth, Zentella-Dehesa, Alejandro, León-Rodríguez, Eucario, Medina-Franco, Heriberto, Ibarra-Sánchez, María de Jesus“…Anchorage-independent cell growth was evaluated by colony formation in soft agar. RESULTS: Several subpopulations were isolated from the MBCDF breast cancer cells that were divided into two groups according to their morphology. …”
Publicado 2016
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40096por Qi, Defeng, Li, Jinhui, Que, Biao, Su, Jialin, Li, Mengxi, Zhang, Chaofeng, Yang, Mei, Zhou, Guoren, Ji, Weidong“…Cell proliferation assay, colony formation assay and flow cytometric analysis were employed to measure the role of DBCCR1-003 in regulation of cell proliferation, cycle and apoptosis. …”
Publicado 2016
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40097por Cepeda, Mario A., Pelling, Jacob J. H., Evered, Caitlin L., Williams, Karla C., Freedman, Zoey, Stan, Ioana, Willson, Jessica A., Leong, Hon S., Damjanovski, Sashko“…In 3D culture, MCF-7 breast cancer cells expressing low levels of MT1-MMP demonstrated an invasive protrusive phenotype, whereas cells expressing high levels of MT1-MMP demonstrated loss of colony structure and cell fragment release. Similarly, in vivo analysis demonstrated increased tumourigenecity and metastatic capability for cells expressing low levels of MT1-MMP, whereas cells expressing high levels were devoid of these qualities despite the production of functional MT1-MMP protein. …”
Publicado 2016
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40098por Huang, Teng, Yin, Li, Wu, Jing, Gu, Jia-Jia, Wu, Jian-Zhong, Chen, Dan, Yu, Hong-Liang, Ding, Kai, Zhang, Nan, Du, Ming-Yu, Qian, Lu-Xi, Lu, Zhi-Wei, He, Xia“…The effect of miR-19b-3p on radiosensitivity was evaluated by cell viability assays, colony formation assays and in vivo experiment. Apoptosis and cell cycle were examined by flow cytometry. …”
Publicado 2016
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40099por Estrago-Franco, M. Fernanda, Moustafa, M. Tarek, Riazi-Esfahani, Mohammad, Sapkal, Ashish U., Piche-Lopez, Rhina, Patil, A. Jayaprakash, Sharma, Ashish, Falatoonzadeh, Payam, Chwa, Marilyn, Luczy-Bachman, Georgia, Kuppermann, Baruch D., Kenney, M. Cristina“…Multiplex bead arrays were used to measure levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), Transforming Growth Factor alpha (TGF-α) and Vascular Endothelial Growth Factor (VEGF). …”
Publicado 2016
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40100“…Individual treatments with MSCs and Oh-LAAO in the infected model resulted in reduction of MRSA load by one order of magnitude to the approximate range of 6 log(10) colony-forming units (CFU) compared to untreated controls (7.3 log(10) CFU). …”
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