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941“…The germ cell nuclear integrity was assessed by the comet assay. Testicular histopathology was conducted to evaluate the sensitive stages. …”
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942por Attaullah, Zahoor, Muhammad Kashif, Zahoor, Muhammad Asif, Mubarik, Muhammad Samee, Rizvi, Hina, Majeed, Humara Naz, Zulhussnain, Muhammad, Ranian, Kanwal, Sultana, Kishwar, Imran, Muhammad, Qamer, Samina“…However, at 25% concentration, C. murale showed highest oviposition deterrence activity (81.88%) followed by D. stramonium (79.58%). In comet assay test, at highest concentration (25%) the mean comet tail lengths represented by Penganum harmala, Datura stramonium and Azadirachta indica (Reference plant) were 10.20 ± 0.49, 9.20 ± 0.37 and 7.80 ± 0.49 μm while percent DNA damage was 10.56 ± 0.77, 10.67 ± 1.62 and 8.11 ± 0.85% respectively compared to controls cells. …”
Publicado 2020
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943por Fogaça, Manoela Viar, Cândido-Bacani, Priscila de Matos, Benicio, Lucas Milanez, Zapata, Lara Martinelli, Cardoso, Priscilla de Freitas, de Oliveira, Marcelo Tempesta, Calvo, Tamara Regina, Varanda, Eliana Aparecida, Vilegas, Wagner, de Syllos Cólus, Ilce Mara“…Materials and methods: HeLa and/or CHO-K1 cell lines were tested (3 or 24 h) in the MTT, Trypan blue exclusion, acridine orange/ethidium bromide, cytokinesis-blocked micronucleus (CBMN) and comet (36, 24 and 72 h) tests after treatment with IRN (0.1 to 200 μM) or ISA (0.5 to 50 μM). …”
Publicado 2017
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944por Smith‐Roe, Stephanie L., Wyde, Michael E., Stout, Matthew D., Winters, John W., Hobbs, Cheryl A., Shepard, Kim G., Green, Amanda S., Kissling, Grace E., Shockley, Keith R., Tice, Raymond R., Bucher, John R., Witt, Kristine L.“…Results of the comet assay showed significant increases in DNA damage in the frontal cortex of male mice (both modulations), leukocytes of female mice (CDMA only), and hippocampus of male rats (CDMA only). …”
Publicado 2019
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945por Vilchez-Aruani, Juan, Cuello-Carrión, Fernando D., Valdez, Susana R., Nadin, Silvina B.“…Cell viability was tested with resazurin assay. Comet assay results revealed no significant increase in DNA damage by NSA compared to other natural substances. …”
Publicado 2020
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946“…Phospho53BP1 foci were efficiently abolished, but γH2AX foci persisted following the suppression of APE1 activity. Comet assays demonstrated that the inhibition of APE1 decreased the DSB formation. …”
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947por Vischetti, Costantino, Casucci, Cristiano, De Bernardi, Arianna, Monaci, Elga, Tiano, Luca, Marcheggiani, Fabio, Ciani, Maurizio, Comitini, Francesca, Marini, Enrica, Taskin, Eren, Puglisi, Edoardo“…The overview was developed through the careful description and a critical analysis of three methodologies based on culture-independent approaches involving DNA extraction and sequencing (denaturing gradient gel electrophoresis, DGGE; next-generation sequencing, NGS) to characterize the microbial population and DNA damage assessment (comet assay) to determine the effect on soil invertebrates. …”
Publicado 2020
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948Direct and Oxidative DNA Damage in a Group of Painters Exposed to VOCs: Dose – Response Relationshippor Sisto, Renata, Cavallo, Delia, Ursini, Cinzia Lucia, Fresegna, Anna Maria, Ciervo, Aureliano, Maiello, Raffaele, Paci, Enrico, Pigini, Daniela, Gherardi, Monica, Gordiani, Andrea, L'Episcopo, Nunziata, Tranfo, Giovanna, Capone, Pasquale, Carbonari, Damiano, Balzani, Barbara, Chiarella, Pieranna“…Oxidative stress was determined by: urinary excretion of 8-oxodGuo, 8-oxoGua and 8-oxoGuo and direct/oxidative DNA damage in blood by Fpg-Comet assay. Multivariate linear regression models were used to assess statistical significance of the association between dose and effect biomarkers. …”
Publicado 2020
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949por Diab, Kawthar A., Ibrahim, Noha E., Fahmy, Maha A., Hassan, Emad M., Omara, Enayat A.“…As expected, CdCl(2) remarkably induced loss of histological integrity, increased DNA comet formation, increased TNF-α and p53 mRNA expression levels and increased the immunoreactivity of caspase-9 expression. …”
Publicado 2020
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950por Costerus, Sophie A., Bettink, Mark Wefers, Tibboel, Dick, de Graaff, Jurgen C., Mik, Egbert G.“…The recent introduction of the non-invasive Cellular Oxygen METabolism (COMET) monitor enables measuring in vivo mitochondrial oxygen tension (mitoPO(2)), based on oxygen-dependent quenching of delayed fluorescence of 5-aminolevulinic acid (ALA)-enhanced protoporphyrin IX. …”
Publicado 2020
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951“…The diagnostic performances of various ultrasonographic features other than comet-tail artifacts are of limited information in veterinary literatures. …”
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952por Bartoli-Leonard, Francesca, Wilkinson, Fiona L, Schiro, Andrew, Serracino Inglott, Ferdinand, Alexander, M Yvonne, Weston, Ria“…Using H(2)O(2) as a model of oxidative stress-induced DNA damage, pharmacological activation of SIRT1 reduced H(2)O(2) DNA damage-induced calcification, prevented not only DNA damage, as shown by reduced comet tail length, but also decreased yH2AX foci formation, and attenuated calcification. …”
Publicado 2020
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953por Remus, Alexandria, Smith, Valerie, Gutke, Annelie, Mena, Juan Jose Saldaña, Mørkved, Siv, Wikmar, Lena Nilsson, Öberg, Birgitta, Olsson, Christina, Robinson, Hilde Stendal, Stuge, Britt, Wuytack, Francesca“…CORE OUTCOME SET REGISTRATION: This PGP-COS was registered with COMET (Core Outcome Measures for Effectiveness Trials) in January 2017 (http://www.comet-initiative.org/studies/details/958).…”
Publicado 2021
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954“…The study has been registered on the COMET website (http://www.comet-initiative.org/Studies/Details/1677).…”
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955por Howarth, Emma, Powell, Claire, Woodman, Jenny, Walker, Erin, Chesters, Heather, Szilassy, Eszter, Gilbert, Ruth, Feder, Gene“…This study protocol adheres to reporting guidance for COS protocols and has been registered on the Core Outcome Measures for Effectiveness Trials (COMET) database. ETHICS AND DISSEMINATION: We will disseminate our findings through peer-reviewed and open access publications, the COMET website and presentations at international conferences. …”
Publicado 2021
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956por Grzesiakowska, Anna, Kasprowicz, Marek Jan, Kuchta-Gładysz, Marta, Rymuza, Katarzyna, Szeleszczuk, Olga“…Based on the percentage of the DNA content in the comet tail and the tail moment, an increase in cell DNA integrity disruption was demonstrated in all tested variants: of solution, exposure time and concentration, compared to the control sample. …”
Publicado 2021
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957por Yu, Fengting, Li, Qun, Wang, Linghang, Zhao, Hongxin, Wu, Hao, Yang, Siyuan, Tang, Yunxia, Xiao, Jiang, Zhang, Fujie“…HIV-1 subtypes and circulating recombinant forms (CRFs) were determined using the COMET online tool (http://comet.retrovirology.lu). …”
Publicado 2022
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958por Benković, Vesna, Oršolić, Nada, Knežević, Anica Horvat, Borojević, Nikola, Brozović, Gordana, Milić, Mirta“…We took kidney cortex samples after 0, 2, 6, and 24 h of exposure and measured comet parameters: tail length and tail intensity. …”
Publicado 2022
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959“…Repair percentage was obtained by Comet assay. Western blotting was employed to study the expression of repair enzymes and expression of cytokines. …”
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960por de Lima e Silva, José Rodolfo, de Lima, Anderson Rodrigues Balbino, da Silva, Demétrios Lucas, Rosa Filho, José Souto, Adam, Mônica Lúcia“…In relation to rain seasonality, the comet assay showed a greater incidence of genomic damage during the dry period, where there was a higher rate of tourist migration, compared to the rainy period. …”
Publicado 2022
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