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40641“…Cell counting kit-8 assay, 5-bromo-2′-deoxyuridine proliferation assay, and colony-formation assay were performed to evaluate cell viability and proliferation. …”
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40642por Olivarria, Gema M., Cheng, Yuting, Furman, Susana, Pachow, Collin, Hohsfield, Lindsay A., Smith-Geater, Charlene, Miramontes, Ricardo, Wu, Jie, Burns, Mara S., Tsourmas, Kate I., Stocksdale, Jennifer, Manlapaz, Cynthia, Yong, William H., Teijaro, John, Edwards, Robert, Green, Kim N., Thompson, Leslie M., Lane, Thomas E.“…Microglia depletion via administration of colony-stimulating factor 1 receptor inhibitor, PLX5622, in SARS-CoV-2 infected mice did not affect survival or viral replication but did result in dampened expression of proinflammatory cytokine/chemokine transcripts and a reduction in monocyte/macrophage infiltration. …”
Publicado 2022
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40643por Liikanen, Ilkka, Basnet, Saru, Quixabeira, Dafne C A, Taipale, Kristian, Hemminki, Otto, Oksanen, Minna, Kankainen, Matti, Juhila, Juuso, Kanerva, Anna, Joensuu, Timo, Tähtinen, Siri, Hemminki, Akseli“…METHODS: Modulation of TIM-3 expression on tumor-infiltrating immune cells was studied preclinically in B16 melanoma following intratumoral treatment with Ad5/3∆24-granulocyte-macrophage colony-stimulating factor oncolytic adenovirus. We conducted a retrospective longitudinal analysis of 15 patients with advanced-stage cancer with tumor-site biopsies before and after oncolytic immunotherapy, treated in the Advanced Therapy Access Program (ISRCTN10141600, April 5, 2011). …”
Publicado 2022
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40644por Pimentel, Bruna Natália Alves da Silva, Marin-Dett, Freddy Humberto, Assis, Marcelo, Barbugli, Paula Aboud, Longo, Elson, Vergani, Carlos Eduardo“…The action of microcrystals on C. albicans biofilm was evaluated by colony-forming units (CFU/ml) and LIVE/DEAD staining. …”
Publicado 2022
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40645por Tan, Xingliang, Yuan, Gangjun, Wang, Yanjun, Zou, Yuantao, Luo, Sihao, Han, Hui, Qin, Zike, Liu, Zhuowei, Zhou, Fangjian, Liu, Yanling, Yao, Kai“…Furthermore, tumorigenesis assays demonstrated that RAB20 knockdown inhibited cell proliferation, migration, and colony formation in vitro and tumor growth in vivo. …”
Publicado 2022
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40646“…Quantitative and qualitative counts (aerobic colony county [ACC], adenosine triphosphate [ATP]), hydrogen peroxide chemical indicators (CIs) and bacterial spore biological indicators (G. stearothermophilus 1x10(6) spores) were measured. …”
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40647“…He received two doses of granulocyte colony stimulating factor (G-CSF). The subsequent ANC two days after the second dose was 3,600/uL. …”
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40648por Machani, Maxwell G., Ochomo, Eric, Amimo, Fred, Mukabana, Wolfgang R., Githeko, Andrew K., Yan, Guiyun, Afrane, Yaw A.“…METHODS: Susceptible and pyrethroid-resistant colonies of female Anopheles gambiae mosquitoes aged 3–5 days old were used in this study. …”
Publicado 2022
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40649TERT Expression in Wilms Tumor Is Regulated by Promoter Mutation or Hypermethylation, WT1, and N-MYCpor Jablonowski, Carolyn M., Gil, Hyea Jin, Pinto, Emilia M., Pichavaram, Prahalathan, Fleming, Andrew M., Clay, Michael R., Hu, Dongli, Morton, Christopher L., Pruett-Miller, Shondra M., Hansen, Baranda S., Chen, Xiang, Jones, Karissa M. Dieseldorff, Liu, Yanling, Ma, Xiaotu, Yang, Jun, Davidoff, Andrew M., Zambetti, Gerard P., Murphy, Andrew J.“…In vitro shRNA knockdown of WT1 resulted in decreased expression of TERT, reduced colony formation, and decreased proliferation of WiT49, an anaplastic WT cell line with wild-type WT1. …”
Publicado 2022
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40650por Liu, Li, He, Jiangtu, Sun, Guifeng, Huang, Nan, Bian, Zhixuan, Xu, Chang, Zhang, Yue, Cui, Zhongqi, Xu, Wenqiang, Sun, Fenyong, Zhuang, Chengle, Man, Qiuhong, Gu, Song“…METHODS: Reverse transcription quantitative real‐time PCR (RT‐qPCR) and western blotting were used to measure SLC7A11 levels. Cell proliferation, colony formation, lipid reactive oxygen species (ROS), MDA concentration, 4‐HNE, GSH/GSSG ratio and cell death assays as well as subcutaneous xenograft experiments were used to elucidate the effects of SLC7A11 in HB cell proliferation and ferroptosis. …”
Publicado 2022
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40651por Mascarin, Gabriel Moura, da Silva, Ana Vitória Reina, da Silva, Thiago Pereira, Kobori, Nilce Naomi, Morandi, Marcelo Augusto Boechat, Bettiol, Wagner“…The fermentation batches carried out in a benchtop bioreactor with medium 50:1 C:N ratio and amended with soybean meal rendered a production peak on the fourth day, corresponding to 1.11 × 10(9) conidia/ml and 4.35 × 10(8) colony forming units (CFU)/ml. Following air-drying, the conidia production from air-dried microgranules of C. rosea biomass was estimated at 3.4 × 10(10) conidia/g of formulated product upon re-hydration for 7 days. …”
Publicado 2022
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40652por Viola, Nerissa T., Glassbrook, James E., Kalluri, Jhansi R., Hackett, Justin B., Wicker, Madison N., Sternberg, Joshua, Gibson, Heather M.“…To evaluate the utility of [(89)Zr]Zr-DFO-αIL12 as an indicator of antigen presenting cell activation after cancer immunotherapy, we compared PET imaging with and without intratumoral delivery of oncolytic adenovirus expressing granulocyte-macrophage colony-stimulating factor (Adv/GM-CSF), which we have shown promotes anti-tumor immunity. …”
Publicado 2022
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40653“…After in vitro digestion, ten LAB colonies were randomly isolated. Poi from three different batches were tested independently. …”
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40654por Kiambi, Stella, Fèvre, Eric M., Alarcon, Pablo, Gitahi, Nduhiu, Masinde, Johnstone, Kang'ethe, Erastus, Aboge, Gabriel, Rushton, Jonathan, Onono, Joshua Orungo“…Cow milk products (N = 290) were collected from farms (N = 63), collection centers (N = 5), shops/kiosks (N = 37), milk bars (N = 17), roadside vendors (N = 14), restaurants (N = 3), milk vending machines (N = 2), mobile traders (N = 2) and a supermarket (N = 1). Mean values of colony-forming units for TBC and TCC were referenced to East African Standards (EAS). …”
Publicado 2022
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40655por Badiee, Parisa, Boekhout, Teun, Zarei Mahmoudabadi, Ali, Mohammadi, Rasoul, Ayatollahi Mousavi, Seyyed Amin, Najafzadeh, Mohammad Javad, Soltani, Jafar, Hashemi, Jamal, Diba, Kambiz, Ghadimi-Moghadam, Abdolkarim, Salimi-Khorashad, Ali Reza, Shokohi, Tahereh, Amin Shahidi, Maneli, Ghasemi, Fatemeh, Jafarian, Hadis“…Aspergillus isolates were identified based on colony morphology, microscopic characteristics, PCR-restriction fragment length polymorphism (RFLP), and sequencing of the beta-tubulin gene. …”
Publicado 2022
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40656“…Cell proliferation ability was detected by CCK-8, EdU, colony formation assay and xenograft tumor in nude mice. …”
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40657por Xu, Kequan, Xia, Peng, Gongye, Xiangdong, Zhang, Xiao, Ma, Shuxian, Chen, Zhang, Zhang, Hao, Liu, Jie, Liu, Yingyi, Guo, Yonghua, Yao, Ye, Gao, Meng, Chen, Yiran, Zhang, Zhonglin, Yuan, Yufeng“…METHODS: Through analysis and screening of The Cancer Genome Atlas-Liver Hepatocellular Carcinoma (TCGA-LIHC) dataset, we found a novel lncRNA RP11-386G11.10 was overexpressed, related to prognosis, conserved and non-protein-coding in HCC and related to poor prognosis. Then, CCK-8, colony formation, Transwell invasion, wound healing assays were performed and nude mouse subcutaneous tumour formation and lung metastasis models were established to explore the effect of RP11-386G11.10 on HCC tumour growth and metastasis. …”
Publicado 2022
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40658por Knapp, Ingrid S.S., Forsman, Zac H., Greene, Austin, Johnston, Erika C., Bardin, Claire E., Chan, Norton, Wolke, Chelsea, Gulko, David, Toonen, Robert J.“…However, the major causes of variation in growth and survival of outplanted colonies remain poorly understood. Here, we report simple outplanting assays to aid in active reef restoration of slower growing species and increase the likelihood of restoration success. …”
Publicado 2022
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40659por Essex, Alyson L., Huot, Joshua R., Deosthale, Padmini, Wagner, Alison, Figueras, Jorge, Davis, Azaria, Damrath, John, Pin, Fabrizio, Wallace, Joseph, Bonetto, Andrea, Plotkin, Lilian I.“…Only male TREM2(R47H/+) osteoclasts differentiated more ex vivo after 7 days with receptor activator of nuclear factor κB ligand (RANKL)/macrophage colony‐stimulating factor (M‐CSF) compared to WT littermates. …”
Publicado 2022
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40660por Wang, Xiaoyan, Wang, Yiwen, Sun, Fusheng, Xu, Yang, Zhang, Zhaocong, Yang, Chang, Zhang, Lijie, Lou, Ge“…The effect of lncRNA ZFHX4-AS1 on proliferation, invasion and migration of OvCa cells was verified through Cell Counting Kit (CCK)-8, colony formation, wound healing and transwell assays. …”
Publicado 2022
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