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2001“…by establishing a core outcome set. TRIAL REGISTRATION: COMET initiative study, 902. Registered in May 2016. …”
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2002por Crudgington, Holly, Rogers, Morwenna, Bray, Lucy, Carter, Bernie, Currier, Janet, Dunkley, Colin, Gibbon, Frances M., Hughes, Dyfrig, Lyle, Samantha, Roberts, Deborah, Tudur Smith, Catrin, Gringras, Paul, Pal, Deb K., Morris, Christopher“…METHODS: We followed guidance from the COMET (Core Outcome Measures in Effectiveness Trials) Initiative. …”
Publicado 2019
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2003por Piña Olmos, Sofia, Díaz Torres, Roberto, Elbakrawy, Eman, Hughes, Louise, Mckenna, Joseph, Hill, Mark A., Kadhim, Munira, Ramírez Noguera, Patricia, Bolanos-Garcia, Victor M.“…Our measurements of metabolic activity as an indicator of cell viability, DNA damage by alkaline comet assay, and immunofluorescence using anti-P-H3 as a mitotic biomarker indicate that chitosan nanoparticles elicit cellular responses that affect mitosis and cell viability and can sensitise breast cancer cells to X-ray radiation (2Gy). …”
Publicado 2019
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2004por Von Trompowsky, Ana Claudia Manoel, Conde, Taline Ramos, Lemos, Renata Calil, Quaresma, Bruna Maria CS, Pitombeira, Marcelly Cristina SR, de Carvalho, Alcione Silva, Boechat, Núbia, Salomão, Kelly, de Castro, Solange Lisboa, Zamith, Helena Pereira da Silva“…OBJECTIVES: To evaluate whether the genotoxic effect of 3 was abolished in the seven nitroimidazoles (4-10) analogs using the in vitro alkaline comet assay (CA) and the in vitro cytokinesis-block micronucleus assay (CBMN) in whole human blood cells (WHBC) and correlate this effect with their trypanocidal activity using bloodstream trypomastigote forms of Trypanosoma cruzi. …”
Publicado 2019
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2005por Araújo, Susana, Pagano, Andrea, Dondi, Daniele, Lazzaroni, Simone, Pinela, Eduardo, Macovei, Anca, Balestrazzi, Alma“…Results from alkaline comet assay, highlighting the occurrence of DNA damage at this stage of germination, are consistent with the hypothesis that prolonged exposure to kinetin induces stress conditions leading to genotoxic injury.…”
Publicado 2019
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2006“…Then, the biological effects of MF pre-exposed culture medium on cell viability, the mitogen-activated protein kinase (MAPK) signaling pathways, oxidative stress, and genetic stabilities were analyzed using Cell Counting Kit-8, western blot, flow cytometry, γH2AX foci formation, and comet assay. The results showed that SPR signals were decreased under MF exposure in a time- and dose-dependent manner, and the decreased SPR signals were reversible when the exposure was drawn off. …”
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2007por Zhang, Fang, Ren, Liang, Zhou, Shanshan, Duan, Peng, Xue, Junchao, Chen, Haiqin, Feng, Yufeng, Yue, Xiaoxuan, Yuan, Piaofan, Liu, Qizhan, Yang, Ping, Lei, Yixiong“…We used Trypan blue exclusion assay for cell growth, MTT colorimetric assays for cells inhibition rate, and Comet assays for detecting damaged DNA. RESULTS: CdCl(2), at various concentrations and exposure times, increased BOK mRNA. 16HBE-BOK cells (BOK over-expressing) proliferated more than 16HBE cells after 72 h; 16HBE-shBOK (BOK knockdown) cells proliferated less. …”
Publicado 2019
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2008por YAMAMOTO, Naoki, SHOJI, Momoka, HOSHIGAMI, Hiroki, WATANABE, Kohei, WATANABE, Kohei, TAKATSUZU, Tappei, YASUDA, Shin, IGOSHI, Keiji, KINOSHITA, Hideki“…These strains were selected, and a comet assay was performed, which exhibited decreased values in all selected strains compared with the control, indicating DNA protection. …”
Publicado 2019
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2009por Lorenzo, Yolanda, Haug Berg, Kristiane, Ringvold, Amund, Petrovski, Goran, Moe, Morten C., Collins, Andrew, Nicolaissen, Bjørn“…Cells were analysed after two, three and four weeks in culture for DNA strand breaks and oxidized purine bases (Comet assay using the enzyme formamidopyrimidine DNA glycosylase, Fpg) and for expression of DNA repair enzymes APE1, OGG1 and Polβ by in situ hybridization (ISH) and by immunohistochemistry (IHC). …”
Publicado 2018
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2010por Chen, Jiali, Gong, Fang, Chen, Mei-Fang, Li, Chengyong, Hong, Pengzhi, Sun, Shengli, Zhou, Chunxia, Qian, Zhong-Ji“…Furthermore, LSGYGP significantly decreased Ang II-induced DNA damage in a comet assay, and molecular docking results showed that the steady interaction between LSGYGP with NF-κB may be attributed to hydrogen bonds. …”
Publicado 2019
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2011por Que, Zujun, Zhou, Zhiyi, Luo, Bin, Dong, Changsheng, Jiang, Yi, Li, Hegen, Tian, Jianhui“…Flow cytometry was used to detect the apoptosis and the expression level of ROS and Caspase-3. Comet and TUNEL assays were used to detect DNA damage. …”
Publicado 2019
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2012por Giallourou, Natasa S., Rowland, Ian R., Rothwell, Steve D., Packham, Graham, Commane, Daniel M., Swann, Jonathan R.“…METHODS: (1)H nuclear magnetic resonance spectroscopy-based metabolic profiling was coupled with DNA damage-related assays (cell cycle, Comet assay, viability assays) to profile the comparative effects of watercress and PEITC in MCF-7 breast cancer cells and MCF-10A non-tumorigenic breast cells with and without exposure to IR. …”
Publicado 2018
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2013por Kedhari Sundaram, Madhumitha, Raina, Ritu, Afroze, Nazia, Bajbouj, Khuloud, Hamad, Mawieh, Haque, Shafiul, Hussain, Arif“…In the present study, we have examined the anti-ancer property of ubiquitous phytochemical quercetin by using cell viability assay, flow cytometry, nuclear morphology, colony formation, scratch wound assay, DNA fragmentation and comet assay. Further, qPCR analysis of various genes involved in apoptosis, cell cycle regulation, metastasis and different signal transduction pathways was performed. …”
Publicado 2019
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2014“…Co-IP was performed to evaluate the interaction between β-arrestin1 and ATR or H2AX. Comet assay was used to detect DNA damage. β-arrestin1 mRNA level co-related with ATR and H2AX levels in cancer tissues, and β-arrestin1 bound to ATR and H2AX directly or indirectly. …”
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2015por Coelho, Fernanda, Cavicchioli, Maurício, Specian, Sybele Saska, Scarel-Caminaga, Raquel Mantuaneli, Penteado, Letícia de Aquino, de Medeiros, Alexandra Ivo, Ribeiro, Sidney José de Lima, Capote, Ticiana Sidorenko de Oliveira“…No toxic potential was found in MC3T3-E1 cells by cytotoxicity assays (XTT Assay and Clonogenic Survival), genotoxicity (Comet Assay) and mutagenicity (Cytokinesis-blocked micronucleus Test). …”
Publicado 2019
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2016por Hua, Xiangwei, Sanjiv, Kumar, Gad, Helge, Pham, Therese, Gokturk, Camilla, Rasti, Azita, Zhao, Zhenjun, He, Kang, Feng, Mingxuan, Zang, Yunjin, Zhang, Jianjun, Xia, Qiang, Helleday, Thomas, Warpman Berglund, Ulrika“…HCC cancer cell lines were exposed in vitro to MTH1 inhibitors or depleted of MTH1 by siRNA. 8-oxoG was measured by the modified comet assay. The effect of MTH1 inhibition on tumor growth was explored in HCC xenograft in vivo models. …”
Publicado 2019
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2017por Mustyatsa, V. V., Kostarev, A. V., Tvorogova, A. V., Ataullakhanov, F. I., Gudimchuk, N. B., Vorobjev, I. A.“…Those secondary peaks, which we refer to as EB3-islands, were detected on 56% comets of growing MTs and were encountered once per 44 s of EB3-RFP comet growth time with about 5 s half-lifetime. …”
Publicado 2019
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2018“…The presence of DNA lesions was assessed by measurement phosphorylation of H2AX and by using the alkaline comet assay with proteinase K post-treatment following assessment of the cell cycle. …”
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2019por Saffari_Chaleshtori, Javad, Heidari-Sureshjani, Ehsan, Moradi, Fahimeh, Heidarian, Esfandiar“…Apoptotic and cytotoxicity effects of TQ were analyzed using flowcytometry and comet assay, respectively. Changes in energy and the molecular interactions of TQ with BCL-XL, BCL-2 and MCL-1 anti-apoptotic factors were investigated using simulation. …”
Publicado 2019
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2020por Sutris, JM, How, V, Sumeri, SA, Muhammad, M, Sardi, D, Mohd Mokhtar, MT, Mohammad, H, Ghazi, HF, Isa, ZM“…For genotoxic risk or genetic damage assessment, the level of DNA damage from exfoliated buccal mucosa cells was measured using the comet assay electrophoresis method. RESULTS: Out of 180 respondents, 84 (46.7%) showed positive traces of organophosphate metabolites in their urine. …”
Publicado 2016
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