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2761“…Nonsignificant changes in the parameters of comet and micronucleus assays were observed between the treated and untreated groups of fishes that further indicated the safety profile of the enterocin ESmr18. …”
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2762por Aroosa, Sadaf, Sattar, Adeel, Javeed, Aqeel, Usman, Muhammad, Hafeez, Mian Abdul, Ahmad, Mehmood“…A single-cell gel electrophoresis (SCGE)-comet assay was used to investigate the genotoxic potential of ISO. …”
Publicado 2023
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2763por Arumugam, Ashokan, Phillips, Lawrence Rick, Moore, Ann, Kumaran, Senthil D., Sampath, Kesava Kovanur, Migliorini, Filippo, Maffulli, Nicola, Ranganadhababu, Bathri Narayanan, Hegazy, Fatma, Botto-van Bemden, Angie“…We have briefly summarized the recommendations and checklists, amongst others, for PPI in rheumatic and musculoskeletal research (e.g. the European Alliance of Associations for Rheumatology (EULAR) recommendations, the Core Outcome Measures in Effectiveness Trials (COMET) checklist and the Guidance for Reporting Involvement of Patients and the Public (GRIPP) checklist). …”
Publicado 2023
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2764“…DISCUSSION: We have prepared an alternative solution of the Likert scale selection, Delphi survey rounds, scoring group, and consensus definitions in case of an unexpected situation. TRIAL REGISTRATION: COMET (1547). Registered on March 23, 2020. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13063-023-07214-2.…”
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2765por Pei, Junxia, Su, Zhou, Zeng, Xin, Zhong, Ya, Zhang, Yamei, Yang, Yixi, Lu, Qiuxia, Li, Jian, Deng, Yu“…DNA double-strand breaks in the two cell lines were examined by the Comet assay, and cell apoptosis was analyzed by flow cytometry. …”
Publicado 2023
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2766por Ferreira, Raíssa de Oliveira, Guimarães, Abraão Tiago Batista, Luz, Thiarlen Marinho da, Rodrigues, Aline Sueli de Lima, Islam, Abu Reza Md. Towfiqul, Rahman, Md. Mostafizur, Ragavendran, Chinnasamy, Kamaraj, Chinnaperumal, Charlie-Silva, Ives, Durigon, Edison Luiz, Braz, Helyson Lucas Bezerra, Arias, Andrés Hugo, Santiago, Omar Cruz, Barceló, Damià, Malafaia, Guilherme“…On the other hand, exposure to PE MPs induced a genotoxic effect (assessed by the comet assay), as well as an increase in enzyme activity alpha-amylase, alkaline phosphatase, and carboxylesterases. …”
Publicado 2023
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2767Screening and identification of emodin as an EBV DNase inhibitor to prevent its biological functionspor Wu, Chung-Chun, Chen, Mei-Shu, Lee, Ting-Ying, Cheng, Yu-Jhen, Tsou, Hsiao-Hui, Huang, Tze-Sing, Cho, Der-Yang, Chen, Jen-Yang“…We verified the efficacy of emodin using the conventional DNA-cleavage assay. Furthermore, using comet assay and micronucleus formation detection, we confirmed that emodin can inhibit DNase-induced DNA damage and genomic instability. …”
Publicado 2023
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2768por Bravington, Alison, Obita, George, Baddeley, Elin, Johnson, Miriam J., Murtagh, Fliss E. M., Currow, David C., Boland, Elaine G., Nelson, Annmarie, Seddon, Kathy, Oliver, Alfred, Noble, Simon I. R., Boland, Jason W.“…AIMS: To develop a Core Outcome Set for the assessment of inoperable malignant bowel obstruction with clinician, patient and caregiver involvement, using COMET methodology (Core Outcome Measures in Effectiveness Trials). …”
Publicado 2023
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2769por Murata, Yasumasa, Suzuki, Kenichiro, Shigeta, Yoshiyuki, Iso, Takako, Hirose, Nozomu, Umano, Takaaki, Horibata, Katsuyoshi, Sugiyama, Kei-ichi, Hirose, Akihiko, Masumura, Kenichi, Matsumoto, Mariko“…The results of some chromosomal aberrations test and comet assay in vitro also demonstrated the genotoxic positive results. …”
Publicado 2023
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2770“…RESULTS: Interventions included the Cannabis Cessation Program (CCP), the Icelandic version of the Motivation to Change Inventory for Adolescents, the Norwegian multisystemic therapy program (MST), the Structured Interview Manual UngDOK implemented in the Swedish Maria clinics, the Finnish ADSUME-based intervention in school health care, and the Swedish Comet 12–18 and ParentStep 13–17 programs. Many interventions had originated in the US rather than in the Nordic countries and most of them were adapted from adult interventions when youth specificity was lacking. …”
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2771Urolithin C alleviates pancreatic β-cell dysfunction in type 1 diabetes by activating Nrf2 signaling“…Cell apoptosis, proliferation and DNA fragmentation were examined by Annexin V-FITC and PI staining, EdU incorporation and comet assays. Keap1, Nrf2, HO-1 and NQO1 were examined by western blot. …”
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2772por Gualtieri, Maurizio, Andrioletti, Manuela, Mantecca, Paride, Vismara, Claudio, Camatini, Marina“…A549 cells exposed to TD organic extract for 72 h presented a modified morphology, a decrease in cell proliferation and an increase in DNA damage as shown by comet assay. The dose 80 μg/ml of TD extract produced 14.6% mortality in X. laevis embryos and 15.9% mortality at 120 μg/ml. …”
Publicado 2005
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2773por Parliament, M B, Allalunis-Turner, M J, Franko, A J, Olive, P L, Mandyam, R, Santos, C, Wolokoff, B“…Independent validation of the presence of radiobiologically hypoxic cells in M006 xenografts was undertaken using the comet assay. Northern blotting analyses of monolayer cells demonstrated significant up-regulation of VEGF mRNA in the M006X line at oxygen concentrations of 6% and below. …”
Publicado 2000
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2774por De Coster, Sam, Koppen, Gudrun, Bracke, Marc, Schroijen, Carmen, Den Hond, Elly, Nelen, Vera, Van de Mieroop, Els, Bruckers, Liesbeth, Bilau, Maaike, Baeyens, Willy, Schoeters, Greet, van Larebeke, Nik“…Effect biomarkers were prostate specific antigen, carcinoembryonic antigen and p53 protein serum levels, number of micronuclei per 1000 binucleated peripheral blood cells, DNA damage (comet assay) in peripheral blood cells and 8-hydroxy-deoxyguanosine in urine. …”
Publicado 2008
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2775“…DNA damage was analysed by histone γH2AX and Comet assays. RESULTS: We found that NVP-AUY922 and NVP-BEP800 enhanced radiosensitivity in all tested cell lines. …”
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2776por Bourdon, Julie A, Saber, Anne T, Jacobsen, Nicklas R, Jensen, Keld A, Madsen, Anne M, Lamson, Jacob S, Wallin, Håkan, Møller, Peter, Loft, Steffen, Yauk, Carole L, Vogel, Ulla B“…SB in BAL cells, whole lung and liver were assessed using the alkaline comet assay. Formamidopyrimidine DNA glycosylase (FPG) sensitive sites were assessed as an indicator of oxidatively damaged DNA. …”
Publicado 2012
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2777por Khatri, Madhu, Bello, Dhimiter, Pal, Anoop K, Cohen, Joel M, Woskie, Susan, Gassert, Thomas, Lan, Jiaqi, Gu, April Z, Demokritou, Philip, Gaines, Peter“…No significant DNA damage was found at any concentration with the comet assay. Up-regulation of key DNA damage and repair genes, Ku70 and Rad51, were also observed in THP-1 cells, albeit not statistically significant. …”
Publicado 2013
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2778por Naik, Sumanta Kumar, Mohanty, Soumitra, Padhi, Avinash, Pati, Rashmirekha, Sonawane, Avinash“…Genotoxicity was studied by DAPI staining and COMET assay using mouse macrophage RAW264.7 cell line. …”
Publicado 2014
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2779por Viau, Cassiana Macagnan, Moura, Dinara Jaqueline, Facundo, Valdir Alves, Saffi, Jenifer“…The mechanisms of cell death and DNA damage induction were investigated by flow cytometry and comet assay, respectively. RESULTS: The results indicated that TTHL induced a time- and concentration-dependent growth inhibition in all human cancer cell lines. …”
Publicado 2014
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2780“…RESULTS: The obtained data showed that the PPA-treated group demonstrated higher plasma vitamin D levels compared to the control rats, together with multiple signs of brain toxicity, as indicated by a depletion of serotonin (5HT), an increase in IFN-γ and inhibition of glutathione-s-transferase activity as three biomarkers of brain dysfunction. Additionally, Comet DNA assays showed remarkably higher tail length, tail DNA % damage and tail moment as a neurotoxic effect of PPA. …”
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