“…The presence of Candida spp. was investigated in fecal, gastric fluid, and blow samples from each dolphin twice.  : Samples were cultured on Sabouraud Dextrose Agar and ChromAgar Candida at 30°C for 5 days. …”
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“…The lung biopsy sample was submitted to Microbiology Service of Department of Microbiology at NIH Clinical Center for Fungal culture. Antifungal susceptibility testing was conducted in accordance with the Clinical and Laboratory Standards Institute CLSI M38-A3 guidelines. …”
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“…Determination of enzyme activity: filter paper discs (diameter 5.0 mm) were impregnated with 10 μL of pure culture with a density of 0.5 McF and laid out on the surface of the nutrient medium, cultivated at + 37°C for 7 days. …”
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“…For instance, we disrupted the SAPIO_CDS2806 gene, an ortholog of sidD which drives the production of the extracellular hydroxamate-type siderophore fusarinin C in Aspergillus fumigatus. A comparison of culture supernatants from sidD mutants and their parent strain revealed that S. apiospermum secretes a unique extracellular siderophore, namely Nα‐methylcoprogen B and that sidD gene was essential for the biosynthesis of this siderophore. sidD mutation resulted in the lack of growth under iron limiting conditions. …”
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“…These projects are diverse and include medical conditions, socioeconomic activities, working conditions, information technology, food conditions, culture as well as education. According to previous studies, medical conditions are excellent, but well-being of the mental health of people in developed countries is higher in rural areas than in urban areas. …”
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“…The presence of EV-ADSCs or EV-BMMSCs in the hiPSC-CM culture along with hypertrophic stimuli reduced the protein expressions of hypertrophic specific markers (ANF, MIF, cTnI, COL1A1) and the gene expressions of IL-6 molecule involved in inflammatory process associated with cardiac hypertrophy and transcription factors SMAD2, SMAD3, cJUN, cFOS with role in cardiomyocyte hypertrophic response induced by AngII and TGF-β1. …”
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“…Cellular oxidative stress, caspase 3/7 activity, nuclear condensation, lysosomal activity, and DNA synthesis induced by F-UBA in blood cell cultures and oral epithelial squamous cell carcinoma (CLS-354) cell line were investigated through complex flow cytometry analyses. …”
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“…This seasonal phenology continues to be expressed in human-modified landscapes and, while suppressed, it also partially expresses in long-term cultures. We subsequently demonstrate that B. dorsalis, across both its endemic and invasive ranges, shows a very similar seasonality although reversed in the northern hemisphere. …”
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“…The purified cells were cultured to confluence, treated with or without tamoxifen to induce CRE expression, then treated with differentiation cocktail. …”
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“…To this end, primary hypothalamic astrocyte cultures were treated with palmitic acid (PA; 0.5 mM), oleic acid (OA; 0.5 mM) or vehicle for 24 hours and exosomes were isolated from the media and applied (1.25 or 2.50 µg/mL) to a POMC neuronal cell line for 24 hours. …”
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“…Given it was recently demonstrated that DRD2 mediates a reduction of p-AKT in a subset of NF-PitNETs and in rat prolactin-secreting tumor cells MMQ through a β-arrestin 2-dependent mechanism, the aim of the present work was to test the efficacy of adjuvant treatment with cabergoline in increasing sensitivity of human primary cultured NF-PitNET cells to antiproliferative effects of everolimus. …”
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“…We measured de novo fatty acid synthesis in cultured patient and control fibroblast cells with 13C-Glucose, with and without the C75 fatty acid synthase inhibitor. …”
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“…Thus, the aim of this study is to investigate how bisphenols affect granulosa cell viability and through which apoptotic pathway. In vitro cultured granulosa cells were exposed for 12 or 48 hrs to six treatment groups: control, vehicle, estradiol, BPA, BPS and BPF at a low dose (0.005 mg/mL) and at the BPA Lowest Observed Adverse Effect Level (LOAEL-0.05 mg/mL). …”
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“…Furthermore, AKR1C3 is implicated in castration resistant prostate cancer (CRPC) drug resistance on the basis that it is upregulated in CRPC tumors and metastases and overexpressed in cell culture & xenograft models of Abiraterone and Enzalutamide resistance, where resistance is surmounted by AKR1C3 knockdown or treatment with the AKR1C3 inhibitor indomethacin. …”
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“…We further hypothesized that androgens would downregulate insulin signaling pathway components in male vWAT adipocytes dependent on E(2) levels. vWAT and subcutaneous white adipose tissue (scWAT) were collected from male and female rats, digested in sterile-filtered collagenase, and the resulting preadipocytes were proliferated to 80% confluence. After cell cultures were induced to a minimum of 60% adipocyte cells, cells were exposed to the following treatments: no steroid, 5a-dihydrotestosterone (DHT), E(2), Test, fulvestrant, Test + letrozole, and Test + fulvestrant for 48 hours. …”
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“…Then, HSP70 knockdown in MDA-MB-231 cells were performed using siRNA. Macrophages cultured with HSP70 suppressed conditioned media from MDA-MB-231 cells showed decreased expression of CD163, a marker of pro-tumorigenic macrophages. …”
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“…However, innate differences in human and animal model reproductive tracts, and the static nature of 2D cell/tissue culture techniques, necessitate continued research and development of dynamic and more complex in vitro platforms, ex vivo approaches and in vivo therapies to study and support reproductive biology. …”
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“…The media bags were off–loaded after one day and allowed to further cool for another day before inoculation. The cultured spawn was used to inoculate the media upon cooling at room temperature. …”
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“…The primary chondrocytes were co-cultured with different phenotypes of macrophages to observe the expression of collagen II and catabolic enzymes in chondrocytes. iPSC-EVs were injected intraarticularly into the rabbit with an ACLT-induced OA model. …”
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“…Each week, CARE intervention participants received two culturally and linguistically tailored diabetes self-management videos for a total of 12 weeks. …”
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