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1261“…Using single cell alkaline gel electrophoresis (comet assay), we established the relative efficiency of cellular DNA breakage as MN > FN > QN > KL > GN. …”
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1262“…Fast prodigiosin lytic activity on individual B. subtilis cells was confirmed by a modified comet assay. The results indicate that prodigiosin autolysin induction in B. subtilis is growth phase dependent.…”
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1263“…The present results report that exposure to TiO(2)NPs produces mild to moderate changes in the cytoarchitecture of brain tissue in a time dependent manner. Moreover, Comet assay revealed the apoptotic DNA fragmentation, while PCR-SSCP pattern and direct sequencing showed point mutation of Presenilin 1 gene at exon 5, gene linked to inherited forms of the Alzheimer's disease. …”
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1264“…Since various important physical systems from particle confinement in magnetic traps to autoionization of molecular Rydberg states, through particle dynamics in accelerators and comet dynamics, can be reduced to the standard map, our results are expected to enlighten and enable an improved interpretation of diverse experimental and observational results.…”
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1265por Szyfter, Krzysztof, Stachecki, Ireneusz, Kostrzewska-Poczekaj, Magdalena, Szaumkessel, Marcin, Szyfter-Harris, Joanna, Sobczyński, Paweł“…To estimate a molecular background of adverse health effects, a cohort of 100 exposed individuals was studied by the single-cell gene electrophoresis (comet assay) test. DNA lesions in lymphocytes of the exposed group did not differ significantly compared with non-exposed blood donors. …”
Publicado 2015
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1266“…After the treatments, we assessed for DNA damage, cytotoxicity, and viability by alkaline comet, clonogenic survival, and trypan blue dye exclusion assays. …”
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1267por Gutierrez, Elva I. Cortes, Garcia-Vielma, Catalina, Aguilar-Lemarroy, Adriana, Vallejo-Ruiz, Veronica, Pina-Sanchez, Patricia, Zapata-Benavides, Pablo, Gosalvez, Jaime“…These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. …”
Publicado 2017
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1268por Prylutska, Svitlana, Politenkova, Svitlana, Afanasieva, Kateryna, Korolovych, Volodymyr, Bogutska, Kateryna, Sivolob, Andriy, Skivka, Larysa, Evstigneev, Maxim, Kostjukov, Viktor, Prylutskyy, Yuriy, Ritter, Uwe“…The genotoxicity of С(60) fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. …”
Publicado 2017
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1269por Carpentier, Rodolphe, Platel, Anne, Maiz-Gregores, Helena, Nesslany, Fabrice, Betbeder, Didier“…Genotoxicity was assessed by the micronucleus test and the comet assay and showed no increase in primary DNA damage or in chromosomal aberrations of nanoparticle vectorized pollutants. …”
Publicado 2017
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1270“…Low doses of hydroquinone (227-454 μM) reduce the viability of fibroblasts and HCT116 cells, determined by resazurin conversion, and induce genotoxic damage (DNA strand breaks), as assessed by alkaline comet assays. Bioremediation may provide an excellent alternative to promote the degradation of hydroquinone, however few microorganisms are known that efficiently degrade it. …”
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1271por Cruz, Marina Sampaio, Cabral Barroso, Sarah, Navoni, Julio Alejandro, Rocha Silva Teles, Maria Madalena, Barbosa-Filho, José Maria, de Oliveira Rocha, Hugo Alexandre, Souza do Amaral, Viviane“…Then, genotoxic and mutagenic potentials were assessed by comet assay and cytokinesis-block micronucleus assay, respectively. …”
Publicado 2016
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1272“…In addition, the γ-H2AX and comet assay demonstrated the ability of Mito-DHPs to protect against both radiation and ROS-induced DNA strand breaks. …”
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1273“…Their cytotoxicity and genotoxicity were assessed using the methyl thiazol tetrazolium (MTT) and the comet assays, respectively. The data were analyzed by Kruskal-Wallis test at the level of significance set at 0.05. …”
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1274“…Fluorescein angiography showed early hyperfluorescence areas in the PE depigmented areas associated with pigment migrations giving a comet tail appearance by gravity casting in both eyes (C,D). …”
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1275“…Sediment elutriate samples had significant effects on reproductive endpoints in G. pectinatus, and on the induction of DNA damage in cells, as shown by the comet assay. The results indicate that sediment contamination at the Ahuriri Estuary has the potential to impact biological processes of benthic and pelagic organisms. …”
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1276“…One hundred and fourteen premenopausal, apparently healthy women were included in the study: 88 obese individuals and 26 controls. The comet assay was used to measure basal DNA damage. …”
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1277Publicado 2012“…Apoptotic induction was also verified by DNA accumulation using propidium iodide nucleus staining in the S phase by flow cytometry, as well as by DNA fragmentation using the comet assay. Regarding the molecular mechanism of apoptosis induction, the gene expression of caspase-3 and caspase-9 increased after coix polysaccharide treatment. …”
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1278“…In this review, clarifications on astrochemistry, comet chemistry, laboratory astrophysics and meteoritic research with respect to organic and metalorganic chemistry will be given. …”
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1279“…The studied parameters were DNA damage evaluation using comet assay in liver and testes cells and micronucleus test in bone marrow; and histopathological examination of liver and testes tissues. …”
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1280“…The aim of this study was to investigate genotoxicity and cytotoxicity of some orthodontic glass ionomer cements commercially available by means of the single cell gel (comet) assay. For this purpose, five commercial orthodontic glass ionomer cements (Vidrion C®, Meron®, Optiband®, Multicure® and Ultra Band Lok®) were tested in murine fibroblasts in vitro. …”
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