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321“…There was significant increase in the comet parameters viz. mean comet tail length and frequency of cells showing migration in exposed workers as compared to controls (72.22 ± 20.76 vs. 46.92 ± 8.17, P<0.001; 31.79 vs. 5.77, P<0.001). …”
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322“…We have developed an approach combining single-cell gel electrophoresis (comet) with fluorescence in situ hybridization (FISH) that enables the comparative quantification of low, physiologically relevant levels of DNA lesions in the respective strands of defined nucleotide sequences and in the genome overall. …”
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323por Mueller, Jan, Pfanzelter, Julia, Winkler, Christoph, Narita, Akihiro, Le Clainche, Christophe, Nemethova, Maria, Carlier, Marie-France, Maeda, Yuichiro, Welch, Matthew D., Ohkawa, Taro, Schmeiser, Christian, Resch, Guenter P., Small, J. Victor“…Several pathogens induce propulsive actin comet tails in cells they invade to disseminate their infection. …”
Publicado 2014
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324“…Severe fragmentation of DNA during apoptosis can be readily measured by the neutral comet assay. Migration of DNA fragments of apoptotic cells by the electrical field can produce comet-like images. …”
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325“…To assess the risk of cancer, genotoxicity assays, including comet, micronucleus (MN), chromosomal aberration, bacterial reverse, and sister chromatid exchange assay, can be performed. …”
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326por Cortés-Gutiérrez, Elva I., López-Fernández, Carmen, Fernández, José Luis, Dávila-Rodríguez, Martha I., Johnston, Stephen D., Gosálvez, Jaime“…Key Concepts: The two-dimensional Two-Tailed Comet assay (TT-comet) protocol is a valuable technique to differentiate between single-stranded (SSBs) and double-stranded DNA breaks (DSBs) on the same sperm cell. …”
Publicado 2014
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327por Ge, Jing, Prasongtanakij, Somsak, Wood, David K., Weingeist, David M., Fessler, Jessica, Navasummrit, Panida, Ruchirawat, Mathuros, Engelward, Bevin P.“…Here, we describe a novel platform that exploits microfabrication techniques to pattern cells in a fixed microarray. The ‘CometChip’ is based upon the well-established single cell gel electrophoresis assay (a.k.a. the comet assay), which estimates the level of DNA damage by evaluating the extent of DNA migration through a matrix in an electrical field. …”
Publicado 2014
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328por Scheiman, Mitchell, Gwiazda, Jane, Zhang, Qinghua, Deng, Li, Fern, Karen, Manny, Ruth E., Weissberg, Erik, Hyman, Leslie“…METHODS: In total 469, 6 to <12-year-old, children were enrolled in COMET. Measurements of refractive error, CC (D), CR (mm), and ocular component dimensions including AL were gathered annually. …”
Publicado 2016
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329por Mitsuya, Koichi, Nakasu, Yoko, Narita, Yoshitaka, Nakasu, Satoshi, Ohno, Makoto, Miyakita, Yasuji, Abe, Masato, Ito, Ichiro, Hayashi, Nakamasa, Endo, Masahiro“…This atypical enhanced cap tends to occur in severe peritumoral edema and may produce the characteristic bulge of a metastatic mass lesion termed the “comet tail sign” (CTS). The purpose of this study was to demonstrate the features of the CTS using MR imaging and pathological findings, and to clarify its clinical relevance. …”
Publicado 2016
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330por Di Bucchianico, Sebastiano, Cappellini, Francesca, Le Bihanic, Florane, Zhang, Yuning, Dreij, Kristian, Karlsson, Hanna L.“…We conclude that mini-gel comet assay and MN scoring using flow cytometry successfully can be used to efficiently study cytotoxic and genotoxic properties of nanoparticles.…”
Publicado 2017
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331por Herath, Damayanthi, Tang, Sen-Lin, Tandon, Kshitij, Ackland, David, Halgamuge, Saman Kumara“…CoMet utilizes coverage values and the compositional features of metagenomic contigs. …”
Publicado 2017
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332“…In the “comet pond” model, a rare combination of circumstances enables the entry and landing of pristine organic material onto a planetary surface with the creation of a pond by a soft impact and melting of entrained ices. …”
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333por Zani, Claudia, Ceretti, Elisabetta, Zerbini, Ilaria, Viola, Gaia Claudia Viviana, Donato, Francesco, Gelatti, Umberto, Feretti, Donatella“…DNA damage was investigated with comet test in saliva leukocytes taken from sputum of 3- to 6-year-old children living in Brescia, Northern Italy, collected during two consecutive winter seasons (2012–2013). …”
Publicado 2020
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334por Sampogna, Gaia, Giallonardo, Vincenzo, Del Vecchio, Valeria, Luciano, Mario, Albert, Umberto, Carmassi, Claudia, Carrà, Giuseppe, Cirulli, Francesca, Dell'Osso, Bernardo, Menculini, Giulia, Belvederi Murri, Martino, Pompili, Maurizio, Sani, Gabriele, Volpe, Umberto, Bianchini, Valeria, Fiorillo, AndreaEnlace del recurso
Publicado 2021
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335“…We utilized two complementary assays based on different measurement principles: (1) comet assay and (2) FADU (fluorimetric detection of alkaline DNA unwinding) assay. …”
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336por Attico, Eustachio, Galaverni, Giulia, Bianchi, Elisa, Losi, Lorena, Manfredini, Rossella, Lambiase, Alessandro, Rama, Paolo, Pellegrini, Graziella“…Cultivated Oral Mucosa Epithelial Transplantation (COMET) is the only autologous treatment for this pathology. …”
Publicado 2022
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337por Dekate, Kamlesh, Barpande, Suresh, Tupkari, Jagdish, Thakur, Mansee, Swain, Niharika“…DNA damage was visualized as comet assay and quantified by movement of damaged strands as length of tail. …”
Publicado 2022
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338“…EXPERIMENTAL APPROACH: For this purpose, human umbilical vein endothelial cells (HUVECs) were incubated with various concentrations of osthole (40, 60, 80, and 120 µM) 24 h before cadmium chloride (CdCl(2)) treatment (40 µM), and then DNA damage was evaluated by comet assay. Furthermore, DPPH and free thiol group assays were applied to evaluate reactive oxygen species scavenger and antioxidant capacities of osthole. …”
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