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2701por Majid, Muhammad, Ijaz, Fatima, Baig, Muhammad Waleed, Nasir, Bakht, Khan, Muhammad Rashid, Haq, Ihsan-ul“…Gonadoprotective ability was assessed through comet assay and histomorphological examination of testes. …”
Publicado 2019
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2702por Davies, Benjamin M., Khan, Danyal Z., Mowforth, Oliver D., McNair, Angus G. K., Gronlund, Toto, Kolias, Angelos G., Tetreault, Lindsay, Starkey, Michelle L., Sadler, Iwan, Sarewitz, Ellen, Houlton, Delphine, Carter, Julia, Kalsi-Ryan, Sukhvinder, Aarabi, Bizhan, Kwon, Brian K., Kurpad, Shekar N., Harrop, James, Wilson, Jefferson R., Grossman, Robert, Curt, Armin, Fehlings, Michael G., Kotter, Mark R. N.“…It will be conducted via 4 phases, adhering to the guidance set out by the COMET (Core Outcomes in Effectiveness Trials) and JLA (James Lind Alliance) initiatives. …”
Publicado 2019
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2703por Burja, Blaž, Kuret, Tadeja, Janko, Tea, Topalović, Dijana, Živković, Lada, Mrak-Poljšak, Katjuša, Spremo-Potparević, Biljana, Žigon, Polona, Distler, Oliver, Čučnik, Saša, Sodin-Semrl, Snezna, Lakota, Katja, Frank-Bertoncelj, Mojca“…Phosphorylation of NF-κB was assessed by Western blot and DNA damage was measured using the COMET assay. OLE decreased significantly released protein levels of IL-6 and IL-8, as well as mRNA expression of E-selectin in SAA-stimulated HCAEC and reduced MMP2 levels in unstimulated cells. …”
Publicado 2019
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2704“…Also, a marked increase in all comet assay parameters was detected in ZnO-NPs treated groups. …”
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2705por Costa-Amaral, Isabele C., Carvalho, Leandro V. B., Santos, Marcus Vinicius C., Valente, Daniel, Pereira, Angélica C., Figueiredo, Victor O., de Souza, Juliana Mendonça, Castro, Vinicio S., Trancoso, Maria de Fátima, Fonseca, Antônio Sérgio A., Milagres, Vanessa G., Mendes, Michele P. R., Paiva, Maria José N., André, Leiliane C., Borges, Renato M., Menezes, Marco Antônio C., Alves, Sérgio R., Gonçalves, Eline S., Sisenando, Herbert Ary, Perini, Jamila A., Oliveira, Mônica S., Moura-Correa, Maria Juliana, Teixeira, Liliane R., Collins, Andrew R., Mattos, Rita de Cássia O. C., Sarcinelli, Paula N., Larentis, Ariane L.“…Sociodemographic data on the workers were evaluated; the concentration of benzene/toluene (B/T) in atmospheric air and individual trans,trans-muconic acid (ttMA) and S-phenylmercapturic acid (S-PMA) were measured; oxidative stress was analyzed by catalase (CAT), glutathione S-transferase (GST), superoxide dismutase (SOD), thiol groups (THIOL) and malondialdehyde (MDA); genotoxicity was measured by metaphases with chromosomal abnormalities (MCA) and nuclear abnormalities, comet assay using the enzyme formamidopyrimidine DNA glycosylase (C-FPG), and methylation of repetitive element LINE-1, CDKN2B and KLF6 genes. …”
Publicado 2019
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2706por Knol, Martine, Wang, Helena, Bloomfield, Frank, Piet, Tabitha, Damhuis, Stefanie, Khalil, Asma, Ganzevoort, Wessel, Gordijn, Sanne“…This study is registered in the Core Outcome Measures for Effectiveness (COMET) database. Registered on 30 June 2017. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13063-019-3588-9) contains supplementary material, which is available to authorized users.…”
Publicado 2019
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2707por Nemmar, Abderrahim, Al-Salam, Suhail, Beegam, Sumaya, Yuvaraju, Priya, Ali, Badreldin H.“…Additionally, aortic DNA damage assessed by Comet assay was significantly increased in CeO(2) NPs compared with saline, and in CP + saline versus saline, and all these effects were significantly aggravated by the combination of CP and CeO(2) NPs. …”
Publicado 2019
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2708por Bannik, Kristina, Madas, Balázs, Jarzombek, Marco, Sutter, Andreas, Siemeister, Gerhard, Mumberg, Dominik, Zitzmann-Kolbe, Sabine“…We evaluated the time and dose-dependent biological effects of α-radiation on several tumor cell lines by biological endpoints such as clonogenic survival, cell cycle distribution, comet assay, foci analysis for DNA damage, and calculated the absorbed dose by Monte-Carlo simulations. …”
Publicado 2019
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2709por Karabasz, Alicja, Szczepanowicz, Krzysztof, Cierniak, Agnieszka, Mezyk-Kopec, Renata, Dyduch, Grzegorz, Szczęch, Marta, Bereta, Joanna, Bzowska, Monika“…DNA integrity was determined by comet assay, cytokine profiling was performed using flow cytometer and detection of antibodies specific to PEG was performed by ELISA assay. …”
Publicado 2019
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2710por Sofi, Francesco, Dinu, Monica, Pagliai, Giuditta, Pierre, Fabrice, Gueraud, Francoise, Bowman, Jildau, Gerard, Philippe, Longo, Vincenzo, Giovannelli, Lisa, Caderni, Giovanna, de Filippo, Carlotta“…The measure of the primary outcome will be the change from baseline in DNA damage induced by fecal water using the comet assay in a cellular model. Secondary outcome measures will be changes in the profile of fecal microbiomes, global fecal and urinary peroxidation markers, and neoplastic biomarkers. …”
Publicado 2019
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2711por Sergei, Boichuk, Pavel, Dunaev, Aigul, Galembikova, Firyuza, Bikinieva, Ilmira, Nurgatina, Ilshat, Mustafin, Aida, Aukhadieva, Refat, Kurtasanov, Natalia, Andriutsa, Elena, Shagimardanova, Vera, Gorbunova“…A single-cell gel electrophoresis (Comet assay) data showed an increase of tail moment in Dox-treated GIST cells cultured in presence of BGJ398, a selective FGFR1-4 inhibitor, thereby revealing the attenuated DNA repair. …”
Publicado 2020
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2712por Lee, Joong Won, Kim, Yang Jee, Choi, Young Joo, Woo, Hae Dong, Kim, Gye Eun, Ha, Tae Kyung, Lee, Young Hyun, Chung, Hai Won“…Trypan-blue exclusion assays showed dose- and time- dependent decreases in ML-7 treated HL-60 cells (p < 0.05). Comet assays revealed a significant increase in DNA damage in HL-60 cells after treatment with 40 µM ML-7 for 2 h. …”
Publicado 2009
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2713por Ye, Jingrong, Hao, Mingqiang, Xing, Hui, Wang, Yuncong, Wang, Juan, Feng, Yi, Xin, Ruolei, Zeng, Ji, Zhao, Shuai, Hao, Yinxiao, Chen, Jing, Ruan, Yuhua, Li, Xue, Shao, Yiming, Lu, Hongyan“…Phylogenetic analysis was performed in order to classify sequences into their corresponding subtypes using an automated subtyping tool, the Context-Based Modeling for Expeditious Typing (COMET). Furthermore, the drug resistance mutations were determined using the World Health Organization list for surveillance of TDR mutations. …”
Publicado 2020
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2714por Zhang, Rongxin, Sun, Hao, Wang, Hong, Zhang, Wenxue, Geng, Kai, Liu, Qiang, Wang, Ping“…Apoptosis was detected using flow cytometry. A comet assay was used to assess DNA damage. The levels and degrees of cytochrome‐c, PARP, H2AX, caspase‐8, caspase‐3, and caspase‐9 activation were detected via western blot assay. …”
Publicado 2020
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2715por Tao, Ran, Wang, Chengzhang, Lu, Yin, Zhang, Changwei, Zhou, Hao, Chen, Hongxia, Li, WenJun“…Biological activities were evaluated through measurement of MTT assay, lactate dehydrogenase leakage assay (LDH), cell proliferation assays, cell apoptosis analysis, comet assay, wound healing assay, cell invasion and Western blot analysis. …”
Publicado 2020
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2716por Sharawi, Zeina W.“…Gene expression, protein expression, DNA damage, and TNFa level in tumors were determined by real-time PCR, western blot, comet assay, and Elisa, respectively. RESULTS: Treatment with AME induced anti-tumor effects against EST as indicated by 1) notable reduction in tumor size; 2) elevation in tissue necrosis and apoptosis, as confirmed histologically; 3) increased DNA fragmentation; 4) decreased expression of the apoptotic genes (p53, Bax and caspase 3), and increased expression of the anti-apoptotic marker Bcl2; 5) significantly upregulated cell cycle regulatory genes Cdc2 and connexin26, and; 6) decreased TNFa levels in tumor tissues. …”
Publicado 2020
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2717por Patil, Ajit, Elwitigala, Jayanthi P., Rajapaksa, Lilani, Gangakhedkar, Raman, Chaturbhuj, Devidas, Pendse, Razia, Rajapaksha, Dharshani Iruka, Rewari, B. B., Malliawadu, Nilmini, Jayamanna, Kanchana, Dombawela, Dammika, Kurle, Swarali“…Further, sequences were subjected to HIV-1 subtyping using REGA 3.0, COMET, jPHMM and, RIP online subtyping tools. Moreover, Bayesian phylogenetic analysis was employed to estimate the evolutionary history of HIV-1 subtype C in Sri Lanka. …”
Publicado 2020
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2718por D’Souza, Rohan, Villani, Linda, Hall, Chelsea, Seyoum, Meron, Kingdom, John, Krznaric, Michael, Donnolley, Natasha, Javid, Nasrin“…REGISTRATION DETAILS: http://www.comet-initiative.org/studies/details/1117.…”
Publicado 2020
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2719por Wu, Rongrong, Högberg, Johan, Adner, Mikael, Ramos-Ramírez, Patricia, Stenius, Ulla, Zheng, Huiyuan“…DNA damage was documented as increased levels of γH2AX, pCHK2, by Comet assay, AIM2 induction, and by increased DNA repair (non-homologous end joining) signaling. …”
Publicado 2020
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2720por Ramos, Jheneffer Sonara Aguiar, Pedroso, Thays Millena Alves, Godoy, Fernanda Ribeiro, Batista, Renata Elisa, de Almeida, Frankcione Borges, Francelin, Carolina, Ribeiro, Francis Lee, Parise, Michelle Rocha, de Melo e Silva, Daniela“…We evaluated farmworkers exposed to pesticides and individuals with no history of occupational exposure to pesticides. It was performed the comet assay to evaluate DNA damage. The immunophenotyping of TCD4(+) lymphocyte subpopulations in peripheral blood was performed by flow cytometry. …”
Publicado 2021
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