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2181por Brevik, Asgeir, Gaivão, Isabel, Medin, Tirill, Jørgenesen, Aud, Piasek, Anita, Elilasson, Johanna, Karlsen, Anette, Blomhoff, Rune, Veggan, Turid, Duttaroy, Asim K, Collins, Andrew R“…Effects on DNA damage in circulating lymphocytes were estimated using the comet assay with enzyme modification to measure specific lesions; another modification allowed estimation of DNA repair. …”
Publicado 2011
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2182por Khuda-Bukhsh, Anisur Rahman, Bhattacharyya, Soumya Sundar, Paul, Saili, Dutta, Suman, Boujedaini, Naoual, Belon, Philippe“…There was reduction in genotoxic and DNA damages in bone marrow cells of Sec Cor 30-fed mice, as revealed from cytogenetic and Comet assays. Changes in histological features of skin papilloma were noted. …”
Publicado 2011
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2183por Zhang, Ming, He, Zuping, Wen, Lixin, Wu, Jing, Yuan, Liyun, Lu, Yin, Guo, Chengzhi, Zhu, Li, Deng, Sijun, Yuan, Hui“…Inhibitor SB202190 alleviated the proliferation inhibition of cadmium on piglet Sertoli cells. Comet assay revealed that cadmium chloride caused DNA damage of Piglet Sertoli cells and resulted in cell apoptosis as assayed by flow cytometry. …”
Publicado 2010
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2184“…During the process of migration of the GC to the VC, multiple and thick bundles of MTs, radiating from the cytoplasm near GC plasma membrane, arranged perpendicular to the narrow end of the GC and organized into a “comet-tail” form. These GC “tail” MTs became shortened and the generative nucleus (GN) took a ball shape. …”
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2185por De Stefano, Daniela, Tommonaro, Giuseppina, Malik, Shoaib Ahmad, Iodice, Carmine, De Rosa, Salvatore, Maiuri, Maria Chiara, Carnuccio, Rosa“…Results were obtained by using DNA fragmentation, comet and viability assays, quantification of the mitochondrial transmembrane potential and Western blot. …”
Publicado 2012
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2186“…Consistent with early drug accumulation and intimate interactions with the blood supply, stroma cell-enriched populations exhibited an earlier DNA damage response (measurable at 2 hours) than granulosa cells (measurable at 4 hours), as quantified by the comet assay. Granulosa cell-enriched populations were more sensitive however, responding with greater levels of DNA damage. …”
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2187“…METHODS: The p53-related genotoxicity and apoptosis of MSCs were evaluated using in vitro bioassay, viz., comet assay, cytokinesis-block micronucleus assay and apoptosis assay. …”
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2188por Grace, Marcy B., Singh, Vijay K., Rhee, Juong G., Jackson, William E., Kao, Tzu-Cheg, Whitnall, Mark H.“…We used neutralizing antibodies, flow cytometric functional assays of circulating innate immune cells, analysis of expression of genes related to cell cycle progression, DNA repair and apoptosis, and assessment of DNA strand breaks with halo-comet assays. Neutralization experiments indicated endogenous G-CSF but not IL-6 was involved in survival enhancement by 5-AED. …”
Publicado 2012
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2189por Hanot, Maïté, Boivin, Anthony, Malésys, Céline, Beuve, Michaël, Colliaux, Anthony, Foray, Nicolas, Douki, Thierry, Ardail, Dominique, Rodriguez-Lafrasse, Claire“…DNA damage measurements (γH2AX/comet assay) demonstrated that a transient GSH depletion in resistant SQ20B cells potentiated the effects of irradiation by initially increasing sparse DNA breaks and oxidative lesions after X-ray irradiation, while carbon ion irradiation enhanced the complexity of clustered oxidative damage. …”
Publicado 2012
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2190por Rondelli, Tommaso, Berardi, Margherita, Peruzzi, Benedetta, Boni, Luca, Caporale, Roberto, Dolara, Piero, Notaro, Rosario, Luzzatto, Lucio“…Unlike other techniques commonly employed in population studies, such as comet assay, this method can detect any kind of mutation, including point mutation, as long as it causes functional inactivation of PIG-A gene. …”
Publicado 2013
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2191por Xu, Shanshan, Chen, Guangdi, Chen, Chunjing, Sun, Chuan, Zhang, Danying, Murbach, Manuel, Kuster, Niels, Zeng, Qunli, Xu, Zhengping“…The biological consequences in γH2AX-elevated cell type were further explored with comet and TUNEL assays, flow cytometry, and cell growth assay. …”
Publicado 2013
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2192por Sousa, Mirta M. L., Zub, Kamila Anna, Aas, Per Arne, Hanssen-Bauer, Audun, Demirovic, Aida, Sarno, Antonio, Tian, Erming, Liabakk, Nina B., Slupphaug, Geir“…Conversely, repair of alkali-labile sites was apparently enhanced in the resistant cells, as substantiated by alkaline comet assay, autoribosylation of PARP-1, and increased sensitivity to PARP-1 inhibition by 4-AN or KU58684. …”
Publicado 2013
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2193por Buc, Emmanuel, Dubois, Damien, Sauvanet, Pierre, Raisch, Jennifer, Delmas, Julien, Darfeuille-Michaud, Arlette, Pezet, Denis, Bonnet, Richard“…In addition, 27.6% (n = 21/76) E. coli strains devoid of known cyclomodulin-encoding genes induced DNA damage in vitro, as assessed by the comet assay. In contrast to cyclomodulin-producing E. coli, these strains mainly belonged to A or D E. coli phylogroups, and exhibited a non significant difference in the distribution of CRC and diverticulosis specimens (22% versus 32.5%, p = 0.91). …”
Publicado 2013
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2194por De Marzi, Laura, Monaco, Antonina, De Lapuente, Joaquin, Ramos, David, Borras, Miquel, Di Gioacchino, Mario, Santucci, Sandro, Poma, Anna“…Moreover, the results confirmed the ceria nanoparticles’ capacity to protect cells when they are exposed to well-known oxidants such as H(2)O(2). A Comet assay was performed in the presence of both H(2)O(2) and CeO(2)-NPs. …”
Publicado 2013
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2195por Kwon, Yong-Ik, Abe, Kiyomi, Endo, Masaki, Osakabe, Keishi, Ohtsuki, Namie, Nishizawa-Yokoi, Ayako, Tagiri, Akemi, Saika, Hiroaki, Toki, Seiichi“…The following effects were observed upon aphidicolin treatment: a) comet assay showed induction of DNA double-strand breaks (DSBs) in mutant plants, b) TUNEL assay showed enhanced DNA breaks at the root meristem in mutant plants, c) a recombination reporter showed enhanced HR frequency in mutant calli, d) propidium iodide (PI) staining of root tips revealed an increased incidence of cell death in the meristem of mutant plants. …”
Publicado 2013
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2196por Forouzanfar, Fatemeh, Afkhami Goli, Amir, Asadpour, Elham, Ghorbani, Ahmad, Sadeghnia, Hamid Reza“…A significant increase in DNA damage (measured by the comet assay) was seen in nuclei of cells following SGD for 12 h (P < 0.001). …”
Publicado 2013
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2197por Poplawski, Tomasz, Chojnacki, Cezary, Czubatka, Anna, Klupinska, Grazyna, Chojnacki, Jan, Blasiak, Janusz“…We employed 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,8-dimethyl-imidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) because these substances are present in a great amount in cooked and fried meat. Using alkaline comet assay, we showed that the extent of the DNA damage induced by HCAs was significantly higher in H. pylori infected gastric mucosa cells than in non-infected counterparts. …”
Publicado 2013
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2198por Mouriño-Pérez, Rosa R., Linacre-Rojas, Lorena P., Román-Gavilanes, Ariana I., Lew, Thomas K., Callejas-Negrete, Olga A., Roberson, Robert W., Freitag, Michael“…MTB-3-GFP comets were observed moving in anterograde and retrograde direction along the hypha. …”
Publicado 2013
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2199por Rodrigues, Bianca P, Campagnaro, Bianca P, Balarini, Camille M, Pereira, Thiago M C, Meyrelles, Silvana S, Vasquez, Elisardo C“…METHODS: ROS production in MNC was evaluated by flow cytometry with the fluorescent dye dihydroethidium (DHE), a method that has been used to quantify the production of superoxide anion, and DNA damage was evaluated in both MNC and liver cells using the alkaline comet assay. Sildenafil-administered apoE(-/-) mice were compared with strain-matched mice administered with vehicle and with C57BL/6 wild-type (WT) mice. …”
Publicado 2013
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2200“…These cells consisted of untreated control cells, cells in the fifth, 15th, and 35th passage of cadmium-treated cells, and tumorigenic cells from nude mice using flow cytometry, Hoechst 33258 staining, comet assay, quantitative real-time polymerase chain reaction (PCR), Western blot analysis, random amplified polymorphic DNA (RAPD)-PCR, and sequence analysis. …”
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