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40201por Zhu, Xiaojian, Bu, Fanqin, Tan, Ting, Luo, Qilin, Zhu, Jinfeng, Lin, Kang, Huang, Jun, Luo, Chen, Zhu, Zhengming“…The potential biological function of RP11-757G1.5 in CRC was investigated by Colony formation, Edu cell proliferation, Flow cytometry, Wound healing and Transwell assays. …”
Publicado 2020
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40202por Sun, Jiangang, Shen, Dandan, Zheng, Yichao, Ren, Hongmei, Liu, Hongmin, Chen, Xiaoping, Gao, Yongshun“…Initially, tumor samples and GC cell lines were used to detect USP8 and HER-2 levels. Next, MTT and colony formation assays were applied to analyze cell proliferation capability. …”
Publicado 2020
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40203“…Flow cytometry, transwell, wound-healing, CCK8 and colony formation assays were performed to analyse cell apoptosis, cell cycle, invasion, migration and proliferation. …”
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40204por Yu, Xiaozhen, Sun, Pengpeng, Huang, Xingang, Chen, Hua, Huang, Weiqing, Ruan, Yingchun, Jiang, Weina, Tan, Xiaohua, Liu, Zhijing“…Floating cells cultured in hematopoietic induction medium and stimulated with erythropoetic growth factors could transdifferentiate into mature erythrocytes, whereas adherent cells formed negligible hematopoietic colonies. The aim of this study was to reveal the role of cell morphology and adhesion on erythropoiesis induced by OCT4 in hHFMSCs and to characterize the molecular mechanisms involved. …”
Publicado 2020
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40205por Vadopalas, Laurynas, Ruzauskas, Modestas, Lele, Vita, Starkute, Vytaute, Zavistanaviciute, Paulina, Zokaityte, Egle, Bartkevics, Vadims, Pugajeva, Iveta, Reinolds, Ingars, Badaras, Sarunas, Klupsaite, Dovile, Mozuriene, Erika, Dauksiene, Agila, Gruzauskas, Romas, Bartkiene, Elena“…Compared to a commercially available lactic acid bacteria (LAB) combination, the novel LAB mixture effectively reduced feed pH (on average pH 3.65), produced a 2-fold higher content of L(+) lactic acid, increased viable LAB count [on average 8.8 log(10) colony-forming units (CFU) g(−1)], and led to stable feed fermentation during the entire test period (36 days). …”
Publicado 2020
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40206por Lian, Weibin, Jiang, Xiaohua, Li, Liangqiang, Wang, Qinglan, Hong, Chengye, Yang, Peidong, Chen, Debo“…Cell viability examination and colony formation experiments were performed to investigate the role of LL22NC03-N64E9.1 in BC cell’s proliferation. …”
Publicado 2020
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40207“…In the meantime, the gene expression level was detected in A2780, HO-8910PM, COC1, and SKOV3 cell lines (human ovarian carcinoma cell line). MTT and colony formation assays were used to detect cell proliferation of SKOV3 cell line. …”
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40208por Wang, Rui-Qi, Long, Xiao-Ran, Zhou, Ning-Ning, Chen, Dong-Ni, Zhang, Mei-Yin, Wen, Zhe-Sheng, Zhang, Lan-Jun, He, Fa-Zhong, Zhou, Zhi-Lin, Mai, Shi-Juan, Wang, Hui-Yun“…RESULTS: lnc-GAN1 is downregulated in HCC tissues and associated with larger tumor size and poor overall survival and disease-free survival; its ectopic expression suppresses cell proliferation, colony formation, and cell cycle progression and induces apoptosis in NSCLC cells; it also inhibits tumor growth in the NSCLC xenograft model. …”
Publicado 2021
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40209por Shi, Yu, Sun, Liankang, Zhang, Rui, Hu, Yuan, Wu, Yinying, Dong, Xuyuan, Dong, Danfeng, Chen, Chen, Geng, Zhimin, Li, Enxiao, Fan, Yangwei“…MTT viability assay, colony formation, EdU incorporation assay, flow cytometry analysis, Transwell assay, tumorsphere formation and western blot assays were performed to investigate the effects of CAF-derived TSP-4 on GBC cell proliferation, EMT and cancer stem-like features. …”
Publicado 2021
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40210por Zhao, Peng, Cheng, Jianhua, Li, Bin, Nie, Ding, Wang, Hongyun, Li, Chuzhong, Gui, Songbai, Zhang, Yazhuo“…The viability, apoptosis, cell cycle distribution of PA cells, as well as their ability to invade, migrate, and proliferate, were tested after transfection through CCK-8, flow cytometry, transwell, wound healing, and colony formation assays. After construction of transplanted-tumor model in nude mice, cell apoptosis in the tumor was detected by TUNEL. …”
Publicado 2021
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40211por Hyroššová, Petra, Aragó, Marc, Moreno-Felici, Juan, Fu, Xiarong, Mendez-Lucas, Andrés, García-Rovés, Pablo M., Burgess, Shawn, Figueras, Agnès, Viñals, Francesc, Perales, Jose C.“…Finally, PEPCK-M loss negatively impacted on anchorage-independent colony formation and xenograft growth in vivo. CONCLUSIONS: All in all, our data suggest that PEPCK-M might participate in the mechanisms to regulate proteostasis in the anabolic and stalling phases of tumor growth. …”
Publicado 2021
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40212por Zhao, Zhidong, Wang, Yuxing, Wang, Qian, Liang, Jiawu, Hu, Wei, Zhao, Sen, Li, Peilin, Zhu, Heng, Li, Zhongli“…The optimized parameters for SCB-SPC self-renewal were screened out by colony-forming unit fibroblast assay (CFU-F). Then, the effects of r-ESW on the proliferation, apoptosis, and multipotency of SCB-SPCs were evaluated. …”
Publicado 2021
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40213por Zhang, Xing, Yang, Hongwei, Jia, Yingdong, Xu, Zhengwen, Zhang, Liuping, Sun, Meng, Fu, Jing“…Cell proliferation and cell migration were determined by MTT, EdU incorporation, colony formation, wound scratch and transwell assays. …”
Publicado 2021
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40214por Brownlie, Demi, Doughty-Shenton, Dahlia, YH Soong, Daniel, Nixon, Colin, O Carragher, Neil, M Carlin, Leo, Kitamura, Takanori“…The effects of MAM depletion on NK cell activation, maturation, and accumulation in the metastatic lung were evaluated by flow cytometry (CD69, CD11b, CD27) and in situ hybridization (Ncr1) using colony-stimulating factor 1 (CSF-1) receptor conditional knockout (Csf1r-cKO) mice. …”
Publicado 2021
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40215por Jiang, Weidong, Zhu, Peiqi, Zhang, Tao, Liao, Fengchun, Yu, Yangyang, Liu, Yan, Shen, Huijuan, Zhao, Zhenchen, Huang, Xuanping, Zhou, Nuo“…Peripheral blood samples were collected to isolate serum and endothelial colony-forming cells (ECFCs) in order to measure miR-205, NOTCH2, and angiogenic cytokines expression levels. …”
Publicado 2021
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40216“…Cell cloning ability and apoptosis were examined by colony formation and flow cytometry assays, respectively. …”
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40217por Tan, Shuo, Kang, Ye, Li, Hu, He, Hai-Qing, Zheng, Long, Wu, Shui-Qing, Ai, Kai, Zhang, Lei, Xu, Ran, Zhang, Xuan-Zhi, Zhao, Xiao-Kun, Zhu, Xuan“…In this study, we first screened circRNA expression profiles using a circRNA microarray of paired BCa and normal tissues, and the expression of circST6GALNAC6 was confirmed by qRT-PCR and fluorescence in situ hybridization (FISH). MTT, colony formation and Transwell assays were performed to measure cell proliferation, migration and invasion. …”
Publicado 2021
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40218“…We performed cell function experiments (CCK-8 assay, EdU assay, colony formation assay, wound healing assay, transwell assay, cell apoptosis assessment, and cell cycle assessment) and nude mouse tumor formation experiments to evaluate the effects of miR-378a-5p on proliferation, metastasis, and invasion to explore the role of miR-378a-5p in vivo and in vitro. …”
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40219por Abdelgawad, Mohamed Essameldin, Desterke, Christophe, Uzan, Georges, Naserian, Sina“…This shortened database of EPC factors was fed into publically published transcriptome matrix to compare their expression between endothelial colony-forming cells (ECFCs), HUVECs, and two adult endothelial cell types (ECs) from the skin and adipose tissue. …”
Publicado 2021
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40220“…METHODS: Cell counting kit-8 (CCK-8) assay and colony formation assay were performed to evaluate cell proliferation abilities. …”
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