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40621por Ashuro, Zemachu, Aregu, Mekonnen Birhanie, Kanno, Girum Gebremeskel, Negassa, Belay, Soboksa, Negasa Eshete, Alembo, Awash, Ararsa, Eshetu, Badecha, Fikru, Tassew, Solomon“…After incubation of 14 hours at 44°C, faecal coliforms with yellow colonies were counted and expressed in terms of CFU/100 ml of water. …”
Publicado 2021
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40622por Fernandez-Novo, Aitor, Santos-Lopez, Sergio, Barrajon-Masa, Clara, Mozas, Patricia, de Mercado, Eduardo, Caceres, Elisa, Garrafa, Aizic, Gonzalez-Martin, Juan Vicente, Perez-Villalobos, Natividad, Oliet, Agustin, Astiz, Susana, Perez-Garnelo, Sonia Salome“…Acrosome integrity, sperm viability and morphology, CASA-total and progressive motility, pH, and colony-forming units were assessed. Semen quality was preserved for up to 4–6 h post-ejaculation, except for INRA96(®) at 5 °C. …”
Publicado 2021
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40623por Collin, Joseph, Queen, Rachel, Zerti, Darin, Bojic, Sanja, Dorgau, Birthe, Moyse, Nicky, Molina, Marina Moya, Yang, Chunbo, Dey, Sunanda, Reynolds, Gary, Hussain, Rafiqul, Coxhead, Jonathan M., Lisgo, Steven, Henderson, Deborah, Joseph, Agatha, Rooney, Paul, Ghosh, Saurabh, Clarke, Lucy, Connon, Che, Haniffa, Muzlifah, Figueiredo, Francisco, Armstrong, Lyle, Lako, Majlinda“…RNA interference, IF, colony forming efficiency and clonal assays were performed on cultured limbal epithelial cells (LECs). …”
Publicado 2021
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40624por Chen, Junyu, Liu, Jian, Wu, Shan, Liu, Wei, Xia, Yang, Zhao, Jing, Yang, Yanrong, Wang, Yuan, Peng, Yuanqing, Zhao, Shuhua“…After exposure to ATZ, the MTT assay, flow cytometry, the colony formation assay, immunohistochemical staining, the cell scratch assay, and the Transwell assay were used to evaluate the proliferative activity, invasion, and migration capabilities of EOC cell lines. …”
Publicado 2021
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40625por Ludden, Catherine, Coll, Francesc, Gouliouris, Theodore, Restif, Olivier, Blane, Beth, Blackwell, Grace A, Kumar, Narender, Naydenova, Plamena, Crawley, Charles, Brown, Nicholas M, Parkhill, Julian, Peacock, Sharon J“…Our findings challenge single colony-based investigations, and the widely accepted notion of plasmid spread. …”
Publicado 2021
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40626por Scieuzo, Carmen, Nardiello, Marisa, Farina, Donatella, Scala, Andrea, Cammack, Jonathan A., Tomberlin, Jeffery K., Vogel, Heiko, Salvia, Rosanna, Persaud, Krishna, Falabella, Patrizia“…Mass production of this insect in colonies requires the development of methods concentrating oviposition in specific collection devices, while the mass production of larvae and disposing of waste may require substrates that are more palatable and more attractive to the insects. …”
Publicado 2021
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40627por Syed, Asad, Zeyad, Mohammad Tarique, Shahid, Mohammad, Elgorban, Abdallah M., Alkhulaifi, Manal M., Ansari, Irfan Aamer“…While assessing the toxic effect of HMs on the P-solubilizing activity of E. cloacae, the toxicity pattern followed the order Cr (mean value = 94.6 μg mL(–1)) > Cd (mean value = 127.2 μg mL(–1)) > Pb (mean value = 132.4 μg mL(–1)) > Ni (mean value = 140.4 μg mL(–1)). Furthermore, the colony-forming unit (CFU) count (Log(10)) of strain MC9 was completely inhibited at 150, 175, and 200 μg mL(–1) concentrations of Cr and Cd. …”
Publicado 2021
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40628por Li, Qingrong, Ma, Qiang, Xu, Lei, Gao, Chuanli, Yao, Lihua, Wen, Jilin, Yang, Miyuan, Cheng, Jibing, Zhou, Xi, Zou, Jiang, Zhong, Xiaowu, Guo, Xiaolan“…The Cell Counting Kit-8 (CCK8) and colony formation assay were used to evaluate the proliferation of esophageal squamous cancer cell lines (Eca109, KYSE150, and TE1) after hTERT overexpression or treated with indicated concentrations of DHA. …”
Publicado 2021
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40629por Vainchenker, William, Arkoun, Brahim, Basso-Valentina, Francesca, Lordier, Larissa, Debili, Najet, Raslova, Hana“…Abbreviations: AKT: Protein Kinase BARHGEF2: Rho/Rac Guanine Nucleotide Exchange Factor 2ARP2/3: Actin related protein 2/3BM: Bone marrowCDC42: Cell division control protein 42 homologCFU-MK: Colony-forming-unit megakaryocyteCIP4: Cdc42-interacting protein 4mDIA: DiaphanousDIAPH1; Protein diaphanous homolog 1ECT2: Epithelial Cell Transforming Sequence 2FLNA: Filamin AGAP: GTPase-activating proteins or GTPase-accelerating proteinsGDI: GDP Dissociation InhibitorGEF: Guanine nucleotide exchange factorHDAC: Histone deacetylaseLIMK: LIM KinaseMAL: Megakaryoblastic leukaemiaMARCKS: Myristoylated alanine-rich C-kinase substrateMKL: Megakaryoblastic leukaemiaMLC: Myosin light chainMRTF: Myocardin Related Transcription FactorOTT: One-Twenty Two ProteinPACSIN2: Protein Kinase C And Casein Kinase Substrate In Neurons 2PAK: P21-Activated KinasePDK: Pyruvate Dehydrogenase kinasePI3K: Phosphoinositide 3-kinasePKC: Protein kinase CPTPRJ: Protein tyrosine phosphatase receptor type JRAC: Ras-related C3 botulinum toxin substrate 1RBM15: RNA Binding Motif Protein 15RHO: Ras homologousROCK: Rho-associated protein kinaseSCAR: Suppressor of cAMP receptorSRF: Serum response factorSRC: SarcTAZ: Transcriptional coactivator with PDZ motifTUBB1: Tubulin β1VEGF: Vascular endothelial growth factorWAS: Wiskott Aldrich syndromeWASP: Wiskott Aldrich syndrome proteinWAVE: WASP-family verprolin-homologous proteinWIP: WASP-interacting proteinYAP: Yes-associated protein…”
Publicado 2021
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40630“…All positive cultures were characterized by colony morphology, Gram stain, and standard biochemical tests. …”
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40631por Ke, Yao, Chen, Xiaoying, Su, Yuting, Chen, Cuilan, Lei, Shunmei, Xia, Lianping, Wei, Dan, Zhang, Han, Dong, Caihua, Liu, Xia, Yin, Fuqiang“…In vitro overexpression of SLC7A11 significantly increased the sensitivity of HeyA8-R cells to paclitaxel, inhibited colony formation, induced apoptosis, and arrested cell cycle. …”
Publicado 2021
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40632por Wanford, Joseph J, Hames, Ryan G, Carreno, David, Jasiunaite, Zydrune, Chung, Wen Y, Arena, Fabio, Di Pilato, Vincenzo, Straatman, Kornelis, West, Kevin, Farzand, Robeena, Pizza, Mariagrazia, Martinez-Pomares, Luisa, Andrew, Peter W, Moxon, E Richard, Dennison, Ashley R, Rossolini, Gian Maria, Oggioni, Marco R“…METHODS: In this exploratory investigation, to study the pathophysiology of abscess formation, mice were intravenously infected with 10(6) colony forming units (CFU) of either hvKp isolates (six strains) or non-hvKp isolates (seven strains). …”
Publicado 2021
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40633por Gou, Yaping, Jin, Wei, He, Yanning, Luo, Yu, Si, Ruirui, He, Yuan, Wang, Zhongchi, Li, Jing, Liu, Bin“…For antibacterial testing, Streptococcus mutans, Actinomyces naeslundii, and Enterococcus faecalis were grown on dentin surfaces, prior to the application of cavity cleanser. Colony-forming unit (CFU) counts and live/dead bacterial staining were performed to assess antibacterial effects. …”
Publicado 2021
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40634“…METHODS: AC and PC were evaluated, in duplicate, for synergy utilizing 24-hour in vitro time-kill assays with a starting inoculum of 10(6) colony forming units (CFU)/mL. Six clinical E. faecalis blood isolates and one wild-type E. faecalis isolate (JH2-2) were included. …”
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40635por Baxter, Diana E., Allinson, Lisa M., Al Amri, Waleed S., Poulter, James A., Pramanik, Arindam, Thorne, James L., Verghese, Eldo T., Hughes, Thomas A.“…MicroRNA/mRNA expression was manipulated in estrogen receptor positive/HER2 negative breast cancer cell lines (MCF7 and MDA-MB-175 cells) with mimics, inhibitors or siRNAs, and chemoresponse was assessed using MTT and colony forming survival assays. MicroRNA targets were identified by RNA-sequencing of microRNA mimic pull-downs, and comparison of these with mRNAs containing predicted microRNA binding sites. …”
Publicado 2021
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40636por Mi, Yuan-yuan, Sun, Chuan-yu, Zhang, Li-feng, Wang, Jun, Shao, Hong-bao, Qin, Feng, Xia, Guo-wei, Zhu, Li-jie“…Additionally, we built a lncRNA–miRNA–mRNA ceRNA network through bioinformatics analysis. Colony formation, CCK-8, flow cytometry, scratch, and Transwell assays were performed based on PCa cells subjected to small interfering RNA (siRNA) targeting LINC01679/SLC17A9 and vector expressing LINC01679/SLC17A9 transfection. …”
Publicado 2021
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40637por Liang, Shanshan, Huang, Guixian, Wu, Tian, Peng, Ying, Liu, Xi, Ji, Xuejiao, Sha, Wei, Wang, Feifei, Shen, Ling, Shen, Hongbo“…Furthermore, a higher expression of Mir337-3p after mycobacterial infection of mice coincided with much greater colony-forming unit (CFU) counts in lungs and even the death of infected animals, whereas Mir337-3p inhibitor treatment of infected mice reduced Mir337-3p levels and reversed Mir337-3p-mediated increases in CFU counts. …”
Publicado 2021
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40638por Altaie, Ala, Baboolal, Thomas G., Wall, Owen, Pandit, Hemant, Jones, Elena, McGonagle, Dennis“…Human knee synovium (n = 5) was collected during total knee replacement, and a suspended culture was performed to assess the effect of the STEM device on ongoing MSC release. Colony forming unit–fibroblastic assays were used to determine the number of MSCs. …”
Publicado 2021
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40639por Liu, Donghong, Liu, Wenbin, Chen, Xi, Yin, Jianhua, Ma, Longteng, Liu, Mei, Zhou, Xinyu, Xian, Linfeng, Li, Peng, Tan, Xiaojie, Zhao, Jun, Liao, Yong, Cao, Guangwen“…Ectopic expression of circKCNN2 inhibited HCC cell proliferation, colony formation, migration, and tumor formation in a mouse model. miR‐520c‐3p sponged by circKCNN2 could reverse the inhibitory effect of circKCNN2 on HCC cells and down‐regulate the expression of methyl‐DNA‐binding domain protein 2 (MBD2). …”
Publicado 2022
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40640por Takemegni, Wandji Jonas Merlin, Assob, Nguedia Jules Clement, Ateudjeu, Jeurome, Enow, Orock George, Ngowe, Ngowe Marcelin“…Identification and confirmation were based on morphological characteristics of bacteria colonies, microscopy and biochemical methods using API staph, strepto and 20ETM gallery (bioMérieux). …”
Publicado 2022
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